Date: Tuesday, May 5, 2015
Session Name: Poster Session D: Innate Immunity in Transplantation
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Background: Delayed graft function (DGF) independently predicts reduced 5 yr kidney transplant survival. Treatments of DGF are lacking. Cold ischemia (CI) is a significant risk factor for DGF but the mechanism by which CI leads to DGF is unknown. The aim of this study was to determine the effects of CI on donor kidneys alone versus CI followed by warm reperfusion after kidney transplant (CI+Txp). We hypothesized that CI alone would produce a different injury phenotype to CI+Txp.
Methods: Male C57BL6 mice aged 8-12 weeks, were subjected to mouse kidney transplant. Donor kidneys were subjected to 3 hours CI in UW solution, and processed immediately or subjected to syngeneic mouse kidney transplant. Renal function was assessed by serum creatinine. Renal tubular cell (RTC) apoptosis and necrosis were quantified by an independent nephropathologist. TLR4, RIP3, cleaved BID, cleaved caspase-8 (CC8) and cleaved caspase-3 (CC3) were examined by immunoblot.
Results: CI+ Txp resulted in a significantly increased serum creatinine versus transplant without CI (Txp alone) (n=3; *p<0.05 vs. Txp alone). CI alone results in increased RTC apoptosis and CC3 but did not result in necrosis. In contrast, CI +Txp led to; (1) increased apoptotic factors CC8, cleaved BID and CC3, and thus increased RTC apoptosis; (2) increased RTC necrosis that was associated with increased RIP3 and TLR4.
|Control||Donor Kidney (CI)||Transplant Kidney (CI+ Txp)|
To cite this abstract in AMA style:Jain S, Plenter R, Nydam T, Ruller C, Edelstein C, Jani A. Phenotype of Renal Tubular Cell Death During Delayed Graft Function [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/phenotype-of-renal-tubular-cell-death-during-delayed-graft-function/. Accessed February 24, 2021.
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