Date: Saturday, April 29, 2017
Session Name: Poster Session A: Living Donor Kidney Transplant I
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall D1
Background. In kidney transplant recipients, IgG3 and IgG4 DSA have been associated with shorter time to acute AMR and with later allograft injury (glomerulopathy & IFTA), respectively. We were interested to determine the IgG subtypes circulating in a group of 46 adult (age and gender) potential kidney transplant recipients (PTR) with DSA to their potential living donors. All of them were CDC CxM –ive and FxM +ve or –ive. Methods. Class I and Class II HLA-DSA were determined by Luminex SAB (≥500 MFI = +ive). DSA IgG 1-4 subclasses were evaluated using monoclonal antibodies specific for IgG subclasses, as previously reported. All sera samples were tested the same day using cryopreserved (-70 0 C) sera from PTR containing DSAs against their respective donors. Results. Table 1 displays relevant findings of IgG subclasses and DSAs according to the FxM result.
|FxM +ive (n= 17)||FxM –ive (n=29)||P|
|DSA CI, n (%)||11 (64.7)||18 (62.1)||0.85|
|iDSA CI MFI||4444 (1482-8,532)||1,482 (954-2,836)||< 0.01|
|DSA CII, n (%)||12 (70.6)||20 (68.9)||0.91|
|iDSA CII MFI||3047 (1203 – 11426)||1511 (1170 – 2836)||0.22|
|IgG1 DSA, n (%)||10 (58.8)||4 (13.7)||< 0.01|
|IgG2 DSA, n (%)||3 (17.6)||3 (10.3)||0.65|
|IgG3 DSA, n (%)||1 (5.8)||0||0.37|
|IgG4 DSA, n (%)||1 (5.8)||1 (3.4)||1.0|
Figure 1 shows the relation between MFI level (DSA) and IgG subclasses grouped (+ive or –ive). A total of 86 DSA were detected, only 23 of them (26.7 %) were IgG subclasses identified, which highly correlates with DSA MFI.
Conclusions. A lower number of IgG subclasses were identified compared to the frequency reported in AMR patients. It is tempting to speculate that the absence of continuous antigenic stimulus is responsible for a lower level of detection of the IgG subclasses.
CITATION INFORMATION: Contreras A, Casillas A, Llorente L, Lima G, Arvizu A, De Santiago A, Vilatoba M, Alberu J, Arreola-Guerra J. IgG Antibody Subclasses in Potential Renal Transplant Recipients with DSA, AHG-CDC CxM Negative and FxM Positive or Negative with Their Potential Donors. Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Contreras A, Casillas A, Llorente L, Lima G, Arvizu A, Santiago ADe, Vilatoba M, Alberu J, Arreola-Guerra J. IgG Antibody Subclasses in Potential Renal Transplant Recipients with DSA, AHG-CDC CxM Negative and FxM Positive or Negative with Their Potential Donors. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/igg-antibody-subclasses-in-potential-renal-transplant-recipients-with-dsa-ahg-cdc-cxm-negative-and-fxm-positive-or-negative-with-their-potential-donors/. Accessed December 5, 2020.
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