Articles tagged "Gene expression"

2018 American Transplant Congress
Chronic Antibody Mediated Rejection in Renal Allografts Shows RNA Transcript Patterns That Evolve with Stage
A. Rosales,¹ B. Adam,² M. Mengel,² T. Oura,¹ M. Matsunami,¹ T. Kawai,¹ A. Cosimi,¹ R. Smith,¹ R. Colvin.¹
¹Massachusetts General Hospital, Boston, MA; ²University of Alberta, Edmonton, AB, Canada.
Purpose. Four stages of chronic antibody mediated rejection (CAMR) in renal allografts have been defined in humans and non-human primates (NHP), beginning with donor specific…
2018 American Transplant Congress
Molecular Classifiers Can Outperform the Flawed Histologic “Gold Standard” on Which They Are Trained
J. Reeve, P. Halloran.
University of Alberta, Edmonton, Canada.
The histologic diagnosis of rejection in organ transplants is known to be an imperfect gold standard. Therefore, it might be assumed that molecular classifiers built…
2018 American Transplant Congress
Defective Innate Immune Response to Opportunistic Pathogens in Cytomegalovirus-Infected Monocytes
P. Sen, A. R. Wilkie, R. Fernandes, R. J. Lane, S. E. Hickman, T. K. Means, E. R. Rosenberg, D. M. Coen, J. A. Fishman, J. El Khoury.
Transplant and Immunocompromised Group, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA; Department of Biological Chemistry and Pharmacology, Harvard Medical School, Boston, MA.
Human cytomegalovirus (CMV) is the most common infection encountered in transplantation and is an important cause of morbidity and mortality in both solid organ and…
2018 American Transplant Congress
Molecular Analysis of Acute Cellular Rejection in Clinical Face Transplants
T. Win, B. Dyring-Andersen, J. Teague, N. Murakami, A. Chandraker, B. Pomahac, L. Riella, R. Clark.
Brigham and Women's Hospital, Boston.
Although monitoring and immunosuppression of vascularized composite allografts (VCA) draw experience from solid organ transplantation, VCAs pose unique challenges. A deeper understanding of mechanisms underlying…
2018 American Transplant Congress
Whole-Transcriptome Sequencing to Identify Macaque Immune Gene Variants
A. Haj,¹ J. Karl,¹ R. Wiseman,¹ D. O'Connor.^1,2
¹Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI; ²Wisconsin National Primate Research Center, Madison, WI.
Macaque monkeys are critical in transplantation studies, yet macaque genomics lags significantly behind human genomics. While reliable genotyping methods have been developed for some macaque…
2018 American Transplant Congress
mRNA Transcript Profiling of 3 Genes in Renal Allograft Biopsies (Bx) Improves Accuracy of Antibody-Mediated Rejection (ABMR) Diagnosis in Transplant Recipients Desensitized with IVIG+Rituximab+Immunoglobulin G-Degrading Enzyme of Streptococcus Pyogenes (IdeS)
H. Zhang,¹ C. Nast,² J. Doldron,² M. Cheung,² A. Vo,³ S. Jordan,³ M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA; ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA; ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA.
Background: Multiple molecular signatures specific to ABMR based on gene expression in renal allograft Bx of transplant recipients were reported. Here we assessed mRNA transcript…
2018 American Transplant Congress
Predictors of Graft Survival at the Time of a Kidney Transplant Indication Biopsy
G. Einecke,¹ J. Reeve,^2,3 P. Halloran.^2,4
¹Hannover Medical School, Hannover, Germany; ²Alberta Transplant Applied Genomics Centre, Edmonton, Canada; ³Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada; ⁴Medicine, University of Alberta, Edmonton, Canada.
We predicted kidney transplant graft survival after indication biopsy in 884 patients. Analysis was performed using random forests, a multivariate machine learning method to assess…
2018 American Transplant Congress
Overexpression of BAX, NOL3, and XIAP Apoptotic Genes Participates in Calcineurin Inhibitors Nephrotoxicity: New Insights into Chronic Allograft Dysfunction
L. Garcia-Covarrubias,¹ G. Queipo,² M. Fonseca,² D. Alcantara,¹ P. Prieto,¹ H. Diliz,¹ A. Garcia,¹ H. Hinojosa,¹ A. Cicero,¹ G. Melendez,³ M. Alamilla,³ R. Lascurain,⁴ V. Soto.⁵
¹Transplantation, Hospital General de Mexico, Mexico City, Mexico; ²Genetics, Hospital General de Mexico, Mexico City, Mexico; ³Research, Hospital General de Mexico, Mexico City, Mexico; ⁴Faculty of Medicine, UNAM, Mexico City, Mexico; ⁵Pathology, Hospital General de Mexico, Mexico City, Mexico.
Introduction: Calcineurin inhibitors toxicity (CNIT) is a factor that limit the outcome of renal transplantation graft. The hypoxia causes histological data as nodular hyalinosis, hyalinosis,…
2018 American Transplant Congress
Hypothermic Machine Perfusion Protects DCD Liver Graft through Metabolism of Xenobiotics by Cytochrome P450 Pathway
Q. Ye,^1,2 W. Liang,¹ Z. Zhong,¹ S. Ye,¹ B. Tang,¹ Q. Hu,¹ J. Liang,¹ Y. Wang.¹
¹Transplant Center of Wuhan University, Zhongnan Hospital of Wuhan University, Wuhan, China; ²The 3rd Xiangya Hospital of Central South Unversity, Changsha, China.
Background: Donation after cardiac death (DCD) is the mainly resource to relieve organ-transplantation shortage. Our previous research have confirmed that the function of DCD liver…
2018 American Transplant Congress
Molecular Assessment of Pre-Implantation Donor Biopsies: Effects of Prolonged Cold Ischemia Time and Development of Delayed Graft Function
E. Akalin,¹ Y. Bao,¹ V. Bontha,² M. e Silva,³ M. Ajaimy,¹ P. [Oacute] Broin,³ V. Mas.²
¹Montefiore Medical Center, Bronx; ²University of Virginia, Charlottesville; ³National University of Ireland Galway, Galway, Ireland.
Background: Deceased-donor (DD) kidneys are at higher risk for ischemia/reperfusion injury leading to increased inflammatory mediators and innate immune response. We aimed to investigate the…