2018 American Transplant Congress
Immunity and Tolerance after Bilateral Orthotopic Lung Transplant (BOLT) in Tandem with a CD3+/CD19+ Depleted Vertebral Bone Marrow Transplant (BOLT+BMT) from 1 of 8 HLA-Matched Cadaveric Donors
Primary immunodeficiency patients may develop pulmonary complications and most are ineligible for either lung transplant or BMT due to futility. We report our first 2…2018 American Transplant Congress
Absolute Quantification of Plasma Graft-Derived Cell-Free DNA for Diagnosis of Kidney Transplant Rejection
Aim: To study the performance of droplet digital PCR absolute measurement of graft-derived cell-free DNA (gdcfDNA) for the diagnosis of kidney allograft rejection.Methods: Homozygous deletion…2018 American Transplant Congress
A Novel Conditioning Approach to Establish Chimerism Using Manipulation of JAK-STAT Pathways
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY.
Hematopoietic chimerism establishes donor-specific tolerance to solid organ allografts. Targeting recipient immune cells (T, NK cells and macrophages) can effectively reduce the dose of total…2018 American Transplant Congress
Tomotherapy and Donor Hematopoietic Cells for Tolerance to Kidney Transplants in Rhesus Macaques
The goal of this project was to develop a tolerance induction protocol for MHC 1-haplotype mismatched kidney transplants (txs) in a rhesus macaque model. The…2018 American Transplant Congress
Immunomodulation of Alloantibody Responses
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.
We have shown that i.v. exposure to MHC-mismatched donor PBMC using a novel, mild conditioning protocol results in B-cell tolerance with transient T-cell unresponsiveness. Here…2018 American Transplant Congress
Predicting Outcomes of Rat Vascularized Composite Allotransplants through Quantitative Measurement of Chimerism with PCR-Amplified Short Tandem Repeat
Background: Chimerism, defined as coexistence of donor and recipient cells, has been associated with induction and maintenance of tolerance to allotransplants. Although most studies have…2018 American Transplant Congress
CD8-Expressing Transitional B Cells Dominate B Cell Reconstitution in Transplanted Monkeys Receiving Mixed Chimerism Conditioning
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.
IntroductionTolerance of heart or lung allografts have been achieved for the first time in nonhuman primates (NHPs) using a mixed chimerism conditioning regimen. However, the…2018 American Transplant Congress
Enhancement of Intrinsic Apoptosis via Bcl-2 Inhibition Promotes Induction of Robust Mixed Chimerism and Renal Allograft Tolerance without Myelosuppression in Nonhuman Primates
Background: Successful induction of mixed chimerism (MC) and renal allograft tolerance has been achieved in both nonhuman primate (NHP) and man following conditioning that included…2018 American Transplant Congress
Robust Hematopoietic Mixed Chimerism and Donor-Specific Skin Allograft Tolerance after Non-Genotoxic Anti-CD117 Immunotoxin Conditioning and Donor Bone Marrow Allotransplantation
Establishing hematopoietic chimerism enables donor-specific allograft tolerance; however, chimerism protocols typically require either genotoxic conditioning or mega-doses of donor bone marrow cells (BMC), impeding clinical…2018 American Transplant Congress
PD-L1 May Serve as a Biomarker for Chimerism-Induced Tolerance in Kidney Transplant Recipients
1University of Louisville, Louisville, KY; 2Northwestern Memorial Hospital, Chicago, IL.
Durable chimerism is currently the most reliable biomarker of donor specific tolerance. Identifying and validating other predictive biomarkers is therefore a high priority. Since 2009…
American Transplant Congress abstracts