Articles tagged "Non-invasive diagnosis"

2021 American Transplant Congress
Urinary Exosomal Cystatin C and Lipopolysaccharide Binding Protein as Biomarkers for Monitoring Antibody-mediated Rejection After Kidney Transplantation
M. Kim, S. Lim, S. Shin
Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Republic of
*Purpose: This study aims to discover and validate urinary exosomal biomarkers for antibody-mediated rejection (ABMR) after kidney transplantation.*Methods: A total of 60 urine samples from…
2021 American Transplant Congress
Donor-derived Cell-free DNA in Upper Extremity Vascular Composite Allograft Recipients
R. Kalsi¹, M. Abuzeineh², J. Littleton¹, J. T. Shores¹, D. S. Cooney¹, C. Cooney¹, R. J. Redett¹, G. Brandacher¹, D. C. Brennan²
¹Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore, MD, ²Nephrology, Johns Hopkins University, Baltimore, MD
*Purpose: Diagnosing rejection in VCA recipients relies on clinical exam and biopsy which are both easily confounded by common causes of skin irritation. dd-cfDNA in…
2021 American Transplant Congress
Donor-Derived Cell-Free DNA Performance Characteristics are Similar for Repeat and Primary Kidney Transplant Recipients
V. R. Peddi¹, S. Akkina², S. C. Jordan³, W. Tian⁴, Y. Qazi⁵
¹California Pacific Medical Center, San Francisco, CA, ²Loyola University Medical Center, Maywood, IL, ³Cedars-Sinai Medical Center, Los Angeles, CA, ⁴CareDx, Brisbane, CA, ⁵University of Southern California, Los Angeles, CA
*Purpose: Repeat kidney transplants (RKT) represent 12.4% of kidney transplants (KT) (10.9% of all living donor and 13.6% of all deceased donor). Most RKT are…
2021 American Transplant Congress
Nonadherence Post-kidney Transplant is Associated with Increased Immune Activation Detected by Peripheral Blood Gene Expression Assay
N. Singh¹, A. Palermini¹, M. S. Naseer¹, T. Le¹, S. Tandukar¹, D. Aultman², H. Shokouh-Amiri², G. Zibari²
¹Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA, ²Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA
*Purpose: Nonadherence to immunosuppression medications is common post-transplantation. We hypothesize that nonadherence results in a heightened alloimmune response in kidney transplant recipients (KTRs) which in…
2021 American Transplant Congress
Trugraf Gene Expression Testing Can Discriminate Rejection in the Setting of Acute Kidney Injury
N. Singh¹, M. S. Naseer¹, D. Aultman², H. Shokouh-Amiri², G. Zibari²
¹Nephrology, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA, ²Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA
*Purpose: The TruGraf kidney blood gene expression test is a well-validated non-invasive biomarker to detect immune quiescence thus ruling out silent subclinical rejection in stable…
2020 American Transplant Congress
Non-Invasive Near Infrared Fluorescence Based Monitoring of Macrophage Mediated Inflammation
H. Karagoz¹, N. Vettikattu¹, F. Selek¹, H. Kapucu¹, E. Goktas¹, L. Liu², Y. Kulahci¹, F. Zor¹, J. M. Janjic², V. S. Gorantla¹
¹Wake Forest Institute for Regenerative Medicine, Winston Salem, NC, ²Department of Pharmaceuticals, Duquesne University, Pittsburgh, PA
*Purpose: Clinical assessments for inflammation and acute rejection (AR) require regular, invasive biopsies followed by staining protocols that provide static images of tissue. Despite such…
2020 American Transplant Congress
Donor-Derived Cell-Free DNA is Not Influenced by Steroid Dosing in Heart Transplant Recipients
J. Teuteberg¹, S. Krim², M. Shullo³, S. Jimenez¹, H. Eisen⁴
¹Stanford University, Stanford, CA, ²Ochsner Medical Center, New Orleans, LA, ³West Virginia University, Morgantown, WV, ⁴Pennsylvania State University, Hershey, PA
*Purpose: Gene expression profiling (GEP) was recognized as an alternative to biopsy for rejection surveillance in the 2010 International society for Heart and Lung Transplantation…
2020 American Transplant Congress
Two Pilot Studies Exploring the Diagnostic and Predictive Potential of Serum LAG3 and FGL1 in Kidney Transplant Recipients
Y. Li¹, L. Yan¹, Y. Li¹, C. Yang¹, Z. Wan¹, Y. An¹, Y. Bai¹, L. Wang¹, Y. Shi²
¹Department of Laboratory Medicine, West China Hospital, Chengdu, China, ²Department of Nephrology, West China Hospital, Chengdu, China
*Purpose: Lymphocyte activation gene 3 (LAG3), an immune checkpoint, plays an important role in the negative regulation of immune response and the induction of immune…
2020 American Transplant Congress
An Optimized Integrative Model Using Urinary Chemokines for Noninvasive Diagnosis of Acute Allograft Rejection
C. Tinel¹, A. Devresse¹, A. Vermorel¹, D. Marx², S. Caillard², C. Legendre¹, M. Rabant³, D. Anglicheau¹
¹Nephrology and Kidney Transplantation, Inserm U1151, Necker Hospital, Paris, France, ²Nephrology and Kidney Transplantation, Strasbourg University Hospital, Strasbourg, France, ³Pathology, Necker Hospital, Paris, France
*Purpose: The aim of this study was to optimize the performance of urinary chemokines C-X-C motif ligand (CXCL) 9 and CXCL10 as noninvasive diagnostic markers…
2020 American Transplant Congress
Noninvasive Detection of Subclinical Rejection in Urine RNA
K. S. Keslar¹, D. Margeta¹, J. J. Friedewald², M. Abecassis², R. B. Mannon³, P. S. Heeger⁴, R. L. Fairchild¹
¹Cleveland Clinic, Cleveland, OH, ²Northwestern University, Chicago, IL, ³University of Alabama at Birmingham, Birmingham, AL, ⁴Icahn School of Medicine, New York, NY
*Purpose: Subclinical rejection may lead to long-term graft injury and can currently only be detected by repeated biopsies. A noninvasive method is needed to monitor…