T Cell Transfer With iNKT Cell Activation Facilitate Engraftment of Bone Marrow Cells Without GvHD
1Department of Urology, Tokyo Women's Medical University, Tokyo, Japan
2Laboratory of Vaccine Design, RIKEN IMS-RCAI, Yokohama, Japan.
Meeting: 2015 American Transplant Congress
Abstract number: B62
Keywords: Graft-versus-host-disease, Mixed chimerism, T cells, Tolerance
Session Information
Session Name: Poster Session B: Cell Transplantation and Cell Therapies
Session Type: Poster Session
Date: Sunday, May 3, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Contamination of allogenic T cells is considered to be a risk factor of graft versus host disease (GvHD) in bone marrow transplantation (BMT), whereas it is also known that allogenic T cells act as essential partner cells for allogeneic BM engraftment. We previously showed that a single administration of liposomal formulation of α-glactosylceramide (lipo-αGC), which can emphasize regulatory aspect of invariant natural killer T cells, could prevent acute GvHD in lethally irradiated BMT model even when allogenic T cells were co-transferred. Therefore, we investigated whether allogenic T cell transfer can promote donor BM engraftment without GvHD when administered with Lipo-αGC in non-myeloablative fully allogeneic BMT model. 3Gy irradiated BALB/c mice (H2d) were grafted 5 x 10^6 – 20 x 10^6 C57BL/6 (H2b) bone marrow cells (BMCs) with or without transfer of 2 x 10^5 T cells obtained from the same donor with administration of lipo-αGC. Anti-CD154 mAb (0.5mg) was also administered to support BM-engraftment in the current study. All mice without T cell transfer showed failure of donor-cell engraftment when grafted less than 10 x 10^6 donor BMCs, whereas those with T cell transfer showed successful engraftment even in 5 x 10^6 BMCs (Fig1). Importantly, mice engrafted donor T cells did not show any remarks of GvHD. Engrafted T cells seemed to be newly developed ones from T cell precursors contained in transplanted BMCs through the thymus rather than injected T cells themselves, because significant decrease of host-reactive Vβ5+T cells were shown in donor-derived T cells of our chimera mice (Fig.2). Since the thymus of mice transplanted with lipo-αGC showed earlier recovery of the number of thymocytes compared to those without, we thought that lipo-αGC controlled GvHD through thymic regeneration. Thus, we concluded that allogenic T cells can support donor BM engraftment without GvHD when administered with lipo-αGC, offering a novel and potentially safe approach for attaining stable hematopoietic chimerism.
To cite this abstract in AMA style:
Ishii R, Hirai T, Miyairi S, Ikemiyagi M, Omoto K, Ishii Y, Tanabe K. T Cell Transfer With iNKT Cell Activation Facilitate Engraftment of Bone Marrow Cells Without GvHD [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/t-cell-transfer-with-inkt-cell-activation-facilitate-engraftment-of-bone-marrow-cells-without-gvhd/. Accessed May 31, 2025.« Back to 2015 American Transplant Congress