ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Elevated Intragraft Expression of Innate Immunity and Cell Death-Related Markers Characterizes Deceased Donor Conditions and Is a Risk Factor for Adverse Graft Outcome.

M. Eikmans,1 J. Yang,1 G. Haasnoot,1 C. van Kooten,2 M. Mallat,2 H. de Fijter,2 I. Bajema,3 F. Claas.1

1Immunohematology, LUMC, Leiden, Netherlands
2Nephrology, LUMC, Leiden, Netherlands
3Pathology, LUMC, Leiden, Netherlands.

Meeting: 2016 American Transplant Congress

Abstract number: 211

Keywords: Apoptosis, Biopsy, Kidney transplantation, Outcome

Session Information

Date: Monday, June 13, 2016

Session Name: Concurrent Session: Kidney Immune Monitoring 2

Session Time: 2:30pm-4:00pm

 Presentation Time: 3:30pm-3:42pm

Location: Ballroom C

Related Abstracts
  • Histopathological Criteria for Elevated Risk of Delayed Graft Function in German Pretransplantation Kidney Biopsies from Deceased Donors
  • Body Mass Index (BMI) Mismatch in Deceased Kidney Donation Is an Independent Risk Factor for Graft Failure

Background: The innate immune response is increasingly being recognized in allograft injury after kidney transplantation. We aimed to establish the relationship of innate immune gene expression at the moment of implantation or acute rejection (AR) with graft outcome.

Method: 19 genes, including TLRs, complement components and regulators, and apoptosis-related genes, were analyzed at the mRNA level in biopsies of 123 patients with AR and in paired pre-implantation biopsies (n=75). Expression levels before transplantation were tested in relation to the type of donor (deceased or living; making up 78% and 22%, respectively, of the group) and occurrence of delayed graft function (DGF). The expression levels during AR were investigated for association with steroid-responsiveness and graft loss.

Results: Before transplantation, expression of C2, C3, and the Bax/Bcl2 ratio were significantly higher (P<0.01) in deceased donors as compared to living donors. Within deceased donors there were no associations between gene expression and DGF. During AR, all TLRs and C2 and C3 showed increased expression compared with pre-implantation conditions whereas three complement regulators and C4 and Bcl2 showed decreased expression. Expression of none of the genes was associated with steroid response. However, relatively high TLR4 expression and Bax/Bcl2 ratio at time of AR were related to adverse graft outcome. Patients with a high Bax/Bcl2 ratio in their deceased donor graft (higher than that in the living donor group) had significantly (P<0.05) worse death-censored graft survival (61.3%) at 6-year post-transplant compared to those with a low ratio (89.4%) and those with a living donor graft (95.8%). In Cox regression analysis, TLR4 and Bax/Bcl2 ratio in the deceased donor group predicted outcome independently of previously identified clinical risk factors of graft loss.

Conclusion: The elevated expression of C2, C3, and Bax/Bcl2 ratio in deceased donor transplants supports the notion that complement and apoptosis pathway activity is already enhanced before kidney transplantation. The relationship of high Bax/Bcl2 ratio during AR with graft loss may point to an adverse effect of intragraft cell death, and thereby possibly enhanced immunogenic danger signals, on graft outcome.

CITATION INFORMATION: Eikmans M, Yang J, Haasnoot G, van Kooten C, Mallat M, de Fijter H, Bajema I, Claas F. Elevated Intragraft Expression of Innate Immunity and Cell Death-Related Markers Characterizes Deceased Donor Conditions and Is a Risk Factor for Adverse Graft Outcome. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Eikmans M, Yang J, Haasnoot G, Kooten Cvan, Mallat M, Fijter Hde, Bajema I, Claas F. Elevated Intragraft Expression of Innate Immunity and Cell Death-Related Markers Characterizes Deceased Donor Conditions and Is a Risk Factor for Adverse Graft Outcome. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/elevated-intragraft-expression-of-innate-immunity-and-cell-death-related-markers-characterizes-deceased-donor-conditions-and-is-a-risk-factor-for-adverse-graft-outcome/. Accessed March 1, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Left-Sided Donor Nephrectomy Predisposes Living Kidney Donors to Latent Adrenal Insufficiency with Symptoms of Fatigue and Inferior Quality of Life.
  • Home
  • Search
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.