Articles tagged "Gene expression"

2020 American Transplant Congress
Gene Expression Signatures Associated with Antibody-Mediated Rejection (ABMR) in Renal Allograft Biopsies (Bx) Are Beneficial to Clarifying ABMR Activity in Ambivalent Cases
H. Zhang¹, C. C. Nast², N. Ammerman³, A. A. Vo³, I. Gallegos³, M. Arevalo³, S. C. Jordan³, M. Toyoda¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: “Thoroughly validated gene transcripts” in renal Bx was adopted as one of the criteria for ABMR diagnosis in the latest Banff classification. Here, we…
2020 American Transplant Congress
Antibiotic Pretreatment Alleviates Liver Transplant Damage by Modulating the Composition of Commensal Microbiota
K. J. Dery, S. Kageyama, H. Hirao, K. Kadono, H. Kojima, R. W. Busuttil, J. W. Kupiec-Weglinski
Surgery, Dumont-UCLA Transplant Center, UCLA, Los Angeles, CA
*Purpose: The human intestinal microbiome contains more than 100 trillion commensal microorganisms, exceeding the number of cells in the human body by 10-fold. Although recent…
2020 American Transplant Congress
Molecular Phenotypes of Liver Transplant Injury: The INTERLIVER Study
K. S. Madill-Thomsen¹, P. F. Halloran¹, &. the INTERLIVER Study Group²
¹Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ²., ., AB, Canada
*Purpose: Liver parenchymal injury phenotypes are difficult to evaluate by histology but could be amenable to molecular assessment. Having developed a Molecular Microscope® Diagnostic System…
2020 American Transplant Congress
A Comparison of Temporal Gene Expression Patterns in Kidney, Heart, Liver, and Lung Transplant Biopsies
P. Halloran¹, J. Reeve¹, .. the INTERLUNG Study Group², .. the INTERHEART Study Group², .. the INTERLIVER Study Group², &. the INTERCOMEX Study Group²
¹University of Alberta, Edmonton, AB, Canada, ²., ., AB, Canada
*Purpose: Gene expression patterns change with time post-transplant. The MMDx system makes it possible to do cross-organ comparisons using the same microarray platform and gene…
2020 American Transplant Congress
Routine Human Renal Allograft Biopsies Analyzed for mRNA Content with a Novel Nanostring mRNA Human Organ Transplant (HOT) Panel
I. Rosales¹, R. Smith¹, E. Acheampong¹, T. Kawai¹, T. Tsuji², K. Hotta³, R. Colvin¹
¹Massachusetts General Hospital, Boston, MA, ²Sapporo City General Hospital, Sapporo, Japan, ³Hokkaido University Hospital, Sapporo, Japan
*Purpose: In 2019 a Banff Working Group and Nanostring Technologies developed a panel of 770 mRNA probes for assessment of human organ allografts, based on…
2020 American Transplant Congress
Immune Checkpoint PD-L1 Pathway as an Effective Platform for Localized Immunomodulation to Attain Indefinite Allogeneic Islet Graft Survival
L. Batra¹, P. Shrestha¹, H. Zhao¹, K. B. Woodward¹, M. Tan¹, O. G. Nuno¹, A. Togay¹, M. M. Coronel², A. J. Garcia², H. Shirwan¹, E. S. Yolcu¹
¹Institute for Cellular Therapeutics and Department of Microbiology and Immunology, University of Louisville, Louisville, KY, ²Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA
*Purpose: Allogenic islet transplantation is an effective therapeutic approach for the treatment of T1D. However, chronic immunosuppression to control rejection is a major limitation. The…
2020 American Transplant Congress
Molecular Diagnosis of Rejection in Liver Transplant Biopsies: The INTERLIVER Study
K. S. Madill-Thomsen¹, P. F. Halloran², .. The INTERLIVER Study Group³
¹Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ²University of Alberta, Edmonton, AB, Canada, ³., ., AB, Canada
*Purpose: Diagnosing rejection in liver transplant biopsies is challenging given the limitations of histology e.g. low kappa values among pathologists. A Molecular Microscope® Diagnostic system…
2020 American Transplant Congress
Operational Tolerance in Kidney Transplant Recipients: Tomogram Transcriptomic Study
O. Viklicky¹, J. Klema², P. Mrazova¹, D. Abramowitz³, M. Abramowicz⁴, A. Massart³, P. Hruba¹
¹Institute for Clinical and Experimental Medicine, Prague, Czech Republic, ²Czech Technical University, Prague, Czech Republic, ³Antwerp University Hospital, Antwerp, Belgium, ⁴Université Libre de Bruxelles, Brussels, Belgium
*Purpose: TOMOGRAM is a European multicenter study, that aims to identify transcriptomic and genomic signatures of operational tolerance (OT) in a recently identified cohort of…
2020 American Transplant Congress
Lung Mucosal Biopsies Deteriorate with Time Post-Transplant: A Molecular Analysis
M. D. Parkes¹, P. Halloran¹, K. Halloran², &. the INTERLUNG Study Group³
¹Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ²University of Alberta, Edmonton, AB, Canada, ³., ., AB, Canada
*Purpose: Mucosal biopsies from the third airway bifurcation (3BMBs) assessed molecularly can be used to detect rejection (DOI: 10.1111/ajt.15685), and may also provide insights into…
2019 American Transplant Congress
Characterizing the Dominant Phenotype in Kidney Transplant Biopsies: Improving the Prediction of Risk by a Global View of Rejection, Injury, and Atrophy-Fibrosis
P. F. Halloran and The INTERCOMEX Investigators¹, J. Reeve²
¹University of Alberta, Edmonton, AB, Canada, ²Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada
*Purpose: Interpreting kidney transplant biopsies by histology or molecules involves assigning scores for separate pathologic categories: rejection, acute injury (molecules only), and chronic parenchymal loss…