ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

IE-1 Specific T Cell Receptor Repertoire Diversity and Protection against CMV Reactivation

M. Lucia Perez1, F. Rubelt1, S. Busque1, C. O. Esquivel1, S. M. Krams1, O. Bestard2, O. M. Martinez1

1Surgery, Stanford University School of Medicine, Stanford, CA, 2Nephrology, University of Barcelona, L'Hospitalet de Llobregat, Spain

Meeting: 2019 American Transplant Congress

Abstract number: A134

Keywords: Cytomeglovirus, T cell receptors (TcR)

Session Information

Date: Saturday, June 1, 2019

Session Name: Poster Session A: Biomarkers, Immune Monitoring and Outcomes

Session Time: 5:30pm-7:30pm

 Presentation Time: 5:30pm-7:30pm

Location: Hall C & D

Related Abstracts
  • Identifying Specificity Groups in the CMV IE-1 T Cell Receptor Repertoire
  • Elderly ESRD Patients Have a Significantly Narrowed T-Cell Receptor Repertoire Diversity

*Purpose: Cytomegalovirus (CMV) is a major complication for organ transplant recipients. T cells reacting to the CMV antigen immediate-early (IE-1) are key in controlling reactivation of the virus after the transplant. However, our understanding of the CMV-specific T cell response is incomplete and the characteristics of the TCR repertoire conferring protection to CMV are not known. The aim of this study is to identify protective specificity groups in the IE-1-specific TCR repertoire pretransplantation in A02:01 kidney transplant patients.

*Methods: PBMC from three healthy donors and six kidney transplant recipients that are CMV seropositive, IE-1-ELISPOT+, and HLA A02:01 were stimulated in vitro with IE-1 peptides. After 24h the IE1-specific CD8+ T cells were single cell FACS-sorted based on the expression of the CD137 activation marker. We sequenced the IE-1-specific TCRαβ chains, defined the IE-1-TCR repertoire clonality for each patient and analysed the IE-1 TCRβ sequences using the GLIPH biocomputational clustering algorithm.

*Results: The IE-1-TCR repertoire analysis revealed clonally expanded T cell populations in both the healthy seropositive donors and kidney transplant patients. Interestingly, the IE1-TCR repertoire of patients who did not have CMV reactivation was more diverse prior to transplant than that of the patients who did have CMV-reactivation post-transplant (Gini index: 0.2 vs 0.7, respectively). Analysis of post-transplant CMV-specific T cells revealed a decrease of IE-1-TCR diversity in the group of ‘non- reactivating’ patients to similar levels than that of reactivating patients. To note, most immunodominant T cell clones detected prior to transplantation were also detected 6 months post-transplant in both CMV reactivating and CMV non-reactivating patients.GLIPH identified multiple clusters of different TCRβ sequences putatively binding the same IE-1 epitope as a “public” IE-1 TCR sequence reported in the literature as highly conserved among the HLA A2:01 CMV-seropositive population.

*Conclusions: In summary, a single cell TCR sequencing and computational approach has the potential to identify and cluster TCR sequences in transplant patients that control CMV replication and facilitate prediction of CMV risk in the transplant setting.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Perez MLucia, Rubelt F, Busque S, Esquivel CO, Krams SM, Bestard O, Martinez OM. IE-1 Specific T Cell Receptor Repertoire Diversity and Protection against CMV Reactivation [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/ie-1-specific-t-cell-receptor-repertoire-diversity-and-protection-against-cmv-reactivation/. Accessed January 16, 2021.

« Back to 2019 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.