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Articles tagged "Genomics"

  • 2021 American Transplant Congress

    The Metagenomic Landscape of Renal Transplant

    A. Johnson1, G. Karadkhele2, C. Larsen2

    1Emory University, Atlanta, GA, 2Surgery, Emory University, Atlanta, GA

    *Purpose: The selective targeting of T cells in transplant immunosuppression medications leads to a high rate of viral infections. Different immunosuppression regimens have been shown…
  • 2021 American Transplant Congress

    Donor-derived Cell-free DNA Distinguishes Acute Rejection from Other Causes of Graft Injury in Liver Transplant Recipients

    J. Levitsky1, M. Miller2, R. Sinha2, E. Bixler2, A. Al-Turck2, J. Weems2, M. Altrich2, S. Kleiboeker2, M. Abecassis3

    1Gastroenterology & Hepatology; Comprehensive Transplant Center, Northwestern University, Chicago, IL, 2Viracor Eurofins, Lees Summit, MO, 3University of Arizona College of Medicine Tucson, Tucson, AZ

    *Purpose: The donor derived cell-free DNA (ddcfDNA) has emerged as strong biomarker for allograft injury, where the fraction of ddcfDNA is reported to increase in…
  • 2021 American Transplant Congress

    Extreme Phenotype Sampling and Next Generation Sequencing to Identify Genetic Variants Associated with Tacrolimus Metabolism in African American Kidney Transplant Recipients

    C. R. Dorr1, B. Guo2, B. Wu2, J. Abrahante2, D. Schladt3, R. Remmel2, W. Guan2, A. Muthusamy3, G. Onyeaghala3, N. Pankratz2, A. Matas2, R. Mannon4, W. Oetting2, P. Jacobson2, A. K. Israni3

    1Hennepin Healthcare Research Institute, Minneapolis, MN, 2University of Minnesota, Minneapolis, MN, 3Nephrology, Hennepin Healthcare Research Institute, Minneapolis, MN, 4University of Nebraska Medical Center, Omaha, NE

    *Purpose: Tacrolimus (TAC) pharmacogenomics studies identified common genetic variants that alter TAC troughs suggesting strategic dosing for specific populations. Common variants and clinical factors explain…
  • 2021 American Transplant Congress

    CRISPR Screens to Map CTLA-4 Regulatory Networks in Primary Human T Cells

    O. Shaked1, J. Freimer1, J. Pritchard2, A. Marson1

    1UCSF, San Francisco, CA, 2Stanford University, Stanford, CA

    *Purpose: For most cell types, the pathways that regulate the levels of critical disease-relevant genes are not known. Recent advances in gene editing have enabled…
  • 2021 American Transplant Congress

    MiRNA as Regulator of Cell Death and Inflammatory Response in Hepatic Iri

    E. Bardhi1, T. Rousselle1, J. McDaniels1, S. Bontha1, J. Eason2, D. Maluf1, V. Mas3

    1Surgery, University of Maryland, Baltimore, MD, 2Surgery, James D Eason Transplant Institute, Memphis, TN, 3Surgery, University of Maryland - Division of Surgical Science, Baltimore, MD

    *Purpose: Hepatic IRI represents a barrier to the use of available organs. Early graft dysfunction of donor livers is directly related to the severity of…
  • 2021 American Transplant Congress

    GENiE in a Bottle – Renasight Testing in the Kidney Transplant Setting – A Single Center Experience

    Y. A. Qazi1, W. Mon1, N. Ioannou2, M. Smogorzewski1

    1Keck Medical Center at USC, Los Angeles, CA, 2Natera, San Carlos, CA

    *Purpose: Renasight™(Natera), a recently available genetic test provides analysis on 382 genes associated with adult-onset kidney disease, covering clinical categories (cystic, tubular, glomerular, complement-related, congenital…
  • 2021 American Transplant Congress

    Nonadherence Post-kidney Transplant is Associated with Increased Immune Activation Detected by Peripheral Blood Gene Expression Assay

    N. Singh1, A. Palermini1, M. S. Naseer1, T. Le1, S. Tandukar1, D. Aultman2, H. Shokouh-Amiri2, G. Zibari2

    1Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA, 2Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA

    *Purpose: Nonadherence to immunosuppression medications is common post-transplantation. We hypothesize that nonadherence results in a heightened alloimmune response in kidney transplant recipients (KTRs) which in…
  • 2021 American Transplant Congress

    Trugraf Gene Expression Testing Can Discriminate Rejection in the Setting of Acute Kidney Injury

    N. Singh1, M. S. Naseer1, D. Aultman2, H. Shokouh-Amiri2, G. Zibari2

    1Nephrology, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA, 2Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Medical Center, Shreveport, LA

    *Purpose: The TruGraf kidney blood gene expression test is a well-validated non-invasive biomarker to detect immune quiescence thus ruling out silent subclinical rejection in stable…
  • 2021 American Transplant Congress

    Comparison of Next Generation Sequencing Donor-derived Cell Free DNA Assay Utilizing Whole Genome Sequencing

    N. Singh1, M. S. Naseer1, D. Aultman2, H. Shokouh-Amiri2, G. Zibari2

    1Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA, 2Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA

    *Purpose: Circulating donor-derived cell free DNA (dd-cfDNA) is a non-invasive biomarker of kidney allograft injury with a high negative predictive value for ruling out acute…
  • 2020 American Transplant Congress

    Discrepant Subtyping of Blood Type A2 Living Kidney Donors in Paired Kidney Exchange

    L. Warnke1, R. R. Redfield1, K. M. Miller1, M. Cooper2, G. Roll3, C. Freise3, B. Lee3, V. Chipman3, A. Thomas4, D. Leeser5, M. Ronin6, G. Hil6, A. D. Waterman7, D. Mandelbrot1

    1Transplant, UW Health, Madison, WI, 2Transplant, Medstar Georgetown, Washington, DC, 3Transplant, UCSF, San Fransisco, CA, 4Surgery, Johns Hopkins University, Baltimore, MD, 5Transplant, ECU, Greenville, NC, 6NKR, Babylon, NY, 7Nephrology, UCLA; Terasaki Research Institute, Los Angeles, CA

    *Purpose: Utilizing non-A1 donors for O or B recipients with low anti-A titers presents an opportunity to expand living donor kidney transplantation. Since February 2018,…
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