Articles tagged "Non-invasive diagnosis"

2021 American Transplant Congress
Trugraf Gene Expression Testing Can Discriminate Rejection in the Setting of Acute Kidney Injury
N. Singh¹, M. S. Naseer¹, D. Aultman², H. Shokouh-Amiri², G. Zibari²
¹Nephrology, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA, ²Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA
*Purpose: The TruGraf kidney blood gene expression test is a well-validated non-invasive biomarker to detect immune quiescence thus ruling out silent subclinical rejection in stable…
2021 American Transplant Congress
Comparison of Next Generation Sequencing Donor-derived Cell Free DNA Assay Utilizing Whole Genome Sequencing
N. Singh¹, M. S. Naseer¹, D. Aultman², H. Shokouh-Amiri², G. Zibari²
¹Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA, ²Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA
*Purpose: Circulating donor-derived cell free DNA (dd-cfDNA) is a non-invasive biomarker of kidney allograft injury with a high negative predictive value for ruling out acute…
2021 American Transplant Congress
Use of Microbial Cell Free DNA Sequencing in Solid Organ Transplant Patients
S. Chang, P. Gaynor, A. Multani, O. E. Beaird, M. Carlson, S. Yang, O. Garner, J. Schaenman
University of California, Los Angeles, Los Angeles, CA
*Purpose: Microbial cell free DNA (cfDNA) sequencing has emerged as a new noninvasive tool for the diagnosis of infectious diseases in recent years, but its…
2021 American Transplant Congress
Clinical Validation and Performance of Donor-Derived Cell-Free DNA in Allograft Rejection
S. Anand¹, A. Pai², J. S. Bromberg³, G. Gupta⁴, I. Moinuddin⁴, T. Alhamad⁵, V. Bowers⁶, S. Ghosh⁷, W. Tian⁷, L. Bu⁸, E. Stites⁹
¹Intermountain Medical Center, Murray, UT, ²University of Texas McGovern Medical School, Houston, TX, ³University of Maryland School of Medicine, Baltimore, MD, ⁴Virginia Commonwealth University, Richmond, VA, ⁵Washington University in St. Louis, St. Louis, MO, ⁶Tampa General Hospital, Tampa, FL, ⁷CareDx, Brisbane, CA, ⁸University of Minnesota, Minneapolis, MN, ⁹University of Colorado, Aurora, CO
*Purpose: Extensive literature regarding the utility of donor-derived cell-free DNA (dd-cfDNA) shares various thresholds for active rejection (AR) based on differing data sets for both…
2021 American Transplant Congress
Plasma Donor-Derived Cell-Free DNA Levels Risk-Stratify Kidney Allograft Injury with Isolated Transplant Glomerulitis
L. Bu¹, S. Anand², A. Pai³, J. S. Bromberg⁴, G. Gupta⁵, I. Moinuddin⁵, T. Alhamad⁶, V. Bowers⁷, S. Ghosh⁸, W. Tian⁸, E. Stites⁹
¹University of Minnesota, Minneapolis, MN, ²Intermountain Medical Center, Murray, UT, ³University of Texas McGovern Medical School, Houston, TX, ⁴University of Maryland School of Medicine, Baltimore, MD, ⁵Virginia Commonwealth University, Richmond, VA, ⁶Washington University in St. Louis, St. Louis, MO, ⁷Tampa General Hospital, Tampa, FL, ⁸CareDx, Brisbane, CA, ⁹University of Colorado, Aurora, CO
*Purpose: The Banff lesion Glomerulitis (g) characterizes the degree of microvascular glomerular inflammation. Some studies have demonstrated glomerulitis in the absence of C4d in association…
2020 American Transplant Congress
Discovery and Validation of a Urinary Exosome mRNA Signature for the Diagnosis of Human Kidney Transplant Rejection
R. El Fekih¹, A. Alghamdi¹, J. Hurley², C. Coticchia², J. Choi¹, H. Allos¹, S. Eskandari¹, S. Bentink², V. Tadigotla², L. Riella¹, J. Markmann³, K. Safa³, R. Formica⁴, J. Skog², J. Azzi¹
¹Medecine, Brigham and Women's Hospital, Boston, MA, ²Exosome Diagnostics Inc., Waltham, MA, ³Massachusetts General Hospital, Boston, MA, ⁴Yale School of Medecine, New Haven, CT
*Purpose: Monitoring kidney transplant rejection by repeated biopsies might result in increased procedural complications and cost. Exosomes are nanometer-sized vesicles released by cells carrying trans…
2020 American Transplant Congress
Noninvasive Imaging of CD122+ T Cells via Immuno-PET for Detection of Acute Cellular Rejection
A. J. Matar¹, B. P. Lovasik¹, D. Vanover², L. Rotolo², Y. Dong¹, D. A. Faber¹, D. V. Mathews¹, W. H. Kitchens¹, P. Santangelo², A. B. Adams¹
¹Surgery and Transplantation, Emory University, Atlanta, GA, ²Biomedical Engineering, Georgia Tech, Atlanta, GA
*Purpose: Acute cellular rejection (ACR) remains a clinically important problem following organ transplantation. Clinically, suspicion of ACR is dependent upon serum laboratory values, and diagnosis…
2020 American Transplant Congress
A Monocyte-Derived MicroRNA Signature for Antibody-Mediated Rejection in Kidney Transplantation
C. Tinel¹, B. Lamarthée¹, F. Von Tokarski¹, M. Rabant², C. Legendre¹, W. Gwinner³, M. Naesens⁴, P. Marquet⁵, D. Anglicheau¹
¹Kidney Transplantation, Inserm U1151, Necker Hospital, Paris, France, ²Pathology, Necker Hospital, Paris, France, ³Nephrology, Hannover Medical School, Hannover, Germany, ⁴Nephrology and Renal Transplantation, University Hospitals Leuven, Leuven, Belgium, ⁵Nephrology, Dialysis and Transplantation, Inserm U850, Limoges University Hospital, Limoges, France
*Purpose: MicroRNAs (miRNAs) are small noncoding RNAs which regulate mRNA expression at the posttranscriptional level. We investigated blood miRNAs as non-invasive biomarkers in kidney transplantation…
2020 American Transplant Congress
Genetic Testing of Living Related Donors for Inherited Renal Disease Improves Evaluation and Promotes Informed Choice of At-Risk Living Donors
C. P. Thomas¹, M. E. Freese¹, D. A. Katz²
¹Internal Medicine, University of Iowa, Iowa City, IA, ²Surgery, University of Iowa, Iowa City, IA
*Purpose: Living kidney donors (LKDs) with a family history of kidney disease are at higher risk of end-stage renal disease (ESRD) compared to LKDs without…
2020 American Transplant Congress
The Urine Qisant Score: Non-Invasive, Accurate, Diagnosis and Prediction of Kidney Transplant Rejection
J. Y. Yang¹, R. Sarwal¹, T. Sigdel¹, I. Damm¹, B. Rosenbaum¹, J. Liberto¹, C. Chan-On¹, J. Alberu², F. Vincenti¹, M. M. Sarwal¹
¹University of California San Francisco, San Francisco, CA, ²University of Mexico, Instituto Nacional De Ciencias Medicas y Nutricion, San Francisco, CA
*Purpose: Accurate and non-invasive monitoring of renal allografts post-transplant is essential for early detection of acute rejection (AR) and to directly impact the long-term survival…