Date: Saturday, May 30, 2020
Session Time: 3:15pm-4:00pm
Presentation Time: 3:30pm-4:00pm
*Purpose: Organ ischemia reperfusion injury (IRI) is unavoidable in kidney transplantation and induces reactive oxygen species and causes organ damage. Although the effectiveness of hydrogen for IRI has been reported for a long time, oral ingestion of hydrogen water and inhalation of hydrogen gas are difficult to apply widely in terms of efficiency and general use respectively. We succeeded in generating a large amount of hydrogen molecules by crushing silicon to nano-sized particles and reacting with water. A silicone component agent was administered to a rat model of renal IRI to examine whether renal injury could be suppressed.
*Methods: Six-week-old male SD rats were administered a silicon component feed containing 1% nano-sized silicon particles and IRI surgery was performed one week later. This group was made into a silicon component group (IRI + Si group). An IRI group that administers normal diet and performs IRI surgery and a sham group that performs only right nephrectomy were prepared, and each group was compared 72 hours after surgery.
*Results: Serum creatinine and urine protein significantly decreased in the IRI + Si group,compared to the IRI group. Serum malondialdehyde and urinary 8-hydroxydeoxyguanosine, which are oxidative stress markers, were also significantly decreased in the IRI + Si group. Transcriptome analysis by microarray showed that in the IRI + Si group, the expression of genes related to immune response, cytokine production, and apoptotic signal was decreased in comparison with the IRI group. Furthermore, suppression of mRNA expression level of inflammatory cytokines by quantitative PCR and reduction of renal tubular epithelial cell apoptosis by immunohistochemical evaluation were obtained, suggesting anti-inflammatory and anti-apoptotic effects.
*Conclusions: Rat renal IRI was attenuated by oral administration of nano-sized silicon particles, which is a novel hydrogen administration method.
To cite this abstract in AMA style:Kawamura M, Imamura R, Kobayashi Y, Taniguchi A, Nakazawa S, Kato T, Abe T, Kobayashi H, Nonomura N. Oral Administration of Nano-Sized Silicon Particles Regulates Gene Expression Related to Oxidative Stress and Attenuates Rat Renal Ischemia Reperfusion Injury [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/oral-administration-of-nano-sized-silicon-particles-regulates-gene-expression-related-to-oxidative-stress-and-attenuates-rat-renal-ischemia-reperfusion-injury/. Accessed August 7, 2020.
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