ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Matrix Metalloproteinase-9 in Human Orthotopic Liver Transplant Biopsies.

S. Duarte, N. Datta, J. Baber, R. Busuttil, A. Zarrinpar, A. Coito.

The Dumont-UCLA Transplant Center, Los Angeles.

Meeting: 2016 American Transplant Congress

Abstract number: 501

Keywords: Inflammation, Ischemia, Liver transplantation, Metalloproteinases

Session Information

Date: Tuesday, June 14, 2016

Session Name: Concurrent Session: Ischemia Reperfusion Injury: Clinical Innovation

Session Time: 4:30pm-6:00pm

 Presentation Time: 5:30pm-5:42pm

Location: Room 311

Related Abstracts
  • Matrix Metalloproteinase-2 (MMP-2) Activity Protects Against Hepatic Ischemia Reperfusion Injury Through MMP-9-Dependent and MMP-9-Independent Mechanisms.
  • Encapsulated Human Mesenchymal Stem Cells Reduce Alpha-Smooth Muscle Actin and Increase Matrix Metalloproteinase-9 Expression during Liver Fibrosis

Liver ischemia reperfusion injury (IRI) is an inflammatory event that contributes to graft dysfunction in orthotopic liver transplantation (OLT). Matrix Metalloproteinases (MMPs) are responsible for extracellular matrix (ECM) turnover in physiological and inflammatory conditions. We established that matrix metalloproteinase-9 (MMP-9) proteolytic activity facilitates inflammatory leukocyte invasion in murine models of warm and cold liver IRI. Here we characterize MMP-9 expression in biopsies of human OLT recipients. Methods: Liver biopsies were collected from 26 OLT recipients after reperfusion and prior to abdominal closure. In 5 cases, ischemic donor livers were also biopsied prior to transplant. Controls were biopsied from non-lesion liver of patients subject to liver resection. Biopsies were fixed in formalin prior to MMP-9 detection. Results: 15 male and 11 female recipients had a median age of 63, a mean MELD score of 28, with mean CIT and WIT of ~7h and ~1h, respectively. Triple immunofluorescence confirmed CD45+ infiltrating inflammatory leukocytes as the major sources of MMP-9 in human OLTs. MMP-9+ leukocytes were modestly detected in biopsies of both non-lesion controls and cold ischemic livers, and were significantly increased (~2-fold) in liver biopsies after OLT (MMP-9; OLT vs. CI vs. non-lesion: 32±5 vs. 17±3 vs. 11±4; p<0.002). Leukocyte MMP-9 expression had a positive correlation with liver injury post-OLT; MMP-9+ leukocyte numbers correlated with serum AST and ALT levels at post-operative day 5 (POD5) (AST: R=0.457, p<0.04; ALT: R=0.524, p<0.02) and 7 (POD7) (AST: R=0.407, ns; ALT: R=0.570, p<0.01). Moreover, recipients with serum transaminases elevated above the upper limit of normal (AST>48 & ALT>55 IU/L) had increased MMP-9+ leukocytes compared to recipients within normal AST and ALT range at POD5 (MMP-9: 37±16 vs. 8±6; p<0.02) and POD7 (MMP-9: 31±9 vs. 15±13; p<0.05). MMP-9+ leukocytes were also increased in reperfusion biopsies from steatotic donor grafts (~30% mean macrosteatosis) compared to reperfusion biopsies from non-steatotic donor grafts (MMP-9: 44±26 vs. 19±12; p<0.03). Conclusion: Our results show that MMP-9 is produced by infiltrating inflammatory leukocytes in human OLT biopsies and that its expression associates with liver injury and graft steatosis. Overall, this study correlates with our previous findings in murine models of liver IRI and together support an important role for MMP-9 expression in liver transplantation.

CITATION INFORMATION: Duarte S, Datta N, Baber J, Busuttil R, Zarrinpar A, Coito A. Matrix Metalloproteinase-9 in Human Orthotopic Liver Transplant Biopsies. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Duarte S, Datta N, Baber J, Busuttil R, Zarrinpar A, Coito A. Matrix Metalloproteinase-9 in Human Orthotopic Liver Transplant Biopsies. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/matrix-metalloproteinase-9-in-human-orthotopic-liver-transplant-biopsies/. Accessed February 25, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Left-Sided Donor Nephrectomy Predisposes Living Kidney Donors to Latent Adrenal Insufficiency with Symptoms of Fatigue and Inferior Quality of Life.
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.