Articles tagged "Gene expression"

2020 American Transplant Congress
Evolution of Molecular Lung Transplant Biopsy Assessment: Rejection Can be Detected in Mucosal as Well as Transbronchial Biopsies
M. D. Parkes¹, K. Halloran², P. Halloran², &. the INTERLUNG Study Group³
¹Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ²University of Alberta, Edmonton, AB, Canada, ³., ., AB, Canada
*Purpose: We recently developed a Molecular Microscope® (MMDx) rejection assessment platform for mucosal biopsies from the third airway bifurcation (3BMB) and transbronchial biopsies (TBB) from…
2020 American Transplant Congress
Single-Cell RNA Sequencing Reveals Gene Expression Profiling of Ischemia Reperfusion Injury
L. Wang, S. He, J. Xu, Z. Guo, X. He
Organ Transplant Center, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
*Purpose: Ischemia-reperfusion injury (IRI) during liver transplantation is an important factor that leads to graft dysfunction and even recipient death. This study aims to explore…
2020 American Transplant Congress
Evolution of Molecular MMDx Scores after Kidney Transplantationin Different Donor Categories
P. Hruba¹, K. S. Madill-Thomsen², M. Mackova², J. Maluskova³, E. Honsova³, J. Slatinska⁴, P. F. Halloran², O. Viklicky⁴
¹Transplant Laboratory, IKEM, Prague, Czech Republic, ²ATAGC, University of Alberta, Edmonton, AB, Canada, ³Department of Pathology, IKEM, Prague, Czech Republic, ⁴Department of Nephrology, IKEM, Prague, Czech Republic
*Purpose: The outcome of kidney transplantation is highly affected by donor category. Little is known about molecular processes which may underline this phenomenon.*Methods: Using Molecular…
2020 American Transplant Congress
Coagulation and Cd46 Transgene Modification in Neonatal Porcine Islet Xenotransplantation
M. Song, Z. Fitch, K. Samy, Q. Gao, R. P. Davis, F. Leopard, R. Schmitz, N. Huffman, G. Devi, B. Collins, A. D. Kirk
Surgery, Duke University, Durham, NC
*Purpose: Vascular thrombosis is a potential cause for early intraportal xeno-islet loss. To better understand the relationship between coagulation and inflammation in early xeno-islet transplantation,…
2020 American Transplant Congress
To Donate or Not to Donate? The Balancing Act of Apol-1 Genetic Testing in Potential Living Kidney Donors of African Descent in Resource Limited Countries
O. Ekwenna¹, C. Okwuonu², I. Thoms³
¹University of Toledo College of Medicine, Toledo, OH, ²Nephrology and Medicine, Federal Medical Center Umuahia, Umuahia, Nigeria, ³Nephrology and Medicine, Mt. St John Medical Center, St. John, Antigua and Barbuda
*Purpose: To report our findings of APOL-1 testing in individuasl of African descent presenting as potential living kidney donors in resource limited countries; Antigua and…
2020 American Transplant Congress
Noninvasive Detection of Subclinical Rejection in Urine RNA
K. S. Keslar¹, D. Margeta¹, J. J. Friedewald², M. Abecassis², R. B. Mannon³, P. S. Heeger⁴, R. L. Fairchild¹
¹Cleveland Clinic, Cleveland, OH, ²Northwestern University, Chicago, IL, ³University of Alabama at Birmingham, Birmingham, AL, ⁴Icahn School of Medicine, New York, NY
*Purpose: Subclinical rejection may lead to long-term graft injury and can currently only be detected by repeated biopsies. A noninvasive method is needed to monitor…
2020 American Transplant Congress
A Monocyte-Derived MicroRNA Signature for Antibody-Mediated Rejection in Kidney Transplantation
C. Tinel¹, B. Lamarthée¹, F. Von Tokarski¹, M. Rabant², C. Legendre¹, W. Gwinner³, M. Naesens⁴, P. Marquet⁵, D. Anglicheau¹
¹Kidney Transplantation, Inserm U1151, Necker Hospital, Paris, France, ²Pathology, Necker Hospital, Paris, France, ³Nephrology, Hannover Medical School, Hannover, Germany, ⁴Nephrology and Renal Transplantation, University Hospitals Leuven, Leuven, Belgium, ⁵Nephrology, Dialysis and Transplantation, Inserm U850, Limoges University Hospital, Limoges, France
*Purpose: MicroRNAs (miRNAs) are small noncoding RNAs which regulate mRNA expression at the posttranscriptional level. We investigated blood miRNAs as non-invasive biomarkers in kidney transplantation…
2020 American Transplant Congress
The Effect of Human Thrombomodulin on Thrombotic Complications in Life Supporting Pig-to-Baboon Cardiac Xenotransplantation
C. E. Goerlich¹, A. Singh¹, D. Kaczorowski¹, M. Abdullah¹, B. Lewis¹, I. Tatarov¹, A. Hershfeld¹, T. Zhang¹, P. Odonkor¹, E. Strauss¹, B. Williams¹, D. Ayares², M. Mohiuddin¹
¹Surgery, University of Maryland School of Medicine, Baltimore, MD, ²Revivicor, Blacksburg, VA
*Purpose: Transplantation of organs from animals to humans (xenotransplantation) has been proposed to address the critical shortage of organs for transplantation. The use of genetically…
2020 American Transplant Congress
Femto Pulse Analysis of Exosome RNA from Plasma of Renal Transplant Patients Confirmed the Unique Benefits of Plasma Exosome for Messenger RNA (mRNA) Transcript Analysis
H. Zhang¹, K. Wong², S. C. Jordan³, D. Warzak², M. Toyoda¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, ²Agilent Technologies, Inc., Ankeny, IA, ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: Previously, we reported the feasibility of measuring the mRNA transcript levels of various genes in plasma exosomes of renal transplant patients for prediction of…
2019 American Transplant Congress
Results of Litmus (nct 02541916): The Liver Immune Tolerance Bio Marker Utilization Study
A. Chruscinski¹, V. Rojas-Luengas¹, S. Moshkelgosha¹, J. Luo², H. Yowanto², Z. Galvin¹, L. Lilly¹, R. Smith¹, E. Renner¹, J. Zhang¹, D. Grant¹, O. Adeyi¹, H. Atkins³, P. Juni⁴, N. Selzner¹, S. Juvet¹, G. Levy¹
¹Multi-Organ Transplantation, University Health Network, Toronto, ON, Canada, ²Sciex, Brea, CA, ³Ottawa Hospital Research Institute, Ottawa, ON, Canada, ⁴University of Toronto, Toronto, ON, Canada
*Purpose: Previously we reported a novel biomarker gene set for the identification of tolerance in murine models of rapamycin-induced cardiac tolerance and spontaneous hepatic tolerance.…