Crepidiastrum denticulatum (CD) is a well-known, traditionally consumed vegetable in Korea, which was recently reported to have bioactive compounds with detoxification and antioxidant properties. Ischemia-reperfusion injury (IRI) is a major critical event that commonly occurs after liver transplantation. We investigated whether CD protects against IR-induced acute liver injury in mice.

Partial (70%) hepatic IRI was induced in male C57BL/6 mice by portal triad pedicle occlusion for 45 min followed by reperfusion for 6 h. CD (75 mg/kg BW, oral) was administered 5 days before the IRI.

Treatment with CD significantly decreased serum sALT, sAST and LDH as well as histological changes . CD prevented hepatic GSH depletion and increased MDA levels. Western blotting indicated that CD significantly increased the levels of Atg7, LC3-I/II, attenuated PARP and caspase3. The expression of the Toll-like receptor 4, iNOS and NFkB were decreased in the CD treatment group .

CD improved the acute hepatic IRI by reducing oxidative damage, apoptosis, inflammation and inducing autophagy. These findings suggest that CD is a promising agent against acute IR-induced hepatic damage.

CITATION INFORMATION: Jang H, Lee H, Park C, Eom D, Lee J, Oh M, Yoo J, Rho C. Protective Effect of Crepidiastrum denticulatum Extract Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice. Am J Transplant. 2017;17 (suppl 3).

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