Articles tagged "Methodology"

2020 American Transplant Congress
microPOTS: A New Single Cell Proteomic Method for Profiling Human Kidney Transplant Biopsy Tissue
T. Sigdel¹, P. Piehowski², S. Roy¹, J. Liberto¹, A. Schroeder¹, P. Rashmi¹, S. Sur¹, I. Damm¹, W. Qian², M. M. Sarwal¹
¹University of California San Francisco, San Francisco, CA, ²Pacific Northwest National Laboratory, Richland, WA
*Purpose: The purpose of this study was to interrogate acute rejection (AR) in the renal allograft at single cell level. Despite advances in single cell…
2020 American Transplant Congress
The Centers for Medicare and Medicaid Services’ Proposed Metrics for Recertification of Organ Procurement Organizations: Evaluation by the Scientific Registry of Transplant Recipients
J. Snyder¹, D. Musgrove¹, D. Zaun¹, A. Wey¹, N. Salkowski¹, J. Rosendale², A. Israni¹, R. Hirose³, B. Kasiske¹
¹SRTR, Minneapolis, MN, ²UNOS, Richmond, VA, ³Department of Surgery, Univ of California, San Francisco, San Francisco, CA
*Purpose: On December 23, 2019, the US Centers for Medicare and Medicaid Services proposed two new standards that organ procurement organizations (OPOs) must meet for…
2020 American Transplant Congress
Mass-Spectrometry Based Discovery of Tubular Injury Marker in Chronic Kidney Disease
J. Kim¹, D. Han¹, J. Jeong², J. Moon¹, S. Lee³, Y. Kim¹, K. Yoo⁴, D. Kim¹, Y. Kim¹, S. Yang¹
¹Seoul National University Hospital, Seoul, Korea, Republic of, ²Veterans Health Service Medical Center, Seoul, Korea, Republic of, ³Kangwon National University Hospital, Kangwon-do, Korea, Republic of, ⁴Ulsan National University Hospital, Ulsan, Korea, Republic of
*Purpose: Renal tubular injury and interstitial fibrosis are inevitable processes in the progression of chronic kidney disease (CKD). The purpose of this study is to…
2020 American Transplant Congress
Enumeration of Platelets by Flow Cytometry Reveals Human Erythrocyte Fragmentation During Pig Organ Xenoperfusion Studies
Z. Abady¹, K. M. Petitpas¹, L. Burdorf¹, S. G. Pratts¹, B. M. Cerel¹, S. Sendil², M. R. Connolly¹, W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Surgery, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA, ²Surgery, University of Maryland School of Medicine, Baltimore, MD, ³eGenesis, Cambridge, MA
*Purpose: Human or baboon platelet sequestration and activation occurs during pig organ xenograft perfusion, although the mechanism remains poorly understood. To evaluate an apparent “rebound”…
2020 American Transplant Congress
Microarray and RNA-Seq Analysis of Rejection in Formalin-Fixed Paraffin-Embedded (FFPE) Biopsies: Comparing FFPE to Conventionally Stabilized (RNAlater Solution) Biopsies
P. Halloran¹, K. Famulski¹, J. Reeve¹, J. Huang², N. Alexandrov²
¹University of Alberta, Edmonton, AB, Canada, ²R&D Transplant Diagnostics Division, Thermo Fisher Scientific, Los Angeles, CA
*Purpose: Molecular understanding of organ transplant biopsies requires genome-wide measurements and molecular classifiers. To assess potential of performing molecular diagnostics on formalin-fixed paraffin-embedded (FFPE) biopsies,…
2020 American Transplant Congress
The State of Interventional Clinical Trials in Solid Organ Transplant: Insights from a Systematic Analysis of Clinicaltrials.gov
A. C. Swaminathan¹, J. Li¹, K. Chiswell¹, W. McManigle¹, I. O’Doherty², W. Fitzsimmons³, J. L. Todd¹, S. Tibbs⁴, S. M. Palmer¹
¹Duke Clinical Research Institute, Durham, NC, ²Critical Path Institute, Tuscan, AZ, ³Fitzsimmons LLC, Chicago, IL, ⁴Duke University, Durham, NC
*Purpose: The approach to immunosuppression after solid organ transplant has remained relatively unchanged for almost 20 years, despite the high prevalence of immunosuppression-related complications and…
2020 American Transplant Congress
Challenges in Examining Transplant Outcomes in Patients with Fontan-Associated Liver Disease
R. T. Fischer¹, M. H. Kim², A. Nguyen³, S. R. Kumar³, J. Bucuvalas⁴, E. F. Glynn¹, M. A. Hoffman¹, J. Emamaullee³
¹Children's Mercy Hospital, Kansas City, MO, ²Children's Hospital-Los Angeles, Los Angeles, CA, ³University of Southern California, Los Angeles, CA, ⁴Mount Sinai Kravis Children's Hospital, New York, NY
*Purpose: The Fontan procedure has allowed patients born with single ventricle physiology to survive into adulthood. It is projected that the mean age of Fontan…
2020 American Transplant Congress
Patient-Informed Design of an Educational Website on Living Kidney Donation
M. Keller¹, R. Seibert¹, T. Feeley², L. Kayler¹
¹Erie County Medical Center, Buffalo, NY, ²University at Buffalo, SUNY, Buffalo, NY
*Purpose: Web-based education may be a powerful tool to support kidney patients’ learning about living kidney donation (LKD); however, websites that are not user-friendly can…
2019 American Transplant Congress
Proteomics Reveals Molecular Window and Metabolism of Normothermically Perfused Human Kidneys
A. Weissenbacher¹, H. Huang², A. Thorne³, L. M. Lo Faro³, J. Hunter³, R. Ploeg³, C. C. Coussios⁴, P. J. Friend³, B. Kessler⁵
¹Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innsbruck, Austria, ²Nuffield Department o Medicine, Target Discovery Institute, University of Oxford, Oxford, United Kingdom, ³Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom, ⁴Oxford Institute of Biomedical Engineering, University of Oxford, Oxford, United Kingdom, ⁵Nuffield Department o Medicine, Target Discovery Instituteiscovery Institute, University of Oxford, Oxford, United Kingdom
*Purpose: We have shown that 24h normothermic kidney perfusion (NKP) with urine recirculation (URC) is superior to non URC for maintaining homeostasis previously. However, the…
2019 American Transplant Congress
Modeling Patients as Dynamical Systems: Evaluating the Efficiency of Kidney Transplantation through Multi-Stage Data Envelopment Analysis
F. J. Santos Arteaga¹, D. Di Caprio², D. Cucchiari³, J. M. Campistol³, F. Oppenheimer³, F. Diekmann³, I. Revuelta³
¹Faculty of Economics and Management, Free University of Bolzano, Bolzano, Italy, ²Department of Mathematics and Statistics, York University, Toronto, ON, Canada, ³Department of Nephrology and Kidney Transplant, Hospital Clinic, Barcelona, Spain
*Purpose: The occurrence of kidney graft loss is determined by a large set of variables interacting across the different stages of the transplantation process. This…