Articles tagged "Genomics"

2021 American Transplant Congress
Trugraf Gene Expression Testing Can Discriminate Rejection in the Setting of Acute Kidney Injury
N. Singh¹, M. S. Naseer¹, D. Aultman², H. Shokouh-Amiri², G. Zibari²
¹Nephrology, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA, ²Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA
*Purpose: The TruGraf kidney blood gene expression test is a well-validated non-invasive biomarker to detect immune quiescence thus ruling out silent subclinical rejection in stable…
2021 American Transplant Congress
Comparison of Next Generation Sequencing Donor-derived Cell Free DNA Assay Utilizing Whole Genome Sequencing
N. Singh¹, M. S. Naseer¹, D. Aultman², H. Shokouh-Amiri², G. Zibari²
¹Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA, ²Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA
*Purpose: Circulating donor-derived cell free DNA (dd-cfDNA) is a non-invasive biomarker of kidney allograft injury with a high negative predictive value for ruling out acute…
2021 American Transplant Congress
Use of Microbial Cell Free DNA Sequencing in Solid Organ Transplant Patients
S. Chang, P. Gaynor, A. Multani, O. E. Beaird, M. Carlson, S. Yang, O. Garner, J. Schaenman
University of California, Los Angeles, Los Angeles, CA
*Purpose: Microbial cell free DNA (cfDNA) sequencing has emerged as a new noninvasive tool for the diagnosis of infectious diseases in recent years, but its…
2021 American Transplant Congress
Kidney Transplantation in Patients with Hereditary Kidney Diseases
Y. Caliskan¹, N. Garayeva², A. B. Dirim², S. Safak², S. Mirioglu², H. Yazici², A. Yildiz³, O. A. Oto², E. Demir², Y. Ozluk⁴, A. Turkmen², K. Lentine¹
¹Saint Louis University, Saint Louis, MO, ²Division of Nephrology, Istanbul University, Istanbul, Turkey, ³Division of Nephrology, Uludag University, Bursa, Turkey, ⁴Department of Pathology, Istanbul University, Istanbul, Turkey
*Purpose: Genetic testing is poised to play a larger role in the evaluation and management of both kidney transplant (KTx) recipients and donors. We characterized…
2021 American Transplant Congress
The Metagenomic Landscape of Renal Transplant
A. Johnson¹, G. Karadkhele², C. Larsen²
¹Emory University, Atlanta, GA, ²Surgery, Emory University, Atlanta, GA
*Purpose: The selective targeting of T cells in transplant immunosuppression medications leads to a high rate of viral infections. Different immunosuppression regimens have been shown…
2021 American Transplant Congress
Donor-derived Cell-free DNA Distinguishes Acute Rejection from Other Causes of Graft Injury in Liver Transplant Recipients
J. Levitsky¹, M. Miller², R. Sinha², E. Bixler², A. Al-Turck², J. Weems², M. Altrich², S. Kleiboeker², M. Abecassis³
¹Gastroenterology & Hepatology; Comprehensive Transplant Center, Northwestern University, Chicago, IL, ²Viracor Eurofins, Lees Summit, MO, ³University of Arizona College of Medicine Tucson, Tucson, AZ
*Purpose: The donor derived cell-free DNA (ddcfDNA) has emerged as strong biomarker for allograft injury, where the fraction of ddcfDNA is reported to increase in…
2021 American Transplant Congress
Extreme Phenotype Sampling and Next Generation Sequencing to Identify Genetic Variants Associated with Tacrolimus Metabolism in African American Kidney Transplant Recipients
C. R. Dorr¹, B. Guo², B. Wu², J. Abrahante², D. Schladt³, R. Remmel², W. Guan², A. Muthusamy³, G. Onyeaghala³, N. Pankratz², A. Matas², R. Mannon⁴, W. Oetting², P. Jacobson², A. K. Israni³
¹Hennepin Healthcare Research Institute, Minneapolis, MN, ²University of Minnesota, Minneapolis, MN, ³Nephrology, Hennepin Healthcare Research Institute, Minneapolis, MN, ⁴University of Nebraska Medical Center, Omaha, NE
*Purpose: Tacrolimus (TAC) pharmacogenomics studies identified common genetic variants that alter TAC troughs suggesting strategic dosing for specific populations. Common variants and clinical factors explain…
2021 American Transplant Congress
CRISPR Screens to Map CTLA-4 Regulatory Networks in Primary Human T Cells
O. Shaked¹, J. Freimer¹, J. Pritchard², A. Marson¹
¹UCSF, San Francisco, CA, ²Stanford University, Stanford, CA
*Purpose: For most cell types, the pathways that regulate the levels of critical disease-relevant genes are not known. Recent advances in gene editing have enabled…
2021 American Transplant Congress
MiRNA as Regulator of Cell Death and Inflammatory Response in Hepatic Iri
E. Bardhi¹, T. Rousselle¹, J. McDaniels¹, S. Bontha¹, J. Eason², D. Maluf¹, V. Mas³
¹Surgery, University of Maryland, Baltimore, MD, ²Surgery, James D Eason Transplant Institute, Memphis, TN, ³Surgery, University of Maryland - Division of Surgical Science, Baltimore, MD
*Purpose: Hepatic IRI represents a barrier to the use of available organs. Early graft dysfunction of donor livers is directly related to the severity of…
2020 American Transplant Congress
Chronic Diarrhea in Renal Transplant Recipients: A Metagenomic Study of Gut Microbiota Composition and Functionality
J. C. Swarte¹, R. M. Douwes¹, S. Hu², R. Gacesa², H. J. Harmsen³, R. K. Weersma², S. J. Bakker¹
¹Internal Medicine - Nephrology, University Medical Centre Groningen, Groningen, Netherlands, ²Gastroenterology and Hepatology, University Medical Centre Groningen, Groningen, Netherlands, ³Medical Microbiology, University Medical Centre Groningen, Groningen, Netherlands
*Purpose: Chronic diarrhea is a common problem in renal transplant recipients (RTR) and can lead to a lower quality of life. However, in many patients…