Articles tagged "Apoptosis"

2016 American Transplant Congress
The 20S Proteasome, Active within Exosome-Like Vesicles Released by Apoptotic Cells Accelerates Rejection.
M. Dieudé,^1,4,5 C. Bell,^2,5 J. Turgeon,^1,5 D. Beillevaire,^1,4,5 S. Qi,^1,5 E. Boilard,^3,5 P. Thibault,^2,5 M.-J. Hébert.^1,4,5
¹Research Centre Centre hospitalier de l'Université de Montréal (CRCHUM), Montréal, Canada; ²Institute for research in immunology and cancer (IRIC), Montreal, Canada; ³Centre de Recherche du CHU de Québec, Québec, Canada; ⁴Université de Montréal, Montréal, Canada; ⁵Canadian National Transplant Research Program, Edmonton, Canada.
Apoptotic bodies (ApoBodies) and apoptotic exosome-like MV (ApoExo) released by apoptotic endothelial cells in vitro were purified by sequential centrifugation and analysed by electron microscopy…
2016 American Transplant Congress
Elevated Intragraft Expression of Innate Immunity and Cell Death-Related Markers Characterizes Deceased Donor Conditions and Is a Risk Factor for Adverse Graft Outcome.
M. Eikmans,¹ J. Yang,¹ G. Haasnoot,¹ C. van Kooten,² M. Mallat,² H. de Fijter,² I. Bajema,³ F. Claas.¹
¹Immunohematology, LUMC, Leiden, Netherlands; ²Nephrology, LUMC, Leiden, Netherlands; ³Pathology, LUMC, Leiden, Netherlands.
Background: The innate immune response is increasingly being recognized in allograft injury after kidney transplantation. We aimed to establish the relationship of innate immune gene…
2016 American Transplant Congress
Mechanism of FK506 Induced TNF Related Apoptosis in Jurkat T Cells.
S. Chung, S. Choi.
Department of Surgery, Chonnam National University Hospital, Gwangju, Korea.
Purpose: To elucidate the mechanism of FK506 induced TRAIL(TNF related apoptosis inducing ligand) –R1(DR4) and TRAIL-R2(DR5) in FK506-treated Jurkat T cells. And signal transduction pathway…
2016 American Transplant Congress
Cytokine Storm in Acute Post-Hepatectmy Phase Associates with Liver Injury and Impaired Liver Regeneration.
Y. Xu,^1,2 N. Navarro-Alvarez,¹ J. Dong,² J. Markmann,¹ H. Yeh.¹
¹Transplantation Division, Massachusetts General Hospital, Boston, MA; ²Department of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
Background Posthepatectomy liver failure and its transplant counterpart, small-for-size syndrome, remain poorly understood. Large increases in IL-6 are associated with poor allograft function and IL-6…
2016 American Transplant Congress
Exosome-Like Vesicles Released in Association with Tissue Injury Trigger Anti-LG3 Production.
M. Dieudé,^1,2,3 J. Turgeon,^1,3 B. Yang,^1,2,3 L. Pomerleau,^1,3 K. Hamelin,^1,3 S. Lan,^1,2,3 S. Qi,^1,3 D. Gingras,^1,3 W. Dhari,¹ A. Rivard,¹ M.-J. Hébert.^1,2,3
¹Research Centre, Centre hospitalier de l'Université de Montréal, Montréal, Canada; ²Université de Montréal, Montréal, Canada; ³Canadian National Transplantation Research Program, Edmonton, Canada.
Pre-transplant levels of autoantibodies to perlecan/LG3 predict poor allograft outcomes in renal transplant patients. We showed previously thatapoptotic exosome-like vesicles (ApoExo) enhance anti-LG3 production and…
2016 American Transplant Congress
Lysosome Ativity Regulates LG3 Export by Apoptotic Cells.
D. Beillevaire,^1,2,4 J. Turgeon,^1,2,4 E. Boilard,^3,4 M. Dieudé,^1,2,4 M.-J. Hébert.^1,2,4
¹CRCHUM, Montréal, Canada; ²Université de Montréal, Montréal, Canada; ³CHUL, Québec, Canada; ⁴CNTRP - Canadian National Transplant Research Program, Edmonton, Canada.
LG3, a C-terminal fragment of perlecan bioactive on vascular cells, has been implicated in aggravation of renal allograft rejection and vascular injury. Recently, we have…
2016 American Transplant Congress
Distinctive Roles of Gsk3 Inhibitory Phosphorylation in Liver Inflammatory Immune Response and Hepatocellular Injury Against Ischemia Reperfusion.
H. Zhou,^1,2 J. Zhu,¹ S. Yue,¹ R. Busuttil,¹ J. Kupiec-weglinski,¹ L. Lu,² Y. Zhai.¹
¹Surgery, David Geffen School of Medicine-UCLA, Los Angeles, CA; ²Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Background & AimsGlycogen synthase kinase 3 (Gsk3) a and b are ubiquitously expressed, constitutively active serine/threonine kinases, which play complex roles in cell activation, proliferation…
2015 American Transplant Congress
Human CD8⁺TCR^– Facilitating Cells Enhance HSC Engraftment In Vivo and Promote HSC Survival In Vitro
Y. Huang,¹ M. Elliott,¹ E. Yolcu,¹ T. Miller,¹ L. Bozulic,² Y. Wen,¹ H. Xu,¹ S. Ildstad.^1,2
¹Institute for Cellular Therapeutics, University of Louisville, Louisville, KY; ²Regenerex, LLC, Louisville, KY.
CD8+/TCR- facilitating cells (FC) in mouse BM significantly enhance engraftment of hematopoietic stem cells (HSC) in syngeneic and allogeneic recipients. Human FC phenotype and mechanism…
2015 American Transplant Congress
MiR-26a Regulates Mouse Hepatocyte Proliferation By Directly Targeting Their 3′ Untranslated Region of CCNE2/CCND2
J. Zhou, X. Yuan, W. Ju, X. Jiao, X. Wang, M. Han, D. Wang, X. Zhu, X. He.
Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
Objectives: The deficiency of liver regeneration needs to be addressed in the fields of liver surgery, split liver transplantation and living donor liver transplantation. Researches…
2015 American Transplant Congress
Down-Regulation of Autophagy Correlates With Increased Apoptosis in the Kidney, But Not in the Liver, of Brain Dead Rats
A. van Erp,¹ R. Rebolledo,³ P. Ottens,¹ J. Pirenne,² H. Leuvenink,¹ J.-P. Decuypere.²
¹Department of Surgery, University Medical Center Groningen, Groningen, Netherlands; ²Department of Microbiology and Immunology, Catholic University Leuven, Leuven, Belgium; ³Department of Surgery, University of Chile, Santiago, Chile.
Organs from brain dead donors have inferior quality and show higher rejection rates after transplantation compared to living donors. Brain death (BD) in the donor…