Articles tagged "Xenotransplantation"

2017 American Transplant Congress
Ex-Vivo Mitomycin-C Exposure Reduced Immunogenicity and Prolonged Survival of Islets by Suppressing the Secretion of Multiple Chemotaxis Factors.
N. Sato,¹ T. Anazawa,² A. Kenjyo,¹ T. Kimura,¹ R. Okada,¹ T. Ishigame,¹ Y. Kofunato,¹ J. Watanabe,¹ M. Muto,¹ A. Nishimagi,¹ M. Gotoh,¹ S. Marubashi.¹
¹Department of Surgery, Division of Hepato-Biliary-Pancreatic and Transplant, Fukushima Medical University, Fukushima, Japan; ²Department of Surgery, Graduate School of Midicine, Kyoto University, Kyoto, Japan
Background. Strategies to reduce immunogenicity of islets, and to prevent activation of pro-inflammatory events are essential to successful islet engraftment. We previously demonstrated that ex-vivo…
2017 American Transplant Congress
Porcine Albumin Elaboration During Perfusion of Transgenic Porcine Livers with Human Blood.
A. Cimeno,¹ J. Powell,¹ B. French,¹ D. Ayares,² A. Azimzadeh,¹ R. Pierson III,¹ R. Barth,¹ J. LaMattina.
¹Department of Surgery, University of Maryland, School of Medicine, Baltimore, MD; ²Revivicor, Inc., Blacksburg, VA
PurposeIn addition to immune barriers, molecular incompatibilities between species are predicted to limit pig liver survival in primate xenotransplantation models. How perfusion with human blood…
2017 American Transplant Congress
Xenogeneic Lung Transplantation: Identifying Transgenes and Supportive Drug Treatments to Achieve Long-Term Survival.
L. Burdorf,¹ C. Laird,¹ N. O'Neill,¹ T. Zhang,¹ D. Parsell,¹ I. Tatarov,¹ A. Cimeno,² S. Sendil,¹ K. Thomas,¹ C. Phelps,² D. Ayares,² A. Azimzadeh,¹ R. Pierson III.¹
¹Surgery, University of Maryland, Baltimore, MD; ²Revivicor, Inc., Blacksburg, VA
Purpose: Recent progress in genetic engineering has facilitated generation of pigs expressing various multi-transgene and knock-out combinations. Which transgenes and what targeted drug treatments are…
2017 American Transplant Congress
Recognition of Islet Xenografts In Vivo by Non-Obese Diabetic (NOD) Insulin-Specific CD4 T Cells.
R. Gill, W. Dale, M. Crawford.
Surgery and Immunology, University of Colorado Denver, Aurora, CO
Xenogeneic islet transplantation has been one ongoing goal for treating autoimmune Type 1 diabetes. Autoimmune diabetes involves a T cell response restricted to the host…
2016 American Transplant Congress
Comparison of Early Engraftment Immunobiology of Xenoislets and Alloislets in a Dual Transplant Model.
K. Samy,¹ B. Martin,² M. Song,¹ B. Collins,¹ E. Gall,¹ J. Cano,² A. Farris,² F. Leopardi,¹ A. Kirk.^1,2
¹Department of Surgery, Duke University Medical Center, Durham, NC; ²Emory University, Atlanta, GA.
Purpose: Successful implementation of neonatal porcine islet (NPI) xenotransplantation requires an understanding of the xenospecific immune elements influencing engraftment. To elucidate these early xenospecific processes,…
2016 American Transplant Congress
Late Renal Xenograft Failure Is Antibody-Mediated: Description of the Longest-Reported Survival in Pig-to-Primate Renal Xenotransplantation.
L. Higginbotham,¹ S. Kim,¹ D. Mathews,¹ A. Stephenson,¹ C. Breeden,¹ C. Larsen,¹ M. Ford,¹ K. Newell,¹ A. Tector,² A. Adams.¹
¹Emory Transplant Center, Emory University Hospital, Atlanta, GA; ²Indiana University School of Medicine, Indianapolis, IN.
Introduction. Xenotransplantation has the potential to alleviate the organ supply shortage, yet pig-to-primate renal xenotransplant has met limited success due to natural preformed anti-pig antibody.…
2016 American Transplant Congress
Costimulation Blockade by Anti CD40 Antibody Prevents Humoral and Cellular Rejection of Heterotopic Pig Cardiac Xenograft.
A. Singh,¹ J. Chan,¹ P. Corcoran,¹ B. Lewis,² M. Thomas,² D. Ayares,³ K. Horvath,¹ M. Mohiuddin.¹
¹CSRP/NHLBI, NIH, Bethesda, MD; ²DVR/ORS, NIH, Bethesda, MD; ³Revivicor Inc., Blacksburg, VA.
BackgroundXenotransplantation (XTx) involves the participation of innate immune system with natural antibody producing B cells, NK cells, macrophages and complement as well as adaptive immune…
2016 American Transplant Congress
Inclusion of Human Tissue Factor Pathway Inhibitor or Thrombomodulin Transgenes Attenuates Critical Consumptive Thrombocytopenia in Cardiac Xenotransplantation.
J. Chan,¹ A. Singh,¹ P. Corcoran,¹ M. Thomas III,² B. Lewis,² D. Ayares,³ K. Horvath,¹ M. Mohiuddin.¹
¹Cardiothoracic Surgery Research Program/National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD; ²Division of Veterinary Resources, National Institutes of Health, Bethesda, MD; ³Revivicor Inc., Blacksburg, VA.
ObjectiveA major obstacle in the success of cardiac xenotransplantation has been the development of significant consumptive thrombocytopenia, frequently limiting recipient survival. Advances in genetic engineering…
2016 American Transplant Congress
The Impact of Sequential Gene Modifications on Cardiac Xenograft Survival.
K. Horvath,¹ A. Singh,¹ J. Chan,¹ M. Thomas,² B. Lewis,² D. Ayares,³ P. Corcoran,¹ M. Mohiuddin.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / ORS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburg, VA.
Introduction: One of the major advances in xenotransplantation is the ability to modify donor pig genetics. Using rapidly evolving techniques of pig cloning and gene…
2016 American Transplant Congress
In Vivo and In Vitro Characterization of 1.5 mm Ba²⁺-Alginate Macro-Encapsulated Cynomolgus Monkey Islets.
M. Bochenek,¹ J. McGarrigle,¹ J. Mendoza-Elias,¹ E. Marchese,¹ M. Nourmohammadzadeh,¹ Y. Wang,¹ A. Khan,¹ M. Omami,¹ C.-C. Yeh,¹ I. Lacik,² B. Strand,³ J. Oberholzer.¹
¹Transplant Surgery, University of Iliinois at Chicago, Chicago, IL; ²Biomaterials Research, Polymer Institute of the Slovak Academy of Sciences, Bratislava, Slovakia (Slovak Republic); ³Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway.
Introduction:Micro-encapsulation of islets for the treatment of Type I diabetes has shown limited efficacy in small-scale clinical trials. The lack of success may be related…