Articles tagged "B cells"

2019 American Transplant Congress
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Lymphocyte Biology and Transplant Immunity
L. Qiu¹, J. Wang², R. Fernandez³, X. Yeap¹, S. Han¹, F. Zheng¹, C. Lin², Z. J. Zhang¹
¹Comprehensive Transplant Center, Department of Surgery, Northwestern University, Chicago, IL, ²Northwestern University, Chicago, IL, ³Department of Surgery, Northwestern University, Chicago, IL
*Purpose: Spleen-derived lymphocytes and myeloid cell populations are critical to the development of organ transplant ischemia-reperfusion injury (IRI) and allograft rejection. In this study, we…
2019 American Transplant Congress
Costimulation Blockade Modulates Humoral Responses in Highly Sensitized Transplant Recipients
J. Kwun, B. Ezekian, P. Schroder, M. Manook, J. Yoon, M. Mulvihill, K. Freischlag, J. Yi, S. Knechtle
Surgery, Duke Transplant Center, Durham, NC
*Purpose: Broad human leukocyte antigen sensitization is a significant barrier to successful solid organ transplantation. Sensitized patients have poor access to donor organs, high wait…
2019 American Transplant Congress
IL-1β and IL-6 Facilitate T Cell Reconstitution in Heart Allograft Recipients Treated with Anti-Thymocyte Globulin
S. Hasgur, R. Fan, A. Valujskikh
Inflammation and Immunity, Cleveland Clinic, Cleveland, OH
*Purpose: Antibody-mediated lymphocyte depletion is used in solid organ and stem cell transplantation. However, T cell reconstitution after lymphoablation may lead to acute rejection and…
2019 American Transplant Congress
IL-10+ Plasma Cells Are Not Required for Breg Activity and Are Counterbalanced by Novel Pro-Inflammatory Plasma Cells
Q. Ding, Y. Zhou, Z. Song, L. Zeng, D. Rothstein
University of Pittsburgh, Pittsburgh, PA
*Purpose: Classically, B cells differentiate into Ab-secreting plasma cells (PCs; B220lowCD138hiBlimp1+Ig+) providing humoral immunity. B cells can also inhibit or promote immune responses through the…
2019 American Transplant Congress
Regulation of Host microRNA Expression by Natural Variants of Epstein Barr Virus (EBV) Latent Membrane Protein 1 (LMP1)
O. Hatton¹, A. Harris-Arnold², A. Hui², E. Maloney², S. M. Krams², O. M. Martinez²
¹Molecular Biology, Colorado College, Colorado Springs, CO, ²Program in Immunology and Department of Surgery, Stanford University, Stanford, CA
*Purpose: Malignancies attributable to EBV, such as post-transplant lymphoproliferative disorder (PTLD), represent 1.8% of global cancer deaths. PTLD occurs in 1-20% of transplant recipients and…
2019 American Transplant Congress
Interleukin-27 is Required for Donor Specific Antibody Production in Response to Heart Allograft Transplantation
M. Nicosia, S. Miyairi, R. Fan, A. Beavers, V. Gorbacheva, A. Valujskikh
Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH
*Purpose: Interleukin-27 (IL-27), a member of IL-12 cytokine family, is involved in both pro- and anti-inflammatory immune responses. Previously we reported that B cell derived…
2019 American Transplant Congress
EBV⁺ PTLD Cell Lines Secrete Exosomes Containing microRNAs That Alter Immune Responses
S. Syed, E. Maloney, A. Hui, O. Martinez, S. Krams
Stanford Univ School of Med, Stanford, CA
*Purpose: Exosomes are nanometric (50-150nm) membrane vesicles that are released, into blood and other body fluids, by most cell types. Exosomes, which are generated from…
2019 American Transplant Congress
Circulating T Follicular Helper Proliferation And Memory B Cell Activation Correlate With Donor Specific Allo-antibody Occurrence And Antibody-mediated Rejection After Kidney Transplantation
K. Louis¹, C. Macedo¹, M. Marrari¹, B. Ramaswami¹, M. Lucas¹, B. Elinoff¹, M. Yamada¹, X. Gu¹, G. Chalasani¹, A. Zeevi¹, P. Randhawa¹, C. Lefaucheur², D. Metes¹
¹Surgery, University of Pittsburgh, Pittsburgh, PA, ²Inserm Unité mixte de recherche 1160, Paris, France
*Purpose: Crosstalk between T follicular helper (Tfh) cells and B cells is necessary for class-switched antibody generation. The contribution of memory Tfh and B cells…
2019 American Transplant Congress
B Cell Immunophenotypes in Transplantation
M. Cascalho¹, M. de Mattos-Barbosa², E. A. Farkash³, J. L. Platt²
¹University of Michigan, Ann Arbor, MI, ²Surgery, University of Michigan, Ann Arbor, MI, ³Pathology, University of Michigan, Ann Arbor, MI
*Purpose: B cells and B cell responses have been associated with favorable outcomes of transplantation in some setting and unfavorable outcomes in others. For example,…
2019 American Transplant Congress
Novel In Vitro Germinal Center Culture System Demonstrates More Aggressive B Cell to Plasma Blast Differentiation in Allosensitized Nonhuman Primates
P. Schroder, J. Yoon, R. Schmitz, Z. Fitch, J. Kwun, S. J. Knechtle
Department of Surgery, Duke University Medical Center, Durham, NC
*Purpose: The allogeneic humoral immune response is largely composed of T cell dependent antibody responses produced after B and T cells interact within the germinal…