Date: Sunday, May 3, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
EMT plays the principal role in the pathogenesis of IF/TA as a consequence in the failure of a transplanted kidney. Many facts suggest that the EMT can be a useful biomarker of chronic allograft dysfunction, the aim of our study was analysis of markers visualization and morphometrical evaluation. The study was approved by the Bioethics Committee. 54 recipients underwent renal biopsy (biopsies 0, 12, 36 mths ktx). Slides from renal biopsies were scanned using Axio Scan. Immunohistochemistry was performed on slides using monoclonal antibodies: cytokeratin, E-cadherin, vimentin. Antibodies binding was detected with Envision System. Using Lucia and NetBeans softwares IF/TA were evaluated for morphometrical parameters. Results of morphometric analysis evidenced a progressive IF/TA. This was found by decrease in diameter, perimeter and area of renal tubules, and increase of interstitial area. In I stadium values of tubules area, diameter, and perimeter were 3631,10 um2, 22,78 um and 94,10 um. In II, III stadium parameters were lower (area= 72,76 um2, diameter = 9,13 um, perimeter = 34,97 um). Morphometry of interstitial area showed increase from 44,55 um2 to 165,25 um2 in II, III stadium. Immunohistochemistry showed that average percent of positive stained tubules was 4.16% (cytokerat), 1.43% (E-cadh) and 1.61% (vim) in 0 mths ktx, 8.57% (cytokerat), 8.34% (E-cadh) and 2.11% (vim) 12 mths ktx, and 1.99% (cytokerat), 1.21% (E-cadh) and 0.43% (vim) 24 mths after ktx. Preliminary results of study showed that the highest expression of markers was in the biopsies after 12 mths after ktx. The study showed a relation between the expression intensity of tested markers of EMT. Morphometric analysis of IF/TA confirmed changes of parameters and was accurate tool for determination renal injury.
To cite this abstract in AMA style:Świder R, Perkowska-Ptasińska A, Stachurska A, Fabijańska-Mitek J, Zagożdżon R, Gruca S, Gołąb J, Durlik M. Morphometry of the Epithelial-Mesenchymal Transition (EMT) in Subsequent Biopsies from Transplanted Kidney [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/morphometry-of-the-epithelial-mesenchymal-transition-emt-in-subsequent-biopsies-from-transplanted-kidney/. Accessed April 15, 2021.
« Back to 2015 American Transplant Congress