Date: Saturday, May 30, 2020
Session Time: 3:15pm-4:00pm
Presentation Time: 3:30pm-4:00pm
*Purpose: The outcome of kidney transplantation is highly affected by donor category. Little is known about molecular processes which may underline this phenomenon.
*Methods: Using Molecular Microscope Diagnostic System (MMDx) we evaluated molecular scores in early indication biopsies (median 13 POD) diagnosed as Banff borderline category and subsequent 3M follow-up biopsies in three cohorts: expanded criteria donor (ECD, n=22), standard criteria donor (SCD, n=12) and living donor (LD, n=12) cohorts.
*Results: Compared to LD, both ECD and SCD kidney grafts exhibited higher acute kidney injury (AKI) score in early indication biopsies (p=0.012 and p=0.022, respectively). At 3-month follow-up biopsies the AKI was similar between ECD and SCD kidney grafts and decreased in both ECD (p=0.019) and SCD (p=0.015) in comparison to early biopsies. Similarly, higher atrophy-fibrosis scores in indication biopsies were found in both ECD (p=0.012) and SCD (p=0.004) as compared to LD kidney grafts. At 3-month follow-up biopsies the atrophy-fibrosis score increased in all donor categories (ECD, p=0.086; SCD, p=0.054; LD, p=0.027), in comparison with early biopsies while the differences among categories persisted (Fig 1). Donor categories had no effect neither on inflammation score nor rejection scores in both indication and follow-up biopsies.
Figure 1. MMDx scores A. Acute kidney injury score B. Atrophy-fibrosis score C. Inflammation score in early indication biopsy and follow-up 3-months biopsy in particular donor subcategories.
*Conclusions: MMDx acute kidney injury score reflects donor quality in early biopsies and resolved within 3 months. Interestingly, atrophy-fibrosis score deteriorated in all donor categories, living donor included, which pointed out to non-reversible changes induced by peritransplant injuries regardless the cold ischemia time.
To cite this abstract in AMA style:Hruba P, Madill-Thomsen KS, Mackova M, Maluskova J, Honsova E, Slatinska J, Halloran PF, Viklicky O. Evolution of Molecular MMDx Scores after Kidney Transplantationin Different Donor Categories [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/evolution-of-molecular-mmdx-scores-after-kidney-transplantationin-different-donor-categories/. Accessed October 28, 2020.
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