Articles tagged "Non-invasive diagnosis"

2021 American Transplant Congress
Assessment of Donor-Derived Cell-Free DNA Performance Characteristics Across the Spectrum of TCMR and ABMR After Kidney Transplant
L. Bu¹, S. Anand², A. Pai³, J. S. Bromberg⁴, T. Alhamad⁵, V. Bowers⁶, I. Moinuddin⁷, S. Ghosh⁸, W. Tian⁸, E. Stites⁹, G. Gupta⁷
¹University of Minnesota, Minneapolis, MN, ²Intermountain Medical Center, Murray, UT, ³University of Texas McGovern Medical School, Houston, TX, ⁴University of Maryland School of Medicine, Baltimore, MD, ⁵Washington University in St. Louis, St. Louis, MO, ⁶Tampa General Hospital, Tampa, FL, ⁷Virginia Commonwealth University, Richmond, VA, ⁸CareDx, Brisbane, CA, ⁹University of Colorado, Aurora, CO
*Purpose: Different donor-derived cell-free DNA (dd-cfDNA) thresholds have been reported in association with varying TCMR, ABMR and “Borderline” diagnoses. Interpretation of dd-cfDNA levels spanning active…
2021 American Transplant Congress
Kidney Transplant Rejection Can Be Diagnosed or Even Predicted by Tracking Donor Reactive T Cell Clones in Post-transplant Samples
Y. Sambandam¹, M. Kandpal¹, J. He¹, X. Huang¹, T. S. Taylor¹, A. A. Shetty², J. M. Mathew¹, J. R. Leventhal¹
¹Surgery-Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL, ²Medicine, Nephrology Division, Northwestern University Feinberg School of Medicine, Chicago, IL
*Purpose: To test if kidney transplant (KTx) rejection can be diagnosed by monitoring for donor reactive T-cell (DRTC) clones in post-transplant (post-tx) biopsy and non-invasively…
2021 American Transplant Congress
Donor-Derived Cell-Free DNA Levels Risk-Stratify Polyoma BK Viremia and Associated Clinical Events After Kidney Transplantation – Preliminary Results from ADMIRAL Study
A. Pai¹, L. Bu², J. S. Bromberg³, G. Gupta⁴, I. Moinuddin⁴, T. Alhamad⁵, V. Bowers⁶, S. Ghosh⁷, W. Tian⁷, E. Stites⁸, S. Anand⁹
¹University of Texas McGovern Medical School, Houston, TX, ²University of Minnesota, Minneapolis, MN, ³University of Maryland School of Medicine, Baltimore, MD, ⁴Virginia Commonwealth University, Richmond, VA, ⁵Washington University in St. Louis, St. Louis, MO, ⁶Tampa General Hospital, Tampa, FL, ⁷CareDx, Brisbane, CA, ⁸University of Colorado, Aurora, CO, ⁹Intermountain Medical Center, Murray, UT
*Purpose: The threshold of 10,000 copies for blood Quant BK PCR has been used as a surrogate for BK nephropathy (BKN) in kidney transplant recipients…
2021 American Transplant Congress
High Donor-Derived Cell-Free DNA Levels Predict Development of De Novo HLA Donor-Specific Antibodies After Kidney Transplantation – Data from the ADMIRAL Study
G. Gupta¹, T. Alhamad², V. Bowers³, I. Moinuddin¹, S. Ghosh⁴, J. Zeng⁴, E. Stites⁵, A. Pai⁶, J. S. Bromberg⁷, S. Anand⁸
¹Virginia Commonwealth University, Richmond, VA, ²Washington University in St. Louis, St. Louis, MO, ³Tampa General Hospital, Tampa, FL, ⁴CareDx, Brisbane, CA, ⁵University of Colorado, Aurora, CO, ⁶University of Texas McGovern Medical School, Houston, TX, ⁷University of Maryland School of Medicine, Baltimore, MD, ⁸Intermountain Medical Center, Murray, UT
*Purpose: Donor-derived cell-free DNA (dd-cfDNA) as a marker of injury can serve to quantify subclinical inflammation and molecular injury. As the release of graft genomic…
2021 American Transplant Congress
Urinary Exosomal Cystatin C and Lipopolysaccharide Binding Protein as Biomarkers for Monitoring Antibody-mediated Rejection After Kidney Transplantation
M. Kim, S. Lim, S. Shin
Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Republic of
*Purpose: This study aims to discover and validate urinary exosomal biomarkers for antibody-mediated rejection (ABMR) after kidney transplantation.*Methods: A total of 60 urine samples from…
2020 American Transplant Congress
Integrated Multicohort Analysis of Transcriptome Profiles Identifies Blood Gene Signature to Distinguish Rejection from Infection in Patients with Solid Organ Transplants
L. C. Murphy, A. Rao, M. Donato, P. Khatri
School of Medicine, Stanford University, Stanford, CA
*Purpose: Allograft rejection and infection are now leading causes of morbidity and mortality in transplant recipients. Current diagnosis relies on nonspecific markers of graft injury,…
2020 American Transplant Congress
Assessment of the Usefulness of Urine Signature to Differentiate Acute Rejection in a Prospective Kidney Transplantation Cohort
J. Seo¹, S. Jung², J. Moon², K. Jeong³, C. Kim⁴, B. Chung⁵, J. Park⁶, Y. Kim⁷, S. Lee²
¹Department of Core Research Laboratory, Medical Science Institute, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Republic of, ²Department of Internal Medicine, Division of Nephrology, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Republic of, ³Department of Internal Medicine, Division of Nephrology, College of Medicine, Kyung Hee University, Seoul, Korea, Republic of, ⁴Department of Internal Medicine, Division of Nephrology, Kyung-pook National University School of Medicine, Daegu, Korea, Republic of, ⁵Department of Internal Medicine, Division of Nephrology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea, Republic of, ⁶Department of Internal Medicine, Sungkyunkwan University, Samsung Hospital, College of Medicine, Seoul, Korea, Republic of, ⁷Department of Internal Medicine, Division of Nephrology, Busan Paik Hospital, College of Medicine, Inje University, Busan, Korea, Republic of
*Purpose: Despite many biomarker studies in the field of transplantation, development and validation of noninvasive molecular assays to diagnose disease and monitor immune activity is…
2020 American Transplant Congress
Calibration of Donor-Derived Cell-Free DNA Criteria for Rejection with Molecular Diagnoses of Kidney Transplant Biopsies
I. Moinuddin¹, D. Kumar¹, L. Kamal¹, A. King¹, L. Kang¹, M. Levy¹, S. Bobba¹, D. Massey¹, P. Halloran², G. Gupta¹
¹Virginia Commonwealth University, Richmond, VA, ²Alberta Transplant Genomics Applied Centre, Edmonton, AB, Canada
*Purpose: Traditional microscopic histopathologic assessment (HDx) can be prone to error due to inter-observer variability as well as the non-specificity of some histologic lesions. Donor-derived…
2020 American Transplant Congress
Urine Donor Derived Cell Free DNA Aids in the Diagnosis of BK Polyomavirus Nephropathy in Kidney Transplant Recipients with BK Viruria
G. Huang¹, X. Chen¹, W. Chen¹, P. Chen¹, T. Jiang², C. Wang¹, L. Chen¹
¹The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China, ²AlloDx Biotech Co., Ltd, Suzhou, China
*Purpose: BK polyomavirus nephropathy (BKVN) are important cause of dysfunction and failure of renal transplants, which is similar to T cell mediated rejection (TCMR) in…
2020 American Transplant Congress
Non-Invasive Near Infrared Fluorescence Based Monitoring of Macrophage Mediated Inflammation
H. Karagoz¹, N. Vettikattu¹, F. Selek¹, H. Kapucu¹, E. Goktas¹, L. Liu², Y. Kulahci¹, F. Zor¹, J. M. Janjic², V. S. Gorantla¹
¹Wake Forest Institute for Regenerative Medicine, Winston Salem, NC, ²Department of Pharmaceuticals, Duquesne University, Pittsburgh, PA
*Purpose: Clinical assessments for inflammation and acute rejection (AR) require regular, invasive biopsies followed by staining protocols that provide static images of tissue. Despite such…