ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Preservation of Pancreatic Islets with Stem Cell Derived CD34+ Vascular Endothelial Progenitor Cells

C. Ward1, L. Vo2, E. De Klerk2, G. Faleo2, Y. Kelly1, J. Bluestone2, M. Hebrok2, P. Stock1, Q. Tang1

1Surgery, UCSF, San Francisco, CA, 2Diabetes Center, UCSF, San Francisco, CA

Meeting: 2019 American Transplant Congress

Abstract number: 367

Keywords: Bioengineering, Endothelial cells, Islets, Stem cells

Session Information

Date: Monday, June 3, 2019

Session Name: Concurrent Session: Islet and Cell Transplantation

Session Time: 4:30pm-6:00pm

 Presentation Time: 4:54pm-5:06pm

Location: Room 309

Related Abstracts
  • Parathyroid CD34+ Cells Induce Neovascularization of Donor and Recipient Leading to Chimeric Vessel Formation and Improved Engraftment of Co-Transplanted Pancreatic Islets
  • The Role of Natural Killer Cells in Recognition and Killing of Stem Cells and Stem Cell-Derived Hepatoblasts.

*Purpose: Islet transplantation can cure Type 1 diabetes; however, multiple donors are needed due to extensive perioperative loss of islets when removed from their native blood supply. We recently discovered that CD34+45- vascular endothelial cells from parathyroid glands (PTG), another richly vascular endocrine organ, have an extraordinary ability to promote beta cell engraftment and function. This study aimed to generate a human embryonic stem-cell (hESC) derived source of CD34+45- vascular endothelial cells that could equitably protect islet grafts.

*Methods: hESC-derived β-cells (eBCs) and CD34+45- (scCD34+) cells were differentiated from the same stem cell line and were co-transplanted in the subcutaneous (SQ) and intramuscular (IM) sites of immunodeficient mice. scCD34+ cells were FACS purified.

*Results: Using the same hESC line that we have used to produce eBCs, we have succeeded in generating CD34+CD45- cells after 9 days of in-vitro differentiation. These scCD34+ cells lack the hematopoietic marker CD45 and contain the endothelial precursor marker CD146+ (Fig A). Moreover, these cells efficiently formed tubular structures in 4 hours in an in vitro angiogenesis assay, suggesting that these cells are vascular endothelial progenitor cells (Fig B). Transplantation of scCD34+ cells in an in-vivo angiogenesis model, using mouse skin flaps, resulted in increased vascular density and number of vessel junctions compared to sham and hematopoietic CD34+45+ cells differentiated simultaneously. Co-transplantation of scCD34+ with eBCs in SQ and IM sites resulted in significant improvement of graft mass preservation when compared with eBC transplanted alone. Moreover, we developed a novel co-clustering method to combine scCD34+ with eBCs prior to transplantation, creating vascularized beta cells, which showed further engraftment protection in the SQ and IM via luciferase imaging when compared to control.

*Conclusions: While the βετα-cell-protective activity of PTG CD34+ cells is remarkable, a renewable source of CD34+ cells that possess the same activities of PTG CD34+ cells is highly desired to enable wide application of βετα-cell replacement therapy. This study show that vascular progenitor cells can be generated from stem cells and used to support the engraftment of stem cell derived beta cells.

 border=

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Ward C, Vo L, Klerk EDe, Faleo G, Kelly Y, Bluestone J, Hebrok M, Stock P, Tang Q. Preservation of Pancreatic Islets with Stem Cell Derived CD34+ Vascular Endothelial Progenitor Cells [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/preservation-of-pancreatic-islets-with-stem-cell-derived-cd34-vascular-endothelial-progenitor-cells/. Accessed February 28, 2021.

« Back to 2019 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.