ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Life Supporting Orthotopic Cardiac Xenotransplantation Using Multiple Human Gene Expressing Pigs in Non Human Primates

A. K. Singh1, C. Goerlich1, T. Zhang1, I. Tatarov1, P. Odonkor1, E. Strauss1, B. Williams1, B. Lewis1, A. Hershfeld1, F. Sentz1, D. Ayares2, D. Kaczorowski1, B. Griffith1, M. Mohiuddin1

1Department of Surgery, University of Maryland, Baltimore, MD, 2Revivicor Inc, Blacksburg, WV

Meeting: 2022 American Transplant Congress

Abstract number: 84

Keywords: Graft survival, Heart/lung transplantation, Preclinical trails

Topic: Basic Science » Basic Science » 13 - Xenotransplantation

Session Information

Session Name: Xenotransplantation

Session Type: Rapid Fire Oral Abstract

Date: Sunday, June 5, 2022

Session Time: 3:30pm-5:00pm

 Presentation Time: 4:40pm-4:50pm

Location: Hynes Room 302

*Purpose: Cardiac xenotransplantation (CXTx) is proposed as an alternate treatment for end-stage cardiac failure patients. The prolonged survival of a heterotopic genetically engineered (GE) pig heart is well-established. Here we demonstrate the successful life-supporting orthotopic CXTx (OHTx) survival from various GE donor pigs.

*Methods: Orthotopic cardiac xenotransplantation (OHTx) from GE donor pig was performed in a 3-4-year-old baboon (n=10). GE donor pigs were divided into 4 groups based on the gene modifications (Table 1). Immunosuppression consisted of targeted T and B cell depletion along with costimulation blockade with anti-CD40 mAb. Cardioplegia was performed using the combination of DelNido solution or steen solution and recipient blood. A modified Shumway biatrial technique was used to perform heart transplantation. The total average ischemic time was between 90- 120 minutes as the heart was re-perfused after left atrial and aortic anastomoses were completed.

*Results: Life-supporting OHTx was performed successfully and the recipient with GE donor pig heart survived from 30 days to up to 264 days (median 100 days). The use of a heart preservation solution (either blood cardioplegia or Steen solution), minimal ischemic time, and early extubation allowed rapid recovery of heart function in the recipient. The use of a larger baboon (>20Kg) eliminated the requirement of allogeneic blood during or immediately after cardiopulmonary bypass. Though the role of every single gene was not feasible, the additional genetic modifications seem to be helpful

*Conclusions: These experiments demonstrate that OHTx can be achieved beyond the early perioperative period which has previously been reported as a major hurdle in CXTx. The combined effect of multiple gene modifications appears to be helpful in overcoming the various mechanisms of xenograft rejection. Future studies are in progress to evaluate the remaining barriers and achieve longer life-supporting cardiac xenotransplantation.

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Singh AK, Goerlich C, Zhang T, Tatarov I, Odonkor P, Strauss E, Williams B, Lewis B, Hershfeld A, Sentz F, Ayares D, Kaczorowski D, Griffith B, Mohiuddin M. Life Supporting Orthotopic Cardiac Xenotransplantation Using Multiple Human Gene Expressing Pigs in Non Human Primates [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/life-supporting-orthotopic-cardiac-xenotransplantation-using-multiple-human-gene-expressing-pigs-in-non-human-primates/. Accessed May 28, 2025.

« Back to 2022 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences