Date: Saturday, June 1, 2019
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall C & D
*Purpose: Despite significant advances in organ transplantation, damage caused to organs due to long cold ischemia time and perfusion solutions remains a serious hurdle. The objective of this preliminary experimental animal study was to compare the efficacy of the new Baskent University Preservation Solution (BUPS) with UW, HTK and saline solutions.
*Methods: In addition to the electrolytes (Na, K, Cl, Mg), the following was used in BUPS: raffinose, the trisaccharide composed of galactose, glucose and fructose was used as an energy source; mannitol as an osmoregulator; N-acetylcycteine as an antioxidant, an antiapoptotic, and a microsomal glutathione transferase substrate increasing the cellular pools of free radical scavengers; taurine, a sulfonated amino acid, as a membrane stabilizer, an antioxidant protecting against ischemia-reperfusion injury, an intracellular calcium regulator, and an osmoregulator; adenosine, a purine riboside composed of adenine molecule attached to a ribose sugar molecule, as an energy source, a blood flow regulator, an antiplatelet, an anti-inflammatory agent, and a neuromodulator; ascorbic acid, a cofactor for multiple enzymes, serving as an electron donor for mono- and di-oxygenases, and as a strong antioxidant. 50 Male Sprague Downey rats, weighting 350-450 g, were randomized into 4 groups (Group B: BUPS, Group H: HTK, Group W: UW, and Group C: Saline) corresponding to the 3 solutions tested. Under general anesthesia, the rats were perfused with 50 cc (+4 C) BUPS, UW, and HTK perfusion solutions from the distal part by connecting the proximal of the intra-abdominal aorta after laparotomy. To assess cold ischemia injury, both kidneys, liver and intestine were removed and placed in the same solution. Samples were taken from these organs for pathological evaluation at 0, 1, 3, 6, 12, 24 and 48 hours.
*Results: Neither group had shown significant cellular injury at 0, 1, 3-hour perfusion. At 6,12, 24 and 48-hour perfusion, the percentage of injured were found to be lowest in Group B and H compared to Group W and C (p<0.01). Also, compared to Group H, the degree of organ damage was most moderate in Group B at 6,12, 24 and 48-hour perfusion (p<0.05).
*Conclusions: Cellular damage during ischemic conditions was assessed with various preservation solutions. The rate of cellular injury was lowest in BUPS therefore making it a utilizable perfusion solution.
To cite this abstract in AMA style:Haberal M, Kirnap M, Erdem R, Ozdemir H, Lux M, Bacanli D. Evaluation of New Baskent University Preservation Solution for Liver, Kidney and Intestine Graft during Cold Ischemia: Preliminary Experimental Animal Study [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/evaluation-of-new-baskent-university-preservation-solution-for-liver-kidney-and-intestine-graft-during-cold-ischemia-preliminary-experimental-animal-study/. Accessed September 27, 2020.
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