Date: Sunday, June 12, 2016
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Halls C&D
Type 1 regulatory T cells(Tr1) are active in transplantation tolerance. The Tr1 cells are inducible in our lab from human CD4 T cells (hCD4 T) by hPDL1-overexpressed accessory A431 cells(human epidermoid cell line) in a way that at least one DR-DQ haplotype is shared between the hCD4 T and the PDL1+ A431. When A431 were substituted with PDL1-transfected porcine iliac artery endothelial cells(PIECs), hCD4 Tcould still be primed to differentiate into human Tr1 cells if the expression of swine SLA-DR, instead of SLA-DQ, on the PIECs was blocked with mAb. The activated Tr1 were potent for inducing suppression of xenogeneic mixed lymphocyte-endothelial cell response (xeno-MLER) by secreting IL-10 and TGF-beta. The PIECs were thus further cotransfected with mutated class II transactivator (mCIITA) and PDL1 genes to guarantee the cells with capabilities of stopping SLA-DR expression and inducing Tr1. Again,the resultant PDL1+SLA-DR–PIECs functioned well for human Tr1 generation. Repeat stimulating hCD4 T cells with the mCIITA/PDL1-cotransfected PIECs left a CD4+CD25–Foxp3–IFN-γR+IL-10R+CD45RBlow Tr1 population at purity of 93.6% (3.8% in control), which down-regulated the xeno-MLER in a dose-dependent manner. It implicates that the hPDL1+SLA-DR–PIECs are hopeful to be utilized as a cellular reagent for actively inducing regulatory Tr1 cells even in histoincompatible conditions.
CITATION INFORMATION: Shao Q, Diag Q. Human PDL1+ SLA-DR– Swine Vascular Endothelial Cells Suppress Pig-Human PBMC Xeno-MLR(Mixed Lymphocyte Reaction) by Inducing Human Regulatory IL-10+Tr1(Type 1 Regulatory T Cells) Subset for Suppression of Xenogeneic Reactions. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:Shao Q, Diag Q. Human PDL1+ SLA-DR– Swine Vascular Endothelial Cells Suppress Pig-Human PBMC Xeno-MLR(Mixed Lymphocyte Reaction) by Inducing Human Regulatory IL-10+Tr1(Type 1 Regulatory T Cells) Subset for Suppression of Xenogeneic Reactions. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/human-pdl1-sla-dr-swine-vascular-endothelial-cells-suppress-pig-human-pbmc-xeno-mlrmixed-lymphocyte-reaction-by-inducing-human-regulatory-il-10tr1type-1-regulatory-t-cells-subset-for-suppressio/. Accessed June 4, 2020.
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