Protective Effect of Crepidiastrum denticulatum Extract Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice.
Surgery, University of Ulsan, Gangneung Asan Hospital, Gangneung, Gangwon, Korea
Anesthesia, University of Ulsan, Gangneung Asan Hospital, Gangneung, Gangwon, Korea
Surgery, University of Ulsan, Gangneung Asan Hospital, Gangneung, Gangwon, Korea
Pathology, University of Ulsan, Gangneung Asan Hospital, Gangneung, Gangwon, Korea
Surgery, University of Ulsan, Gangneung Asan Hospital, Gangneung, Gangwon, Korea
Surgery, University of Ulsan, Gangneung Asan Hospital, Gangneung, Gangwon, Korea
Natural Medicine Center, Korea Institute of Science and Technology, Gangneung, Gangwon, Korea
Natural Medicine Center, Korea Institute of Science and Technology, Gangneung, Gangwon, Korea
Meeting: 2017 American Transplant Congress
Abstract number: A169
Keywords: Apoptosis, Inflammation, Ischemia, Liver
Session Information
Session Name: Poster Session A: Ischemic Injury and Organ Preservation Session I
Session Type: Poster Session
Date: Saturday, April 29, 2017
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall D1
Crepidiastrum denticulatum (CD) is a well-known, traditionally consumed vegetable in Korea, which was recently reported to have bioactive compounds with detoxification and antioxidant properties. Ischemia-reperfusion injury (IRI) is a major critical event that commonly occurs after liver transplantation. We investigated whether CD protects against IR-induced acute liver injury in mice.
Partial (70%) hepatic IRI was induced in male C57BL/6 mice by portal triad pedicle occlusion for 45 min followed by reperfusion for 6 h. CD (75 mg/kg BW, oral) was administered 5 days before the IRI.
Treatment with CD significantly decreased serum sALT, sAST and LDH as well as histological changes . CD prevented hepatic GSH depletion and increased MDA levels. Western blotting indicated that CD significantly increased the levels of Atg7, LC3-I/II, attenuated PARP and caspase3. The expression of the Toll-like receptor 4, iNOS and NFkB were decreased in the CD treatment group .
CD improved the acute hepatic IRI by reducing oxidative damage, apoptosis, inflammation and inducing autophagy. These findings suggest that CD is a promising agent against acute IR-induced hepatic damage.
CITATION INFORMATION: Jang H, Lee H, Park C, Eom D, Lee J, Oh M, Yoo J, Rho C. Protective Effect of Crepidiastrum denticulatum Extract Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Jang H, Lee H, Park C, Eom D, Lee J, Oh M, Yoo J, Rho C. Protective Effect of Crepidiastrum denticulatum Extract Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/protective-effect-of-crepidiastrum-denticulatum-extract-pretreatment-against-hepatic-ischemia-reperfusion-injury-in-mice/. Accessed December 2, 2024.« Back to 2017 American Transplant Congress