Simultaneous Persistence and Development of Donor and Recipient Tissue Resident Memory T Cells in Lung Transplantation
1Medicine, Columbia University Medical Center, New York, NY
2Pediatrics, Columbia University Medical Center, New York, NY
3Pathology, Columbia University Medical Center, New York, NY
4Surgery, Columbia University Medical Center, New York, NY
5Microbiology & Immunology, Columbia University Medical Center, New York, NY.
Meeting: 2018 American Transplant Congress
Abstract number: C273
Keywords: Immunogenicity, Lung transplantation, Lymphocytes, T cells
Session Information
Session Name: Poster Session C: Lymphocyte Biology: Signaling, Co-Stimulation, Regulation
Session Type: Poster Session
Date: Monday, June 4, 2018
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall 4EF
Introduction: The predominant T cell population in the lung comprises non-circulating tissue-resident memory T cells (TRM); however, the fate of these cells in transplanted lung allograft is not known. Here, we investigated the phenotype and function of persisting donor-derived T cells along with the development of recipient-derived T cells in bronchoalveolar lavage (BAL) samples of lung transplant recipients.
Methods: Donor and recipient lymphocyte populations (determined by staining for HLA discrepancies) were analyzed phenotypically and functionally from BAL samples collected prospectively for 17 lung transplant recipients. Profiles were compared to lung and BAL obtained from organ donors as healthy controls.
Results: Donor derived CD4 and CD8 T cells from BAL exhibited predominant TRM phenotypes expressing CD69 and CD103, with increased CD69 (p < 0.01) and CD103 (p<0.01) expression between 2-12 months post-transplant, exceeding that of healthy normal lung (
: TEM = effector memory T cells, gray bar = range from healthy donors). There was evidence for gradual accumulation of TRM phenotypes by recipient-derived T cells over time, reaching similar profile as TRM in healthy lungs by 6 months post-transplant (Fig 1). Functionally, donor TRM exhibited higher capacity for production of IFN-γ (0.005) compared to recipient lymphocytes, and exhibited reduced CD28 expression over time in compared to recipients (p = 0.03).
Conclusions: Recipient derived lymphocytes within the lung allograft achieve tissue residency homeostasis within 6 months following lung transplantation, but appear to have reduced functional capacity when compared to long-lived donor derived TRMs.
CITATION INFORMATION: Snyder M., Connors T., Marboe C., Benvenuto L., Shah L., Robbins H., Hook J., D'Ovidio F., Bacchetta M., Sonnett J., Arcasoy S., Farber D. Simultaneous Persistence and Development of Donor and Recipient Tissue Resident Memory T Cells in Lung Transplantation Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Snyder M, Connors T, Marboe C, Benvenuto L, Shah L, Robbins H, Hook J, D'Ovidio F, Bacchetta M, Sonnett J, Arcasoy S, Farber D. Simultaneous Persistence and Development of Donor and Recipient Tissue Resident Memory T Cells in Lung Transplantation [abstract]. https://atcmeetingabstracts.com/abstract/simultaneous-persistence-and-development-of-donor-and-recipient-tissue-resident-memory-t-cells-in-lung-transplantation/. Accessed November 21, 2024.« Back to 2018 American Transplant Congress