Articles tagged "Flowcytometry crossmatching"

2020 American Transplant Congress
Outcomes of Post-Transplant Desensitization in High Immunological Risk Deceased Donor Kidney Transplant Recipients
D. Kumar, C. Song, L. Kamal, I. Moinuddin, M. Levy, C. Bhati, A. King, P. Kimball, G. Gupta
Virginia Commonwealth University, Richmond, VA
*Purpose: The kidney allocation system (KAS) was changed in 2015 to increase access to transplants for highly sensitized patients. However, recent data suggests that even…
2020 American Transplant Congress
The Economic Impact of National Standardization of Virtual Crossmatch Usage in Kidney Transplantation
J. E. Stanton¹, L. M. Rebellato², H. Pournik¹, J. E. Tuttle-Newhall¹, H. Jones¹, W. Irish¹, D. B. Leeser¹
¹Department of Surgery, East Carolina University, The Brody School of Medicine, Greenville, NC, ²Department of Pathology, East Carolina University, The Brody School of Medicine, Greenville, NC
*Purpose: The implementation of virtual crossmatching (VXM) into conventional protocols may reduce expenditures associated with organ allocation.*Methods: A single center retrospective analysis of the total…
2020 American Transplant Congress
Preformed Donor Specific Antibody and Mfi-Dependent Effects in Simultaneous Liver-Kidney Transplantation
V. Fleetwood, C. Papageorge, D. Aufhauser, G. Leverson, B. Welch, L. Hidalgo, D. Foley, Y. Yankol, A. D'Alessandro, D. Al-Adra, R. Redfield, J. Mezrich
Department of Transplantation, University of Wisconsin, Madison, WI
*Purpose: Preformed donor specific antibody to HLA Class II (pDSA) is suggested to be an independent risk factor for mortality in simultaneous liver and kidney…
2020 American Transplant Congress
Influence of Human Leukocyte Antigen Status and Gender of Positive Crossmatch Patients on Long-Term Survival Outcomes of Antibody Incompatible Renal Transplantation
N. R. Macha¹, A. Abimbola¹, N. Khovanova², D. Briggs³, N. Krishnan⁴
¹CIRAL, Coventry University, Coventry, United Kingdom, ²School of Engineering, University of Warwick, Warwickshire, United Kingdom, ³Director, Tissue Typing Lab, NHSBT, Birmingham, United Kingdom, ⁴Consultant Transplant Nephrologist and Visiting Professor, CIRAL, University Hospital Coventry & Warwickshire, Coventry, United Kingdom
*Purpose: Human Leukocyte Antigen (HLA) sensitization is a significant obstacle which limits the use of renal transplantation, but it serves as a therapeutic option for…
2020 American Transplant Congress
Assessment of Rapid Optimized Flow Cytometric Crossmatch (FCXM) Assay with Luminex Class I Single Antibody Test
J. Yoo¹, S. Lee², J. Jang³, A. Choi², E. Oh¹
¹Department of Laboratory Medicine, Catholic University of Korea, Seoul, Korea, Republic of, ²Department of Laboratory Medicine, Seoul St. Mary's Hospital, Seoul, Korea, Republic of, ³Department of Biomedical Science, Graduate School, Catholic University of Korea, Seoul, Korea, Republic of
*Purpose: Flow cytometric crossmatch test (FCXM) is critical to detect donor-specific antibodies (DSA) in transplant patients. Rapid, simple and sensitive FCXM procedure can improve HLA…
2019 American Transplant Congress
Impact Of Delayed Kidney Transplantation On Positive Crossmatch Liver Kidney Transplantation
Z. Rokop, C. Kubal, B. Ekser, R. Mangus, J. Fridell, P. Mihaylov, W. Goggins
Department of Surgery, Indiana University Hospital, Indianapolis, IN
*Purpose: Kidney transplantation in sensitized patients is associated with an increased immunological risk. Significant decrease in circulating anti-HLA donor specific antibodies (DSA) has been observed…
2019 American Transplant Congress
The Effect Of Exclusive Virtual Crossmatch To Allocate Deceased Donor Kidney Transplant On Cold Ischemia Time And Acute Rejection
E. Abuhelaiqa¹, M. Alkadi¹, S. Thappy¹, S. Saleh², J. Mahmoud³, M. Jarman³, O. Fituri¹, M. Elkhalifa⁴, J. Wadström³, H. Al-Malki¹
¹Medicine-Nephrology, Hamad Medical Corporation, Doha, Qatar, ²Histocompatibility & Immunogenetics Laboratory,, Hamad Medical Corporation, Doha, Qatar, ³Surgery-Transplant, Hamad Medical Corporation, Doha, Qatar, ⁴Histocompatibility & Immunogenetics Laboratory, Hamad Medical Corporation, Doha, Qatar
*Purpose: Virtual crossmatch (VXM) using Luminex single antigen bead has significantly improved prediction of an actual negative crossmatch. A negative crossmatch is typically required for…
2019 American Transplant Congress
Patients with Immunological Diseases or on Peritoneal Dialysis Are Prone to False Positive Flow Cytometry Cross Match
Q. Xu¹, A. House², S. Leckie¹, L. Gunaratnam², P. Luke³, A. Jevnikar²
¹Pathology and Lab Medicine, London Health Science Center, London, ON, Canada, ²Medicine, Western University, London, ON, Canada, ³Surgery, Western University, London, ON, Canada
*Purpose: Despite implementation of virtual cross matches, flow cytometry cross matches (FCXM) are still used by many transplant centers to determine immunological risk prior to…
2019 American Transplant Congress
Targeted Epitope Mutagenesis as a Strategy to Decrease Xenoantibody Binding
J. Ladowski, G. Martens, Z. Wang, L. Reyes, M. Tector, J. Tector
University of Alabama-Birmingham, Birmingham, AL
*Purpose: Xenotransplantation, the use of genetically-modified pig organs for transplantation, has been limited by antibody-mediated rejection. The generation of a three-antigen knockout pig provided a…
2019 American Transplant Congress
α -1,3-galactosyltransferase Knockout Porcine Tissue And Organ May Reduce The Risks Of Antibody Mediated Rejection Via Less Rhesus Igm Cross-reactivity.
J. Yoon, B. Ezekian, M. Manook, J. Kwun, S. Knechtle
Duke Transplant Center, Durham, NC
*Purpose: Previously, we observed that sera from rhesus macaques contained preformed antibodies against wild type (WT) swine splenocytes. In the context of xenotransplantation, preformed xenoantibodies…