Articles tagged "Non-invasive diagnosis"

2021 American Transplant Congress
A Noninvasive Diagnostic Approach for Molecular Monitoring of Face Transplant Recipients
C. Snopkowski¹, P. Rabbani², H. Yang¹, Z. Berman², G. Diep², C. Li¹, T. Muthukumar¹, R. Ding¹, D. Ceradini², M. Suthanthiran¹
¹Weill Cornell Medical Institute, Department of Medicine, Division of Nephrology, NY, NY, ²NYU Langone Health, Hansjörg Wyss Department of Plastic Surgery, NY, NY
*Purpose: Skin biopsies are currently the gold standard for evaluating the inflammatory status of face allografts. However, “tape stripping” is emerging as a noninvasive technique…
2021 American Transplant Congress
Donor-Derived Cell-Free DNA as a Surrogate Marker for “Allograft Quiescence” After Kidney Transplantation
J. S. Bromberg¹, L. Bu², G. Gupta³, I. Moinuddin³, S. Anand⁴, A. Pai⁵, T. Alhamad⁶, V. Bowers⁷, S. Ghosh⁸, W. Tian⁸, E. Stites⁹
¹University of Maryland School of Medicine, Baltimore, MD, ²University of Minnesota, Minneapolis, MN, ³Virginia Commonwealth University, Richmond, VA, ⁴Intermountain Medical Center, Murray, UT, ⁵University of Texas McGovern Medical School, Houston, TX, ⁶Washington University in St. Louis, St. Louis, MO, ⁷Tampa General Hospital, Tampa, FL, ⁸CareDx, Brisbane, CA, ⁹University of Colorado, Aurora, CO
*Purpose: Donor-derived cell-free DNA (dd-cfDNA; AlloSure®) and the association with active rejection (AR) was described by Bloom et al, however subsequent insights have suggested this…
2021 American Transplant Congress
A Multimodal Interrogation of Human Renal Allografts
D. Dadhania¹, R. Ding¹, H. Xu², C. Lee¹, C. Snopkowski¹, T. Salinas¹, T. Muthukumar¹, J. Lee¹, R. Woodward², M. Grskovic², S. Dholakia², M. Suthanthiran¹
¹Weill Cornell Medicine - NYPH, New York, NY, ²CareDx, New York, NY
*Purpose: Development and validation of noninvasive biomarkers of kidney allograft rejection should improve transplant outcomes. CTOT-04 study validated urinary cell mRNA signature (CTOT04-Sig) for T-cell…
2021 American Transplant Congress
Association of Donor-Derived Cell-Free DNA with Severity of Interstitial Fibrosis and Cortical Atrophy Lesions Following Kidney Transplantation
L. Bu¹, E. Stites², V. Bowers³, T. Alhamad⁴, J. S. Bromberg⁵, G. Gupta⁶, I. Moinuddin⁶, S. Ghosh⁷, W. Tian⁷, A. Pai⁸, S. Anand⁹
¹University of Minnesota, Minneapolis, MN, ²University of Colorado, Aurora, CO, ³Tampa General Hospital, Tampa, FL, ⁴Washington University in St. Louis, St. Louis, MO, ⁵University of Maryland School of Medicine, Baltimore, MD, ⁶Virginia Commonwealth University, Richmond, VA, ⁷CareDx, Brisbane, CA, ⁸University of Texas McGovern Medical School, Houston, TX, ⁹Intermountain Medical Center, Murray, UT
*Purpose: The most common cause of kidney transplant (KT) failure is the poorly characterized histopathologic entity “interstitial fibrosis and tubular atrophy” (IFTA). There are no…
2021 American Transplant Congress
High Levels of Donor-Derived Cell-Free DNA Predict EGFR Decline After Kidney Transplantation
T. Alhamad¹, V. Bowers², E. Stites³, S. Anand⁴, J. S. Bromberg⁵, H. Murad¹, G. Gupta⁶, I. Moinuddin⁶, L. Bu⁷, S. Ghosh⁸, J. Zeng⁸, A. Pai⁹
¹Washington University in St. Louis, St. Louis, MO, ²Tampa General Hospital, Tampa, FL, ³University of Colorado, Aurora, CO, ⁴Intermountain Medical Center, Murray, UT, ⁵University of Maryland School of Medicine, Baltimore, MD, ⁶Virginia Commonwealth University, Richmond, VA, ⁷University of Minnesota, Minneapolis, MN, ⁸CareDx, Brisbane, CA, ⁹University of Texas McGovern Medical School, Houston, TX
*Purpose: Persistent, low-grade inflammation is associated with decline in eGFR. Cross-sectional studies have demonstrated a consistent association between circulating inflammatory markers and kidney function. Our…
2021 American Transplant Congress
Benign Pneumatosis Intestinalis with Pneumoperitoneum in the Immunocompromised Patient: Three Cases
A. Mohamed, K. Delvecchio, T. Kitajima, M. Rizzari, K. Collins, A. Yoshida, S. Nagai, J. Denny, M. Abouljoud
Department of Transplant and Hepatobiliary Surgery, Henry Ford Hospital, Detroit, MI
*Purpose: Isolated pneumatosis intestinalis after solid organ transplantation, as well as its surgical management in appropriate clinical context, is a well described entity. The management…
2021 American Transplant Congress
Assessment of Donor-Derived Cell-Free DNA Performance Characteristics Across the Spectrum of TCMR and ABMR After Kidney Transplant
L. Bu¹, S. Anand², A. Pai³, J. S. Bromberg⁴, T. Alhamad⁵, V. Bowers⁶, I. Moinuddin⁷, S. Ghosh⁸, W. Tian⁸, E. Stites⁹, G. Gupta⁷
¹University of Minnesota, Minneapolis, MN, ²Intermountain Medical Center, Murray, UT, ³University of Texas McGovern Medical School, Houston, TX, ⁴University of Maryland School of Medicine, Baltimore, MD, ⁵Washington University in St. Louis, St. Louis, MO, ⁶Tampa General Hospital, Tampa, FL, ⁷Virginia Commonwealth University, Richmond, VA, ⁸CareDx, Brisbane, CA, ⁹University of Colorado, Aurora, CO
*Purpose: Different donor-derived cell-free DNA (dd-cfDNA) thresholds have been reported in association with varying TCMR, ABMR and “Borderline” diagnoses. Interpretation of dd-cfDNA levels spanning active…
2021 American Transplant Congress
Kidney Transplant Rejection Can Be Diagnosed or Even Predicted by Tracking Donor Reactive T Cell Clones in Post-transplant Samples
Y. Sambandam¹, M. Kandpal¹, J. He¹, X. Huang¹, T. S. Taylor¹, A. A. Shetty², J. M. Mathew¹, J. R. Leventhal¹
¹Surgery-Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL, ²Medicine, Nephrology Division, Northwestern University Feinberg School of Medicine, Chicago, IL
*Purpose: To test if kidney transplant (KTx) rejection can be diagnosed by monitoring for donor reactive T-cell (DRTC) clones in post-transplant (post-tx) biopsy and non-invasively…
2021 American Transplant Congress
Donor-Derived Cell-Free DNA Levels Risk-Stratify Polyoma BK Viremia and Associated Clinical Events After Kidney Transplantation – Preliminary Results from ADMIRAL Study
A. Pai¹, L. Bu², J. S. Bromberg³, G. Gupta⁴, I. Moinuddin⁴, T. Alhamad⁵, V. Bowers⁶, S. Ghosh⁷, W. Tian⁷, E. Stites⁸, S. Anand⁹
¹University of Texas McGovern Medical School, Houston, TX, ²University of Minnesota, Minneapolis, MN, ³University of Maryland School of Medicine, Baltimore, MD, ⁴Virginia Commonwealth University, Richmond, VA, ⁵Washington University in St. Louis, St. Louis, MO, ⁶Tampa General Hospital, Tampa, FL, ⁷CareDx, Brisbane, CA, ⁸University of Colorado, Aurora, CO, ⁹Intermountain Medical Center, Murray, UT
*Purpose: The threshold of 10,000 copies for blood Quant BK PCR has been used as a surrogate for BK nephropathy (BKN) in kidney transplant recipients…
2021 American Transplant Congress
High Donor-Derived Cell-Free DNA Levels Predict Development of De Novo HLA Donor-Specific Antibodies After Kidney Transplantation – Data from the ADMIRAL Study
G. Gupta¹, T. Alhamad², V. Bowers³, I. Moinuddin¹, S. Ghosh⁴, J. Zeng⁴, E. Stites⁵, A. Pai⁶, J. S. Bromberg⁷, S. Anand⁸
¹Virginia Commonwealth University, Richmond, VA, ²Washington University in St. Louis, St. Louis, MO, ³Tampa General Hospital, Tampa, FL, ⁴CareDx, Brisbane, CA, ⁵University of Colorado, Aurora, CO, ⁶University of Texas McGovern Medical School, Houston, TX, ⁷University of Maryland School of Medicine, Baltimore, MD, ⁸Intermountain Medical Center, Murray, UT
*Purpose: Donor-derived cell-free DNA (dd-cfDNA) as a marker of injury can serve to quantify subclinical inflammation and molecular injury. As the release of graft genomic…