Articles tagged "Xenotransplantation"

2017 American Transplant Congress
Ex-Vivo Mitomycin-C Exposure Reduced Immunogenicity and Prolonged Survival of Islets by Suppressing the Secretion of Multiple Chemotaxis Factors.
N. Sato,¹ T. Anazawa,² A. Kenjyo,¹ T. Kimura,¹ R. Okada,¹ T. Ishigame,¹ Y. Kofunato,¹ J. Watanabe,¹ M. Muto,¹ A. Nishimagi,¹ M. Gotoh,¹ S. Marubashi.¹
¹Department of Surgery, Division of Hepato-Biliary-Pancreatic and Transplant, Fukushima Medical University, Fukushima, Japan; ²Department of Surgery, Graduate School of Midicine, Kyoto University, Kyoto, Japan
Background. Strategies to reduce immunogenicity of islets, and to prevent activation of pro-inflammatory events are essential to successful islet engraftment. We previously demonstrated that ex-vivo…
2017 American Transplant Congress
Porcine Albumin Elaboration During Perfusion of Transgenic Porcine Livers with Human Blood.
A. Cimeno,¹ J. Powell,¹ B. French,¹ D. Ayares,² A. Azimzadeh,¹ R. Pierson III,¹ R. Barth,¹ J. LaMattina.
¹Department of Surgery, University of Maryland, School of Medicine, Baltimore, MD; ²Revivicor, Inc., Blacksburg, VA
PurposeIn addition to immune barriers, molecular incompatibilities between species are predicted to limit pig liver survival in primate xenotransplantation models. How perfusion with human blood…
2017 American Transplant Congress
Xenogeneic Lung Transplantation: Identifying Transgenes and Supportive Drug Treatments to Achieve Long-Term Survival.
L. Burdorf,¹ C. Laird,¹ N. O'Neill,¹ T. Zhang,¹ D. Parsell,¹ I. Tatarov,¹ A. Cimeno,² S. Sendil,¹ K. Thomas,¹ C. Phelps,² D. Ayares,² A. Azimzadeh,¹ R. Pierson III.¹
¹Surgery, University of Maryland, Baltimore, MD; ²Revivicor, Inc., Blacksburg, VA
Purpose: Recent progress in genetic engineering has facilitated generation of pigs expressing various multi-transgene and knock-out combinations. Which transgenes and what targeted drug treatments are…
2017 American Transplant Congress
Recognition of Islet Xenografts In Vivo by Non-Obese Diabetic (NOD) Insulin-Specific CD4 T Cells.
R. Gill, W. Dale, M. Crawford.
Surgery and Immunology, University of Colorado Denver, Aurora, CO
Xenogeneic islet transplantation has been one ongoing goal for treating autoimmune Type 1 diabetes. Autoimmune diabetes involves a T cell response restricted to the host…
2016 American Transplant Congress
Long Term Heterotopic Cardiac Xenograft Survival from Donor Pigs with Six Gene Modifications.
M. Mohiuddin,¹ A. Singh,¹ J. Chan,¹ P. Corcoran,¹ M. Thomas III,² B. Lewis,² D. Ayares,³ K. Horvath.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / RS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburgh, VA.
Introduction: We have recently reported pig xenograft survival of more than 2.5 years in a heterotopic cardiac xenograft model. Some of the animals exhibited local…
2016 American Transplant Congress
Human Leukocyte Antigen-E Expression on Porcine GalTKO.hCD46 Cells Improves Ex-Vivo Xenograft Survival and Attenuates Injury.
C. Laird,¹ N. Kubicki,¹ L. Burdorf,¹ T. Zhang,¹ X. Chang,¹ G. Braileanu,¹ C. Phelps,² D. Ayares,² A. Azimzadeh,¹ R. Pierson.¹
¹University of Maryland School of Medicine, Baltimore, MD; ²Revivicor, Inc, Blacksburg, VA.
Purpose:Lung xenografts are subject to injury mechanisms associated with the sequestration of human cells, including natural killer (NK) cells. To control NK cell associated injury,…
2016 American Transplant Congress
Long-Term Islet Xenograft Survival Requires Effective Control of Early Xenogeneic IL-17 Response and Late Xenogeneic IFN-γ Response.
H. Kang,¹ S. Wang,² X. Zhang,³ A. Singh,⁵ L. Zhang,¹ W. Suarez-Pinzon,⁵ S. Miller,⁴ B. Hering,⁵ X. Luo.^1,3,4
¹Medicine, Northwestern University, Chicago, IL; ²Surgery, Tianjin Union Medical Center, Tianjin, China; ³Comprehensive Transplant Center, Northwestern University, Chicago, IL; ⁴Microbiology and Immunology, Northwestern University, Chicago, IL; ⁵Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN.
Introduction: Tolerance induction for xenogeneic islet transplant remains a challenge. We have previously shown that peri-transplant infusion of donor cells treated with 1-ethyl-3-(3'-dimethylaminopropyl)-carbodiimide (ECDI) is…
2016 American Transplant Congress
Successful Human-to-NonHuman Primate Pancreatic Islet Intraportal Xenotransplantation Using a Human Allo-Based Immunosuppression Regimen and Its Potential Pre-Clinical Application.
J. McGarrigle,¹ M. Bochenek,¹ E. Marchese,¹ A. Khan,¹ D. Gutierrez,^1,2 A. Mikalauskaite,¹ B. McCracken,¹ M. Reedy,¹ M. Omami,¹ C.-C. Yeh,¹ M. Spaggiari,¹ M. Nourmohammadzadeh,^1,2 D. Isa,¹ S. Ghani,¹ L.-J. Wang,¹ J. Mendoza-Elias,^1,2 Y. Wang,¹ J. Oberholzer.^1,2
¹Surgery, University of Illinois at Chicago, Chicago; ²Bioengineering, University of Illinois at Chicago, Chicago.
Intraportal pancreatic islet cell transplantation is a successful treatment for type-1 diabetes (T1DM) in humans. Unfortunately, the lack of donor pancreatic organs is a major…
2016 American Transplant Congress
Multiplex Profiling of Plasma Proteins After Xenogenic Lung Exposure.
A. Azimzadeh,¹ N. Cheng,¹ X. Cheng,¹ E. Schwartz,¹ L. Burdorf,¹ S. Dahi,¹ N. Kubicki,¹ D. Parsell,¹ T. Zhang,¹ D. Cooper,² C. Phelps,³ D. Ayares,³ R. Pierson III.¹
¹Univ. Of Maryland, Baltimore, MD; ²University of Pittsburgh, Pittsburgh, PN; ³Revivicor, Blacksburg, VA.
Background: Porcine lungs exhibit injury by human blood and are only partially protected in GalTKO organs with additional human membrane cofactor protein (hCD46). Residual injury…
2016 American Transplant Congress
Human PDL1⁺ SLA-DR^–Swine Vascular Endothelial Cells Suppress Pig-Human PBMC Xeno-MLR(Mixed Lymphocyte Reaction) by Inducing Human Regulatory IL-10⁺Tr1(Type 1 Regulatory T Cells) Subset for Suppression of Xenogeneic Reactions.
Q. Shao, Q. Diag.
The Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, China.
Type 1 regulatory T cells(Tr1) are active in transplantation tolerance. The Tr1 cells are inducible in our lab from human CD4 T cells (hCD4 T)…