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T Cell Transfer With iNKT Cell Activation Facilitate Engraftment of Bone Marrow Cells Without GvHD

R. Ishii,1 T. Hirai,1 S. Miyairi,1 M. Ikemiyagi,1 K. Omoto,1 Y. Ishii,2 K. Tanabe.1

1Department of Urology, Tokyo Women's Medical University, Tokyo, Japan
2Laboratory of Vaccine Design, RIKEN IMS-RCAI, Yokohama, Japan.

Meeting: 2015 American Transplant Congress

Abstract number: B62

Keywords: Graft-versus-host-disease, Mixed chimerism, T cells, Tolerance

Session Information

Session Name: Poster Session B: Cell Transplantation and Cell Therapies

Session Type: Poster Session

Date: Sunday, May 3, 2015

Session Time: 5:30pm-6:30pm

 Presentation Time: 5:30pm-6:30pm

Location: Exhibit Hall E

Contamination of allogenic T cells is considered to be a risk factor of graft versus host disease (GvHD) in bone marrow transplantation (BMT), whereas it is also known that allogenic T cells act as essential partner cells for allogeneic BM engraftment. We previously showed that a single administration of liposomal formulation of α-glactosylceramide (lipo-αGC), which can emphasize regulatory aspect of invariant natural killer T cells, could prevent acute GvHD in lethally irradiated BMT model even when allogenic T cells were co-transferred. Therefore, we investigated whether allogenic T cell transfer can promote donor BM engraftment without GvHD when administered with Lipo-αGC in non-myeloablative fully allogeneic BMT model. 3Gy irradiated BALB/c mice (H2d) were grafted 5 x 10^6 – 20 x 10^6 C57BL/6 (H2b) bone marrow cells (BMCs) with or without transfer of 2 x 10^5 T cells obtained from the same donor with administration of lipo-αGC. Anti-CD154 mAb (0.5mg) was also administered to support BM-engraftment in the current study. All mice without T cell transfer showed failure of donor-cell engraftment when grafted less than 10 x 10^6 donor BMCs, whereas those with T cell transfer showed successful engraftment even in 5 x 10^6 BMCs (Fig1). Importantly, mice engrafted donor T cells did not show any remarks of GvHD. Engrafted T cells seemed to be newly developed ones from T cell precursors contained in transplanted BMCs through the thymus rather than injected T cells themselves, because significant decrease of host-reactive Vβ5+T cells were shown in donor-derived T cells of our chimera mice (Fig.2). Since the thymus of mice transplanted with lipo-αGC showed earlier recovery of the number of thymocytes compared to those without, we thought that lipo-αGC controlled GvHD through thymic regeneration. Thus, we concluded that allogenic T cells can support donor BM engraftment without GvHD when administered with lipo-αGC, offering a novel and potentially safe approach for attaining stable hematopoietic chimerism.

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To cite this abstract in AMA style:

Ishii R, Hirai T, Miyairi S, Ikemiyagi M, Omoto K, Ishii Y, Tanabe K. T Cell Transfer With iNKT Cell Activation Facilitate Engraftment of Bone Marrow Cells Without GvHD [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/t-cell-transfer-with-inkt-cell-activation-facilitate-engraftment-of-bone-marrow-cells-without-gvhd/. Accessed May 9, 2025.

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