Articles tagged "Endothelial cells"

2020 American Transplant Congress
The Role of Galectin-1 and Basement Membrane Remodeling in Kidney Antibody-Mediated Rejection
S. Clotet-Freixas¹, C. McEvoy¹, I. Batruch², M. Kotlyar³, C. Pastrello³, J. Van¹, M. Arambewela¹, A. Boshart¹, S. Farkona¹, A. Bozovic⁴, V. Kulasingam⁴, P. Chen¹, I. Jurisica³, A. Chruscinski¹, R. John⁴, A. Konvalinka⁵
¹Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada, ²Department of Laboratory Medicine and Pathobiology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada, ³Krembil Research Institute, University Health Network, Toronto, ON, Canada, ⁴Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, ⁵Department of Medicine, Division of Nephrology, University Health Network, Toronto, ON, Canada
*Purpose: Antibody-mediated rejection (AMR), the leading cause of kidney graft loss, is caused by anti-HLA and non-HLA antibodies against proteins in two main kidney compartments:…
2020 American Transplant Congress
Treg Protection of the Allograft Depends on Lymphotoxin Beta Receptor Expression in Lymphatic Endothelial Cells
V. Saxena¹, W. Piao¹, L. Li¹, Y. Xiong¹, C. Palusckievicz¹, T. Simon¹, M. Shirkey¹, R. Abdi², J. S. Bromberg¹
¹University of Maryland, Baltimore, Baltimore, MD, ²Harvard U, Boston, MA
*Purpose: Regulatory T cell (Treg) migration to afferent lymphatics and lymph nodes (LN), and suppressive function, require Treg lymphotoxin alpha (LTα) to stimulate LT beta-receptor…
2020 American Transplant Congress
Single Cell Analysis Reveals Endothelial Activation of Interleukin-6 Mediated Signaling Pathway in Microvascular Inflammation with and without c4d
A. S. Naik¹, R. Menon¹, E. Farkash¹, E. Otto¹, C. C. Berthier¹, S. Norman¹, M. Samaniego², M. Kretzler¹
¹University of Michigan, Ann Arbor, MI, ²Henry Ford Hospital, Detroit, MI
*Purpose: Microvascular inflammation (MVI) is a feature of antibody mediated rejection. MVI can be seen with or without activation of other effectors of antibody injury…
2020 American Transplant Congress
Cyclophilin-D Inhibition Mitigates Cardiac Allograft Ischemia-Reperfusion Injury and Rejection
A. Qamar¹, P. McLeod², J. Jiang², X. Huang², A. M. Jevnikar², Z. Zhang²
¹Pathology & Laboratory Medicine, University of Western Ontario, London, ON, Canada, ²London Health Sciences Center, London, ON, Canada
*Purpose: The clinical relevance of ischemia-reperfusion injury (IRI) in the context of organ transplantation is well-established. IRI is associated with various forms of programmed cell…
2020 American Transplant Congress
Bone Marrow Mesenchymal Stem Cells Improve Rat Islet Angiogenesis by Upregulating of ISL1 and VEGF-A
Y. Wang¹, J. Wang², X. Ding²
¹Department of Renal Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, Xi’an, China, ²Department of Renal Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
*Purpose: Islet transplantation is an important therapy to cure diabetes, but poor angiogenesis in the early stage of islet transplantation is one of the main…
2020 American Transplant Congress
Inflammatory Cytokines Have a Different Effect from One HLA-II Subclass to Another on Human Endothelium
S. Beland, F. Bouchard-Boivin, A. Gama, O. Desy, S. A. De Serres
Universite Laval, Quebec, QC, Canada
*Purpose: Development of de novo donor-specific antibodies (DSAs) is associated with antibody-mediated rejection and allograft failure. More particularly, HLA-II DSAs, especially those against DQ are…
2020 American Transplant Congress
Novel Technique to Analyze Blood-Endothelial Cell Interactions Under Flow Conditions
M. R. Connolly¹, K. Petitpas¹, L. Burdorf¹, B. Cerel¹, C. Laird², D. Harris², A. Azimzadeh¹, R. N. Pierson III¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²University of Maryland, Baltimore, Baltimore, MD
*Purpose: The Bioflux system has been used to study platelet-driven thrombosis and leukocyte adhesion. Typically, whole blood is stained with calcein (green) to evaluate platelet…
2020 American Transplant Congress
Autophagy Attenuates Renal Allograft Interstitial Fibrosis by Regulating the EndMT Induced by TNF-α
Z. Gui, C. Suo, Z. Wang, S. Fei, Z. Han, J. Tao, H. Chen, L. Sun, X. Ju, R. Tan, M. Gu
Jiangsu Province Hospital, Nanjing, China
*Purpose: Kidney interstitial fibrosis is a significant cause of allograft failure in kidney transplantation. Autophagy is a self-digestive process that eliminates impaired or aged proteins…
2020 American Transplant Congress
Src Induces Endothelial-to-Mesenchymal Transition and Renal Allograft Fibrosis via Promoting WNT/β-catenin Pathway
W. Zijie, Z. Gui, M. Zheng, Z. Han, J. Tao, H. Chen, L. Sun, X. Ju, R. Tan, M. Gu
Jiangsu Province Hospital, Nanjing, China
*Purpose: Endothelial-to-mesenchymal(EndMT)is recognized as an important source of myofibroblasts contributing to the pathogenesis of allograft interstitial fibrosis in our previous studies. Increased proto-oncogene tyrosine-protein kinase…
2020 American Transplant Congress
Treg-Lymphatic Interfaces: Unique Molecular Structures for Cell-Cell Interactions During Migration
C. M. Paluskievicz, W. Piao, Y. Xiong, J. S. Bromberg
U of Maryland, Baltimore, MD
*Purpose: To promote transplant tolerance, T regulatory cells (Treg) must enter the afferent lymphatic vasculature by migrating across lymphatic endothelial cells (LECs), and then home…