ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Mitochondrial Membrane Potential Correlates with Ischemic Injury and Kidney Graft Function in Rats.

M. Chen, J. Huang, L. Qi, S. Kwon, R. Montgomery, Z. Sun.

Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.

Meeting: 2016 American Transplant Congress

Abstract number: C126

Keywords: Rat, Renal ischemia

Session Information

Date: Monday, June 13, 2016

Session Name: Poster Session C: Ischemia Reperfusion Injury and Organ Preservation

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

Related Abstracts
  • Can MitoQ Improve Renal and Mitochondrial Function Following Cold Storage and Transplantation?
  • Metabolic Dysregulation and Mitochondrial Dysfunction Are Key Features of Injury Profiles of Donor Kidneys After Brain Death.

Background

Both short- and long-term outcomes of all organ transplants depend on the quality of the organ. Presently there is no completely reliable method to evaluate the condition of organs removed from deceased donors before transplantation. Given the central role of mitochondria in executing normal cellular activities, we hypothesize that measurements of mitochondrial membrane potential (MMP) may correlate with ischemic injury and graft function.

Methods

Kidneys with or without warm ischemia from Lewis rats were harvested and preserved in cold UW solution. Mitochondria were isolated from kidney tissues at different cold preservation time points and MMP measurements were taken using the fluorescent JC-1 probe. To measure graft function, kidneys with extended cold ischemia time were transplanted into syngeneic Lewis recipients.

Results

MMP in kidneys without warm ischemia time remained at high levels (>4000 RFU/ug) for 72 hours following cold ischemia in UW solution. By contrast, MMP in kidneys with warm ischemia declined rapidly beginning 24 hours after cold ischemia in UW solution. In both models, decreased MMP correlated with graft non-function and animal death after transplantation.

Conclusion

Measurements of mitochondrial function that reflect overall tissue health could serve as a final common readout for all risk factors and injurious stimuli, and correlate with kidney graft function in the early post-operative period.

CITATION INFORMATION: Chen M, Huang J, Qi L, Kwon S, Montgomery R, Sun Z. Mitochondrial Membrane Potential Correlates with Ischemic Injury and Kidney Graft Function in Rats. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Chen M, Huang J, Qi L, Kwon S, Montgomery R, Sun Z. Mitochondrial Membrane Potential Correlates with Ischemic Injury and Kidney Graft Function in Rats. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/mitochondrial-membrane-potential-correlates-with-ischemic-injury-and-kidney-graft-function-in-rats/. Accessed March 6, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Role of Mitochondria, Metabolism and Inflammation: Pathways Influencing Short Term Outcomes Post-Renal Transplantation
    • Factors Influencing Creatinine Rise Following Donor Nephrectomy

    Visit Our Partner Sites

    American Transplant Congress (ATC)

    Visit the official site for the American Transplant Congress »

    American Journal of Transplantation

    The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

    American Society of Transplantation (AST)

    An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

    American Society of Transplant Surgeons (ASTS)

    The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

    Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

    Privacy Policy

    loading Cancel
    Post was not sent - check your email addresses!
    Email check failed, please try again
    Sorry, your blog cannot share posts by email.
    This site uses cookies: Find out more.