Date: Tuesday, June 14, 2016
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Halls C&D
Background: The long-term outcome of lung transplantation is impeded by obliterative bronchiolitis (OB). However, the current treatment cannot effectively prevent the process of OB. The present studies show the apoptosis of tracheal epithelia triggers the development of OB and the extracellular matrix is mainly produced by myofibroblasts. Here, we sought to investigate the relationship between the apoptosis of tracheal epithelia and the increase of myofibroblasts in the process of OB.
Methods: Mice orthotopic tracheas transplant model was established by using Balb/c and C57BL/6. The allografts and syngrafts were separately harvested at 1, 2, 3 and 4 weeks after transplantation. The percentage of trachea luminal occlusion was assayed through morphometry. Immunofluoresence staining was used to detect the apoptotic epithelium and recipient-derived myofibroblast in trachea grafts. The ecpression of SDF-1α and TGF-β in grafts was detected.
Results: We found that there were more apoptotic epithelia in allografts group, and compared with control group, the level of luminal occlusion was higher at different time points. Moreover, the increase of apoptosis of tracheal epithelia was earlier (the peak of apoptosis was at 3 weeks after transplantation) than that of tracheal luminal occlusion (the peak of occlusion was at 4 weeks after transplantation). Immunofluorescence analysis showed that myofibroblasts in allografts were derived from recipient's mesenchymal stem cells (MSCs) from 2 weeks to 4 weeks after transplantation. The expressions of SDF-1α and TGF-β were more in the epithelia of allografts than those of syngrafts.
Conclusions: Our study indicated the apoptotic tracheal epithelia in OB may increase the amount of myofibroblasts derived from recipient's MSCs. The migration of MSCs to grafts is dependent on increased expression of chemokine of SDF-1, and differentiation of MSCs into myofibroblasts is in response to higher expression of TGF-β. The therapeutic methods to prevent apoptosis of tracheal epithelia may improve the outcome of lung transplantation.
(This work was supported by the National Natural Science Foundation of China: 81300066 to Jun Nie).
CITATION INFORMATION: Wang C, Xia T, Zhang X, Jiang K, Wang J, Nie J. The Apoptosis of the Tracheal Epithelia Increase the Recipient's MSCs Derived Myofibroblasts in Allografts to Exacerbate Obliterative Bronchiolitis After Tracheal Transplantation in Mice. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:Wang C, Xia T, Zhang X, Jiang K, Wang J, Nie J. The Apoptosis of the Tracheal Epithelia Increase the Recipient's MSCs Derived Myofibroblasts in Allografts to Exacerbate Obliterative Bronchiolitis After Tracheal Transplantation in Mice. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/the-apoptosis-of-the-tracheal-epithelia-increase-the-recipients-mscs-derived-myofibroblasts-in-allografts-to-exacerbate-obliterative-bronchiolitis-after-tracheal-transplantation-in-mice/. Accessed June 5, 2020.
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