ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Activation of Innate Immunity During Human OLT-IRI Triggers Adaptive Immunity and Correlates with the Degree of IRI Injury.

A. Zarrinpar,1 R. Sosa,2 P. Rao,2 C. Lassman,2 R. Busuttil,1 D. Gjertson,2 J. Kupiec-Weglinski,1 E. Reed.2

1Surgery, Dumont-UCLA Transplant Ctr, Los Angeles, CA
2Pathology, UCLA, Los Angeles, CA.

Meeting: 2016 American Transplant Congress

Abstract number: C124

Keywords: Immunogenicity, Inflammation, Ischemia, Lung transplantation

Session Information

Date: Monday, June 13, 2016

Session Name: Poster Session C: Ischemia Reperfusion Injury and Organ Preservation

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

Related Abstracts
  • Vascular Endothelial Cell Activation by Mitochondria Links Innate and Adaptive Immune Responses to Allograft Injury.
  • Differential Effects of the Innate vs. Adaptive Immunity in Lysing Human Renal Tubular Epithelial Cells

Ischemia reperfusion injury (IRI) in orthotopic liver transplantation (OLT) can severely compromise graft and recipient survival. We postulate this is mediated by interactions between the innate and adaptive arms of the immune system. To determine the evolution of cytokine responses following OLT, we collected blood from 53 adult OLT recipients serially: pre-operative, intraoperative blood liver flush (LF), and post-transplant on day 1, weekly for 1 month, and bi-monthly for 3 months. Graft biopsies were collected pre-transplant and 2 hours after reperfusion. Samples were analyzed using cytokine/chemokine arrays. Liver biopsies were graded for IRI (0=none to 4=severe) based on necrosis, inflammatory infiltrates, steatosis, congestion, and ballooning. A clinical IRI score based on post-transplant AST and ALT levels within the first week was used to derive an independent ranking system. 47% of transplant recipients (25/53) were IRI+ (score >2 by histopathology). IRI+ patients showed increased expression of the pro-inflammatory cytokines TNFa, IL-2, IL-8, IL-7, EGF pre-operatively. The intraoperative liver flush from IRI+ patients showed increases in innate immune cytokines IL-2, IL-8, IL-12p70 and IL-3. Early post transplant (day1) adaptive cytokines/chemokines IL-13 and IL-17a were significantly higher in IRI+ patients compared to IRI-. Late after IRI (post-week 1 and 4), adaptive cytokines/chemokines dominate the immune response in IRI+ recipients showing significant increases in IL-17a, IL-2, IL-4, IL-7, IL-3 and eotaxin. Persistent IL-1Ra was found in IRI+ recipients across most time-points tested. IRI+ patients exhibited increased IL-1a expression at week 1 post transplant that was accompanied by a significant decrease in IL-1Ra implying a resurgence of an acute inflammatory response. Three cytokines were similarly increased when analyzed by the clinical score (IL-3, IL-5, and IL-1Ra). Knowledge of innate and adaptive immunophenotypes in IRI will improve patient/donor management and refine therapeutic interventions.

CITATION INFORMATION: Zarrinpar A, Sosa R, Rao P, Lassman C, Busuttil R, Gjertson D, Kupiec-Weglinski J, Reed E. Activation of Innate Immunity During Human OLT-IRI Triggers Adaptive Immunity and Correlates with the Degree of IRI Injury. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Zarrinpar A, Sosa R, Rao P, Lassman C, Busuttil R, Gjertson D, Kupiec-Weglinski J, Reed E. Activation of Innate Immunity During Human OLT-IRI Triggers Adaptive Immunity and Correlates with the Degree of IRI Injury. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/activation-of-innate-immunity-during-human-olt-iri-triggers-adaptive-immunity-and-correlates-with-the-degree-of-iri-injury/. Accessed March 8, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Home
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Search
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Home
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.