Articles tagged "Alloantibodies"

2021 American Transplant Congress
Engineered Human Glomerular Endothelial Cells to Identify Non-hla Antibodies and Decipher Their Pathogenicity After Kidney Transplantation
B. Lamarthée¹, C. Burger¹, C. Leclaire¹, L. Morin¹, F. Terzi¹, C. Tinel¹, D. Anglicheau²
¹Inserm U1151, Necker-Enfants Malades Institute, PARIS, France, ²Necker Hospital, AP-HP, Department of Nephrology and Kidney Transplantation, PARIS, France
*Purpose: In kidney transplantation recipients (KTRs), donor-specific antibodies directed against Human Leucocyte Antigens (HLA-DSA) are thought to drive antibody-mediated rejection (AMR) and poor transplant outcome.…
2021 American Transplant Congress
Belatacept and Carfilzomib-based Treatment of Active Antibody-mediated Rejection in a Sensitized Nonhuman Primate Model
J. Kwun¹, R. Schmitz¹, M. Manook¹, Z. Fitch¹, D. Olaso¹, A. Choi¹, J. Yoon¹, Y. Bae¹, J. Lambris², S. Knechtle¹
¹Surgery, Duke University, Durham, NC, ²Pathology & Laboratory Medicine, University of Pennsylvania, Philadelphia, PA
*Purpose: One third of sensitized, HLA-incompatible transplant recipients experience antibody-mediated rejection (AMR) with limited effective treatment options. We tested a novel treatment strategy for AMR…
2021 American Transplant Congress
A Molecular Classifier Identifies Antibody-Mediated Rejection-Associated Changes in Lung Transplant Biopsies
K. S. Madill-Thomsen¹, K. M. Halloran², M. Parkes¹, P. F. Halloran¹, &. the INTERLUNG Study Group³
¹Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ²University of Alberta, Edmonton, AB, Canada, ³., ., AB, Canada
*Purpose: Identifying lung transplant antibody-mediated rejection (ABMR) has been controversial. We compared gene expression in donor-specific antibody (DSA) positive vs. DSA negative lung transbronchial biopsies…
2021 American Transplant Congress
Impact of Allosensitization on Xenotransplantation
D. Olaso¹, M. Manook¹, J. Yoon¹, Y. Bae¹, A. Barbas¹, A. Adams², S. Knechtle¹, J. Kwun¹
¹Department of Surgery, Duke Transplantation Center, Durham, NC, ²Department of Surgery, University of Minnesota, Minneapolis, MN
*Purpose: Sensitization to human leukocyte antigens (HLA) due to prior exposure to foreign antigens through events such as blood transfusions, previous kidney transplant, or pregnancy…
2021 American Transplant Congress
Perioperative Blood Transfusion is Associated with the Development of New HLA Antibodies After Adult Lung Transplantation
A. Hicks¹, A. Stoker², M. Cooter², A. Ali³, J. Klapper⁴, J. Poisson⁵, D. Chen⁵, J. Haney⁴, K. Ghadimi², M. Hartwig⁴, I. Welsby², B. Bottiger²
¹Anesthesiology, Duke University Hospital, South Portland, ME, ²Anesthesiology, Duke University Hospital, Durham, NC, ³Pulmonology, Allergy and Critical Care Medicine, Duke University Hospital, Durham, NC, ⁴Cardiothoracic Surgery, Duke University Hospital, Durham, NC, ⁵Pathology, Duke University Hospital, Durham, NC
*Purpose: Lung transplant (LT) recipients who develop new antibodies to human leukocyte antigen (HLA) have associated with chronic lung allograft dysfunction and worsened outcomes. We…
2021 American Transplant Congress
Belatacept and Rapamycin Maintenance Immunosuppression in a Sensitized Nonhuman Primate Kidney Allotransplantation Model
R. Schmitz¹, Z. W. Fitch¹, M. Manook¹, J. Yoon¹, A. B. Farris², J. Kwun¹, S. J. Knechtle¹
¹Department of Surgery, Duke University Medical Center, Durham, NC, ²Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA
*Purpose: Desensitization with costimulation blockade and proteasome inhibition prior to kidney transplantation prevents early antibody-mediated rejection (AMR) and prolongs graft survival in highly sensitized nonhuman…
2021 American Transplant Congress
Identification of Distinct Blood Monocyte Phenotypes from Transplant Kidney Recipients Undergoing Cellular (TCMR) and Mixed Antibody Mediated Rejection (ABMR)
C. Macedo, E. Bailly, K. Louis, M. Lucas, A. Zeevi, P. Randhawa, D. Metes
Starzl Transplantation Institute, Pittsburgh, PA
*Purpose: Monocytes play an important role in immune defense by initiating adaptive immunity. They are heterogeneous, plastic, and present different subsets: classical (CD14++CD16-), intermediate (CD14+CD16+),…
2021 American Transplant Congress
Autoantibodies Against Ro/SS-A, CENP-B, and La/SS-B are Increased in Patients with Kidney Allograft Antibody-mediated Rejection
S. Clotet-Freixas¹, M. Kotlyar², C. M. McEvoy¹, C. Pastrello², S. Rodríguez-Ramírez¹, S. Farkona¹, H. Cardinal³, M. Dieudé³, M. Hébert³, Y. Li¹, O. Famure¹, P. Chen¹, S. Kim¹, E. Chan⁴, I. Jurisica², R. John¹, A. Chruscinski¹, A. Konvalinka¹
¹Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada, ²Krembil Research Institute, University Health Network, Toronto, ON, Canada, ³Centre de Recherche du Centre Hospitalier de l’Université de Montréal, Montreal, QC, Canada, ⁴Department of Medicine, Division of Nephrology, University Health Network, Toronto, ON, Canada
*Purpose: Antibody-mediated rejection (AMR) causes >50% of late kidney graft losses. Although donor-specific antibodies (DSA) against HLA cause AMR, antibodies against non-HLA antigens are also…
2021 American Transplant Congress
DSA is Associated with Molecular Changes in Many Hearts with Minor Abnormalities but Not Diagnosed as Antibody-Mediated Rejection
P. Halloran¹, K. S. Madill-Thomsen¹, &. the INTERHEART Study Group²
¹Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ²., ., AB, Canada
*Purpose: We previously developed a microarray-based diagnostic system (MMDx) for heart transplant endomyocardial biopsies, measuring gene expression and interpreting antibody-mediated and T cell-mediated rejection (ABMR,…
2021 American Transplant Congress
Quantifying Hidden Sensitization: HLA-reactive Memory B Cells in the Spleens of Sensitized Women
J. T. Killian¹, J. A. Houp¹, A. Nellore², R. G. King³, P. M. Porrett¹, F. E. Lund³
¹Department of Surgery, University of Alabama at Birmingham School of Medicine, Birmingham, AL, ²Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, AL, ³Department of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL
*Purpose: Measurement of circulating anti-HLA antibody (anti-HLA-Ab) in transplant candidates and recipients is required for successful organ transplantation. However, serum Ab measurement alone likely underestimates…