Articles tagged "Genomics"

2020 American Transplant Congress
Discrepant Subtyping of Blood Type A2 Living Kidney Donors in Paired Kidney Exchange
L. Warnke¹, R. R. Redfield¹, K. M. Miller¹, M. Cooper², G. Roll³, C. Freise³, B. Lee³, V. Chipman³, A. Thomas⁴, D. Leeser⁵, M. Ronin⁶, G. Hil⁶, A. D. Waterman⁷, D. Mandelbrot¹
¹Transplant, UW Health, Madison, WI, ²Transplant, Medstar Georgetown, Washington, DC, ³Transplant, UCSF, San Fransisco, CA, ⁴Surgery, Johns Hopkins University, Baltimore, MD, ⁵Transplant, ECU, Greenville, NC, ⁶NKR, Babylon, NY, ⁷Nephrology, UCLA; Terasaki Research Institute, Los Angeles, CA
*Purpose: Utilizing non-A1 donors for O or B recipients with low anti-A titers presents an opportunity to expand living donor kidney transplantation. Since February 2018,…
2020 American Transplant Congress
A Bioinformatics Pipeline for the Identification of Crucial Factors for Transplant Rejection Based on NGS Data
J. Vetter¹, A. Heinzel², C. Aschauer², R. Reindl-Schwaighofer², K. Jelencsics², K. Hu², R. Oberbauer², S. Winkler¹, S. Schaller¹
¹Bioinformatics Research Group, University of Applied Sciences Upper Austria, Hagenberg Campus, Hagenberg im Muehlkreis, Austria, ²Department of Nephrology and Dialysis, Medical University Vienna, Vienna, Austria
*Purpose: T and B cells are crucial parts of the human immune system and play an important role in transplantation. Since the mechanisms leading to…
2020 American Transplant Congress
Identification of Novel Gene Targets Inducing Resistance in Liver Ischemia Reperfusion Injury by CRISPR Screening
C. Kuscu, A. R. Wolen, C. Kuscu, C. Watkins, A. Bajwa, J. D. Eason, D. Maluf, V. Mas
Dept of Surgery, Transplant Research Institute, James D. Eason Transplant Institute, University of Tennessee Health Science Center, Memphis, TN
*Purpose: Liver transplantation (LT) is the best treatment option for patients with end-stage liver disease (ESLD). However, the gap between available donors and recipients in…
2020 American Transplant Congress
Prevalence of Risk Factors for NAFLD among Living Liver Donors
D. A. Axelrod¹, M. Simpson², E. Pomfret³, A. Ehrlich⁴, S. Rwema⁴, R. Chung⁴, F. Gordon⁵
¹Surgery, University of Iowa, Iowa CIty, IA, ²Surgery, Lahey Clinic, Burlington, MA, ³University of Colorado, Denver, CA, ⁴Mass General Hospital, Boston, MA, ⁵Lahey, Burlington, MA
*Purpose: The development of non-alcoholic fatty liver (NAFLD) disease has been strongly associated with the presence of polymorphisms in PNPLA3 rs738409. When compared with wild…
2020 American Transplant Congress
Single Cell Profiling of PBMC Identifies Immune Populations Associated with High Risk of Early Acute Rejection
W. Zhang, S. Farouk, Z. Sun, W. Huang, Z. Yi, J. Fu, C. Wei, M. Fribourg-Casajuana, C. He, P. Cravedi, B. Murphy
Icahn School of Medicine at Mount Sinai, New York, NY
*Purpose: Our previous transcriptomic analysis of pre-transplant peripheral blood revealed a 23-gene set predictive of early acute rejection (EAR) post kidney transplant (JCI Insight, 2019).…
2020 American Transplant Congress
Introduction of Preemptive Pharmacogenomic Testing in Kidney Transplant Recipients: Early Evaluation of the Clinical Impact of Genomic Guided Tacrolimus Dosing
G. Girone¹, V. Do¹, K. Belfield¹, R. Pulk¹, E. Cohen¹, E. Marin²
¹Department of Pharmacy, Yale New Haven Hospital, New Haven, CT, ²Department of Nephrology, Yale School of Medicine, New Haven, CT
*Purpose: Tacrolimus is widely accepted as the mainstay of immunosuppression in solid organ transplant. Inherited differences in CYP3A activity are known to contribute to dosing…
2020 American Transplant Congress
Single-Cell RNA Sequencing to Assess the Effect of IL-6 Receptor Blockade in Kidney Transplantation
A. Zarinsefat, P. Rashmi, A. Da Silva, T. Sigdel, S. Chandran, Q. Tang, J. Ye, F. Vincenti, M. Sarwal
University of California San Francisco, San Francisco, CA
*Purpose: Prospective randomized study of IL-6R blockade with Tocilizumab for treatment of subclinical inflammation (SCI) in renal transplantation, reveals unique profiles of resting and stimulated…
2020 American Transplant Congress
Elucidating the Genetic Background of End Stage Renal Disease of Unknown Aetiology in Patients Listed for Kidney Transplantation
E. Leenen¹, B. Beck², A. Joerres¹, A. Weidemann¹
¹Department of Medicine I - Nephrology, Transplantation & Medical Intensive Care, Medical Center Cologne-Merheim, Cologne, Germany, ²Institute of Human Genetics University Hospital Cologne, Medical Center Cologne-Merheim, Cologne, Germany
*Purpose: The prevalence of end stage renal disease (ESRD) of unknown aetiology in adult patients accounts for almost 20% of all dialysis patients in Europe.…
2020 American Transplant Congress
Identification of Molecular Markers for Liver Cirrhosis (NASH) by Single-Nucleus RNA Sequencing
C. Kuscu¹, A. Mouhammed², C. Kuscu³, A. R. Wolen³, J. D. Eason³, D. Maluf³, V. Mas³
¹Dept of Surgery, Transplant Research Institute, James D. Eason Transplant Institute, University of Tennessee Health Science Center, Memphis, TN, ²Bioinformatics, University of Tennessee Health Science Center, Memphis, TN, ³Department of Surgery, University of Tennessee Health Science Center, Memphis, TN
*Purpose: Non-alcoholic fatty liver disease (NAFLD) is a progressive disease and it constitutes a significant portion of the chronic liver diseases that affected more than…
2020 American Transplant Congress
Kidney Transplantation in Inherited Kidney Diseases
Y. Caliskan¹, H. Yazici², R. Ouseph¹, Y. Ozluk³, H. Randall⁴, E. Demir², F. Savran Oguz⁵, A. Turkmen², M. S. Sever², K. Lentine¹
¹Division of Nephrology, Saint Louis University, Saint Louis, MO, ²Division of Nephrology, Istanbul Faculty of Medicine, Istanbul, Turkey, ³Department of Pathology, Istanbul Faculty of Medicine, Istanbul, Turkey, ⁴Center for Abdominal Transplantation, Saint Louis University, Saint Louis, MO, ⁵Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul, Turkey
*Purpose: We aimed to evaluate the kidney transplant (KTx) recipients with inherited kidney diseases and investigate the causal genetic variants in recipients with undetermined etiology.*Methods:…