ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Metabolism Reprogramming During Conventional versus Ischemia-Free Liver Transplantation

Z. Guo1, Z. Zhang1, N. Gao1, H. Zhang2, X. He1

1Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China, 2Sun Yat-sen University, Guangzhou, China

Meeting: 2020 American Transplant Congress

Abstract number: B-343

Keywords: Graft function, Ischemia, Liver transplantation, Preservation

Session Information

Session Name: Poster Session B: Ischemia Reperfusion & Organ Rehabilitation

Session Type: Poster Session

Date: Saturday, May 30, 2020

Session Time: 3:15pm-4:00pm

 Presentation Time: 3:30pm-4:00pm

Location: Virtual

*Purpose: Our group has developed a novel transplant procedure called ischemia-free liver transplantation (IFLT), during which the liver can be procured, preserved and implanted without ischemia. In this study, metabolomics was used to explore the metabolic changes of liver allografts during conventional liver transplantation (CLT) and IFLT.

*Methods: Briefly, the IFLT procedure included the donor liver was harvested after an in situ NMP circuit was established, then moved into an organ reservoir for ex situ NMP, and finally implanted under in situ NMP. Liver biopsy samples were analyzed by untargeted LC-MS metabolomics method.

*Results: Volcano plot showed that there were a larger numbers of differentially expressed metabolites after graft re-vascularization in the CLT group than those in the IFLT group (up-regulated: 79 versus 42; down-regulated: 67 versus 41) (Fig. 1). Distinct metabolome profiles, particularly the pentose phosphate pathway (PPP), were identified between the two groups and between samples collected at the end of preservation and after graft re-vascularization. Nine of the differentially expressed metabolites obtained by comparison of IFLT_EP and CLT_EP belong to this pathway (Fig. 2). Besides, the levels of several differentially expressed metabolites, such as Guanosine 5’−monophosphate (GMP), D−Glucono−1,5−lactone, N6−methyladenosine were found to be associated with post-transplant graft function (Fig. 3).

*Conclusions: Compared with CLT grafts, the metabolic profiling of IFLT grafts is quite different. Obvious metabolic reprogramming, particularly changes of PPP occurred during CLT. The results of this study provide insights in understanding the mechanism of ischemia-reperfusion injury during liver transplantation.

 border=

 border=

 border=

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Guo Z, Zhang Z, Gao N, Zhang H, He X. Metabolism Reprogramming During Conventional versus Ischemia-Free Liver Transplantation [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/metabolism-reprogramming-during-conventional-versus-ischemia-free-liver-transplantation/. Accessed May 10, 2025.

« Back to 2020 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences