Articles tagged "Image analysis"

2019 American Transplant Congress
Near-Infrared Fluorescence Imaging with ZW800-1 Dye to Assess Donor Kidneys While on Ex-Vivo Normothermic Machine Perfusion
A. S. Arykbaeva¹, K. M. Rozenberg², M. L. Lo Faro², J. Hunter², J. B. Doppenberg¹, A. R. Valentijn³, A. G. Terwisscha van Scheltinga³, H. Putter⁴, I. P. Alwayn¹, J. V. Frangioni⁵, J. Burggraaf⁶, A. L. Vahrmeijer¹, R. J. Ploeg², V. A. Huurman¹
¹Department of Surgery, Leiden University Medical Center, Leiden, Netherlands, ²Nuffield Department of Surgical Sciences, Oxford, United Kingdom, ³Department of Clinical Pharmacy and Toxicology, Leiden University Medical Center, Leiden, Netherlands, ⁴Department of Biomedical Data Sciences, Leiden University Medical Center, Leiden, Netherlands, ⁵Curadel, LCC, Marlborough, MA, ⁶Centre for Human Drug Research, Leiden, Netherlands
*Purpose: At present, more older and higher risk donor kidneys are accepted resulting in increased uncertainty about quality. This may result in unjustified discard of…
2019 American Transplant Congress
Alterations in Brain Metabolites before and after Kidney Transplant
A. Gupta¹, P. Lee², R. Lepping³, A. Jurgensen¹, J. Klein¹, P. Adany⁴, D. Cibrik¹, J. Burns⁵, W. Brooks³, I. Choi⁶
¹Division of Nephrology, The Kidney Institute, University of Kansas Medical Center, Kansas City, KS, ²Hoglund Brain Imaging Center, Department of Molecular & Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, ³Hoglund Brain Imaging Center, Alzheimer’s Disease Center,, University of Kansas Medical Center, Kansas City, KS, ⁴Hoglund Brain Imaging Center, University of Kansas Medical Center, Kansas City, KS, ⁵Alzheimer’s Disease Center, Department of Neurology, University of Kansas Medical Center, Kansas City, KS, ⁶Hoglund Brain Imaging Center, Department of Neurology, University of Kansas Medical Center, Kansas City, KS
*Purpose: End stage renal disease (ESRD) is associated with accumulation of uremic metabolites and poor cognitive performance. Alterations in brain metabolites are associated with cognitive…
2019 American Transplant Congress
Hepatic Artery Anastomosis Post-Operative Anatomy Influences Doppler Ultrasound Systolic Acceleration Time after Liver Transplantation
R. A. Choudhury, A. Yule, K. Conzen, E. A. Pomfret, T. L. Nydam
Transplant Surgery, University of Colorado, Aurora, CO
*Purpose: Prolongation of the systolic acceleration time (SAT) and decreased resistive index (R/I) of the proper hepatic artery following liver transplant have been associated with…
2019 American Transplant Congress
Contrast-Enhanced Ultrasonography Predicts Insulin Secretion Ability at 1 Year after Pancreas Transplantation
N. Aida¹, T. Kenmochi¹, T. Ito¹, K. Kurihara¹, A. Kawai¹, T. Nishikawa², I. Hiratsuka³, M. Shibata³, A. Suzuki³, M. Hasegawa⁴, M. Kusaka⁵, M. Hoshinaga⁵
¹Transplantation and Regenerative Medicine, Fujita Health University, Toyoake, Japan, ²Liver, Biliary Tract and Pancreas Diseases, Fujita Health University, Toyoake, Japan, ³Endocrinology and Metabolism, Fujita Health University, Toyoake, Japan, ⁴Nephrology, Fujita Health University, Toyoake, Japan, ⁵Urology, Fujita Health University, Toyoake, Japan
*Purpose: We have previously reported that contrast enhanced ultrasonography (CEUS) is useful to evaluate the graft circulation and to predict the endocrine function of the…
2019 American Transplant Congress
The Important Roles of Dendritic Cells in Transplantation
T. Ueno¹, M. M. McGrath¹, M. Y. Yeung¹, S. Cai¹, Y. Ueno¹, T. Yokoyama², Y. Kihara², O. Konno², Y. Nakamura², H. Iwamoto², A. K. Chandraker¹
¹Medicine, Renal Division, Brigham and Women's Hospital, Transplantation Research Center, Boston, MA, ²Kidney Transplantation Surgery, Tokyo Medical University, Hachioji Medical Center, Tokyo, Japan
*Purpose: The interplay between dendritic cells (DCs) and T cells plays a central role in the process of allograft rejection. Upon antigen exposure, positive costimulatory…
2019 American Transplant Congress
In Vivo/In Situ Behaviour of Allogeneic Spleen versus Bone Marrow Cells in Wild-Type Neonatal Mice: Insights into Robust Transplant Tolerance Induction
R. A. Bascom, K. Tao, L. J. West
Pediatrics, University of Alberta, Edmonton, AB, Canada
*Purpose: Comparing the behaviour of fully allogeneic spleen (SC) vs bone marrow cells (BMC) in non-conditioned neonatal mice is important for maximizing synergistic interactions between…
2019 American Transplant Congress
Digital Imaging Software vs. the “Eyeball” Method in Quantifying Steatosis in a Liver Biopsy
J. Long¹, J. Motter¹, S. Besharati¹, S. Jerman², R. Anders¹, A. Ortiz², D. Bowman², T. Dunn³, D. Lee⁴, A. Massie¹, R. Barth⁵, B. Philosophe¹
¹Johns Hopkins, Baltimore, MD, ²Indica Labs, Corrales, NM, ³UPenn, Philadelphia, PA, ⁴Mayo Clinic, Jacksonville, FL, ⁵University of MD, Baltimore, MD
*Purpose: The use of steatotic livers is controversial, but may represent an underutilized resource to increase the donor pool. Although
2019 American Transplant Congress
Ex Vivo Normothermic Perfusion Of Discarded Human Kidneys Reveals Cold-storage-induced Renal Fibrinogen And Provides A Platform For Therapeutic Repair
J. DiRito¹, S. A. Hosgood², M. Reschke³, M. L. Nicholson², G. T. Tietjen⁴
¹Surgery, Yale University & University of Cambridge, New Haven, CT, ²Surgery, University of Cambridge, Cambridge, United Kingdom, ³Biomedical Engineering, Yale University, New Haven, CT, ⁴Surgery - Transplantation, Yale University, New Haven, CT
*Purpose: The purpose of this study was to: 1) determine the cause of microvascular obstructions that arise during ex vivo normothermic perfusion (NMP) of human…
2019 American Transplant Congress
Indocyanine Green Fluorescence Imaging during Normothermic Ex Vivo Liver Perfusion for the Assessment of Hepatocyte, Biliary, and Vascular Function
T. Goto, I. Linares, S. Ganesh, L. Mazilescu, P. Urbanellis, N. Selzner, M. Selzner
Multi-Organ Transplant Program, Department of Surgery, Toronto General Hospital, Toronto, ON, Canada
*Purpose: Normothermic Ex Vivo Liver Perfusion (NEVLP) is a novel preservation for the storage and assessment of marginal grafts, such as Donation after Cardio-circulatory Death…
2018 American Transplant Congress
In-Situ Tissue-Based Gene Expression Profiling is a Reliable Method to Detect Gene Transcripts in Human Kidney Transplant Biopsies
D. Dobi,¹ J. Henrik,² T. Sigdel,² A. Chen,¹ Z. Laszik.¹
¹Department of Pathology, University of California, San Francisco, San Francisco, CA; ²Department of Surgery, University of California, San Francisco, San Francisco, CA.
Purpose. Validation studies that would enable the clinical application of quantitative in situ tissue-based gene expression profiling in renal transplantation are lacking. In this study,…