Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall C & D
*Purpose: The interplay between dendritic cells (DCs) and T cells plays a central role in the process of allograft rejection. Upon antigen exposure, positive costimulatory signals provided by DCs lead to T cell activation, while negative signals downmodulate the T cell response. The net balance of these signals ultimately determines allograft fate. In these experiments, our research is aimed at understanding of mechanisms of “the interplay”.
*Methods: We have now adapted the use of intravital microscopy (IVM) to track EYFP DC motility, morphology, and cell density in real-time 1).
*Results: Our preliminary data using cardiac transplant model showed that 1) dDC have already been activated during ischemic time (morphological changes), 2) both injected CD4+T cells and CD8+T cells (5millions on Day 0) were detected at graft, 3) and donor DC (EGFP) has already started cross-talking with CD4+T cells (red) right after reperfusion (Figure). 3-D pathological images also confirmed its finding.
*Conclusions: As we have reported the important roles of DCs in transplantation 2), this proposal leads us to provide to investigate the role of donor dendritic cell-T cell interaction in graft rejection versus tolerance utilizing “state-of-the-art” imaging technologies.
References: 1: Ueno T, et al. Transplant Int. 2016, 2: Batal I, et al. JASN 2015
To cite this abstract in AMA style:Ueno T, McGrath MM, Yeung MY, Cai S, Ueno Y, Yokoyama T, Kihara Y, Konno O, Nakamura Y, Iwamoto H, Chandraker AK. The Important Roles of Dendritic Cells in Transplantation [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/the-important-roles-of-dendritic-cells-in-transplantation/. Accessed July 13, 2020.
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