ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Tolerant Pediatric Liver Transplant (LT) Recipients (TOL) from WISP-R (NCT00320606) Split into Two Phenotypically Distinct Groups by PD1 Expression on CD4+ T Cells at Baseline, Prior to Immunosuppression Withdrawal (ISW).

B. Burrell,1 S. Feng,2 N. Bridges,3 U. Ekong,4 S. Kanaparthi,1 A. Kirk,5 A. Long,1 K. Spain,6 L. Stempora,5 L. Turka,1 N. Tchao.1

1ITN, Bethesda
2UCSF, San Francisco
3NIAID, Bethesda
4Yale, New Haven
5Duke, Durham
6Rho, Chapel Hill

Meeting: 2017 American Transplant Congress

Abstract number: 194

Keywords: Hyporeactivity, Liver transplantation, T cells, Tolerance

Session Information

Date: Sunday, April 30, 2017

Session Name: Concurrent Session: Tolerance: Clinical Studies

Session Time: 4:30pm-6:00pm

 Presentation Time: 4:30pm-4:42pm

Location: E353C

Related Abstracts
  • Steroid Withdrawal and De Novo Donor-Specific HLA Antibodies in Renal Transplant Patients: A Prospective, Randomized, Controlled Study in Parallel Groups. Preliminary Results.
  • Primary Outcome of iWITH: A Multi-Center Clinical Trial of Complete Immunosuppression Withdrawal (ISW) in Stable Pediatric Liver Transplant (LT) Recipients.

Background: Combinations of cell surface markers such as PD1, CD57, and/or KLRG1 identify T cells that may fail to respond to cognate antigen challenge – a potential mechanism of organ transplant tolerance. We hypothesized that, prior ISW, TOL and non-TOL would differ in these cell surface markers. We tested 12 TOL and 7 non-TOL subjects identified by WISP-R, a multi-center ISW trial for children ≥4yrs after parental living donor LT.

Methods: Frozen, banked, pre-ISW PBMC samples were analyzed by flow cytometry (FlowJo software). Clinical characteristics of non-, HI- and LO-TOL were compared using Kruskal Wallis or Fisher's exact tests.

Results: Prior to ISW, TOL compared to non-TOL had increased CD4+PD1+cells (28 vs 16%; p=0.042; Figure). Notably, TOL separated into 2 groups based on high (HI-TOL; n=6) vs low (LO-TOL; n=6) percentage of CD4+PD1+cells. HI-TOL (38% CD4+PD1+) were distinct from LO-TOL (17%; p=0.001) and non-TOL (16%; p=0.0004); in contrast, LO-TOL were similar to non-TOL (p=0.81) (Figure; Table 1). Expanded flow panels showed similar patterns for CD4+PD1+CD57-KLRG1- and memory cells. Differences between HI- and LO-TOL did not correlate with clinical surrogates of antigen exposure: age at LT or screening, time since LT [HI-TOL tended to be younger at screening (p=0.078) and earlier after LT (p=0.11)], rejection history, EBV/CMV status, or presence of α-Class II donor specific antibody (Table 2).

Conclusions: Prior to ISW, CD4+T cell profiles separated TOL from non-TOL and divided TOL into HI- and LO-TOL based on PD1 expression. HI- compared to LO-TOL appear more antigen-experienced, suggesting hyporesponsiveness/exhaustion may contribute to tolerance in a subgroup of pediatric LT recipients. Future analyses will use additional costimulatory/coinhibitory markers to further define this potentially hyporesponsive PD1+ cell population.

CITATION INFORMATION: Burrell B, Feng S, Bridges N, Ekong U, Kanaparthi S, Kirk A, Long A, Spain K, Stempora L, Turka L, Tchao N. Tolerant Pediatric Liver Transplant (LT) Recipients (TOL) from WISP-R (NCT00320606) Split into Two Phenotypically Distinct Groups by PD1 Expression on CD4+ T Cells at Baseline, Prior to Immunosuppression Withdrawal (ISW). Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Burrell B, Feng S, Bridges N, Ekong U, Kanaparthi S, Kirk A, Long A, Spain K, Stempora L, Turka L, Tchao N. Tolerant Pediatric Liver Transplant (LT) Recipients (TOL) from WISP-R (NCT00320606) Split into Two Phenotypically Distinct Groups by PD1 Expression on CD4+ T Cells at Baseline, Prior to Immunosuppression Withdrawal (ISW). [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/tolerant-pediatric-liver-transplant-lt-recipients-tol-from-wisp-r-nct00320606-split-into-two-phenotypically-distinct-groups-by-pd1-expression-on-cd4-t-cells-at-baseline-prior-to-immunosuppress/. Accessed January 17, 2021.

« Back to 2017 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.