Articles tagged "Gene expression"

2020 American Transplant Congress
Lung Mucosal Biopsies Deteriorate with Time Post-Transplant: A Molecular Analysis
M. D. Parkes¹, P. Halloran¹, K. Halloran², &. the INTERLUNG Study Group³
¹Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ²University of Alberta, Edmonton, AB, Canada, ³., ., AB, Canada
*Purpose: Mucosal biopsies from the third airway bifurcation (3BMBs) assessed molecularly can be used to detect rejection (DOI: 10.1111/ajt.15685), and may also provide insights into…
2020 American Transplant Congress
The Role of the Molecular Microscope to Guide Therapy for T-Cell Mediated Rejection in Kidney Transplants
D. Kumar¹, M. Demehin¹, J. Christensen¹, L. Kamal¹, I. Moinuddin¹, M. Levy¹, C. Bhati¹, A. King¹, H. D. Massey¹, P. Halloran², G. Gupta¹
¹Virginia Commonwealth University, Glen Allen, VA, ²ATAGC, Edmonton, AB, Canada
*Purpose: Histologic lesions (I+T) to characterize T-cell mediated rejection (TCMR) can be non-specific. In some cases the inflammation may represent a ‘response to wounding’ rather…
2020 American Transplant Congress
Ion AmpliSeq RNA Expression Panel Evaluated by Next-Generation Sequencing (NGS) Can Detect Genes in Human Formalin-Fixed Paraffin-Embedded Kidney Biopsies to Predict T Cell and Antibody Mediated Rejection in Renal Allografts
P. Ruiz¹, E. Margolles-Clark¹, G. Guerra², A. Mattiazzi², M. Ortigosa-Goggins², G. Ciancio¹, L. Chen¹, G. Burke¹
¹Surgery, University of Miami, Miami, FL, ²University of Miami, Miami, FL
*Purpose: Optimization of effective anti-rejection therapies to prevent allograft rejection and to minimize drug toxicity may be aided by utilizing in situ biomarkers for detection…
2020 American Transplant Congress
Oxidative Stress Induces Cytoprotective Alternative Splicing in Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 in Mouse Liver Ischemia-Reperfusion Injury
K. J. Dery, H. Hirao, K. Kadono, S. Kageyama, H. Kojima, R. W. Busuttil, J. W. Kupiec-Weglinski
Surgery, Dumont-UCLA Transplant Center, UCLA, Los Angeles, CA
*Purpose: We have recently shown that carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) serves as a checkpoint regulator in liver transplant (LT) ischemia-reperfusion injury (IRI);…
2020 American Transplant Congress
Assessment of the Usefulness of Urine Signature to Differentiate Acute Rejection in a Prospective Kidney Transplantation Cohort
J. Seo¹, S. Jung², J. Moon², K. Jeong³, C. Kim⁴, B. Chung⁵, J. Park⁶, Y. Kim⁷, S. Lee²
¹Department of Core Research Laboratory, Medical Science Institute, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Republic of, ²Department of Internal Medicine, Division of Nephrology, Kyung Hee University Hospital at Gangdong, Seoul, Korea, Republic of, ³Department of Internal Medicine, Division of Nephrology, College of Medicine, Kyung Hee University, Seoul, Korea, Republic of, ⁴Department of Internal Medicine, Division of Nephrology, Kyung-pook National University School of Medicine, Daegu, Korea, Republic of, ⁵Department of Internal Medicine, Division of Nephrology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea, Republic of, ⁶Department of Internal Medicine, Sungkyunkwan University, Samsung Hospital, College of Medicine, Seoul, Korea, Republic of, ⁷Department of Internal Medicine, Division of Nephrology, Busan Paik Hospital, College of Medicine, Inje University, Busan, Korea, Republic of
*Purpose: Despite many biomarker studies in the field of transplantation, development and validation of noninvasive molecular assays to diagnose disease and monitor immune activity is…
2020 American Transplant Congress
Unexpected Regulation of Foxp3+ Treg Production, Stability and Function by the Nuclear Co-Repressor, Sin3A
L. M. Christensen¹, T. Akimova², R. Han¹, L. Wang¹, A. Samanta¹, W. W. Hancock²
¹Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, ²Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA
*Purpose: Foxp3+ T-regulatory (Treg) cells are crucial to immune homeostasis and self-tolerance, but the contributions to Tregs of the 4 large, multiprotein nuclear complexes (Sin3,…
2020 American Transplant Congress
Single Cell RNA Sequencing of Murine Kidney Allografts Identifies Macrophage Gene Signature Associated with Acute Rejection
A. Dangi¹, N. Natesh², M. Monal¹, J. Wang³, X. Shen², X. Luo¹
¹Nephrology/Medicine, Duke University, Durham, NC, ²Biomedical Engineering, Duke University, Durham, NC, ³Surgery, Duke University, Durham, NC
*Purpose: Evaluating kidney allografts using single cell RNA sequencing (scRNAseq) can provide a comprehensive qualitative and quantitative gene expression data on thousands of individual cells.…
2020 American Transplant Congress
Use of tCRM to Aid Diagnosis of Allograft Rejection in Kidney Transplantation
A. Zarinsefat¹, J. Liberto¹, I. Damm¹, C. Ramirez², J. Guerra², J. Alberu², M. Sarwal¹
¹University of California San Francisco, San Francisco, CA, ²Instituto Nacional de Ciencias Médicas y Nutrición, Mexico City, Mexico
*Purpose: To apply an unbiased, quantitative, molecular profiling score for graft inflammation using standardized assays, in the context of a prospective clinical trial in kidney…
2020 American Transplant Congress
Human Antigen R (HuR): A New Regulator of Heme Oxygenase-1 Cytoprotection in Mouse and Human Liver Transplantation
K. J. Dery¹, K. Nakamura², K. Kadono¹, H. Hirao¹, S. Kageyama¹, T. Ito¹, H. Kojima¹, F. Kaldas¹, R. W. Busuttil¹, J. W. Kupiec-Weglinski¹
¹Department of Surgery, Division of Liver and Pancreas Transplantation, UCLA, Los Angeles, CA, ²Surgery, Nishi-Kobe Medical Center, Hyogo, Japan
*Purpose: Ischemia-reperfusion injury (IRI) represents a risk factor in liver transplantation (LT). We have shown that overexpression of heme oxygenase 1 (HO-1) mitigates hepatic IRI…
2019 American Transplant Congress
Single Cell Sequencing of Human Kidney Allograft
H. Yang¹, H. Suryawanshi², M. Lagman¹, M. Lubetzky¹, A. Alonso¹, S. Seshan¹, T. Tuschl², M. Suthanthiran¹, T. Muthukumar¹
¹Weill Cornell Medicine, New York, NY, ²Rockefeller University, New York, NY
*Purpose: Transcriptome-based clustering of individual cells within the kidney allograft may help identify cell type-specific injury and has the potential to redefine the current histopathology-based…