Date: Saturday, June 2, 2018
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall 4EF
Neutrophils can promote graft inflammation through a form of programmed cell death termed "NETosis". How alloantibodies contribute to neutrophil responses in grafts is not clear neither the expression of NETosis in peripheral blood of KTR. Aim and Methods: The purpose of this exploratory study was to evaluate NETosis in peripheral blood from adult kidney transplant recipients (KTR) with biopsy confirmed acute antibody mediated rejection (AAMR; n=10) compared with KTR without any immunologic event (IE) (controls, n=13). Spontaneous and LPS-induced NETosis was evaluated by fluorescence spectrometry with the Sytox Green staining and with confocal microscopy. The amount of NETs was quantified as the number of the fibrillar structures in which chromatin and MPO colocalized, divided by the number of cells in 6 high power fields per experimental condition. The expression of myeloperoxidase and Histone 3C (H3C) was measured by the MFI normalized for the area of every NET. Results. The main findings are depicted in Table 1.
|Total||AAMR (n=10)||Controls (n=13)||P value|
|Nets/cells ratio LPS (SD)||0.320 (0.23)||0.287 (0.19)||0.345 (0.27)||0.569|
|Nets/cells ratio spontaneous (SD)||0.368 (0.21)||0.374 (0.20)||0.364 (0.23)||0.912|
|MPO MFI LPS (SD)||1484 (364)||1461 (393)||1502 (354)||0.795|
|MPO MFI Spontaneous (SD)||1398 (348)||1301 (285)||1473 (383)||0.250|
|H3C MFI LPS (SD)||214 (129)||288 (156)||156 (64)||0.028|
|H3C MFI Spontaneous (SD)||195 (110)||216 (110)||179 (112)||0.451|
|NETs by fluorescence spectrometry LPS (SD)||1779 (611)||1739 (601)||1810 (641)||0.788|
|NETs by fluorescence spectrometry Spontaneous (SD)||1821 (673)||1952 (667)||1721 (687)||0.427|
Figure 1: Representative image of NETs by confocal microscopy.Conclusion: NETosis was observed similarly in both groups. NETosis may translate persistent activation of neutrophils despite inmunosuppression.
CITATION INFORMATION: Villca-Gonzales R., Torres-Ruiz J., Gomez-Martin D., Alberu J., Morales-Buenrostro L. Is the NETos Increased in Acute Antibody Mediated Rejection? Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Villca-Gonzales R, Torres-Ruiz J, Gomez-Martin D, Alberu J, Morales-Buenrostro L. Is the NETos Increased in Acute Antibody Mediated Rejection? [abstract]. https://atcmeetingabstracts.com/abstract/is-the-netos-increased-in-acute-antibody-mediated-rejection/. Accessed October 22, 2019.
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