Articles tagged "T cell receptors (TcR)"

2020 American Transplant Congress
A Proof-of-Principle Study to Evaluate Autologous Hematopoietic Stem Cell Transplantation for Allogeneic Organ Transplant Tolerance (ASCOTT)
A. Chruscinski¹, S. Juvet¹, S. Moshkelgosha¹, E. Renner¹, L. Lilly¹, N. Selzner¹, C. Bredeson², O. Adeyi¹, S. Fischer¹, A. Demetris³, J. Zhang¹, M. Epstein¹, A. Clement², K. Khalili¹, Z. Galvin¹, A. Humar¹, T. Kim¹, K. Tinckham¹, T. Yi¹, G. Levy¹, H. Atkins²
¹Multi-Organ Transplantation, University Health Network, Toronto, ON, Canada, ²The Ottawa Hospital, Ottawa, ON, Canada, ³University of Pittsburgh, Pittsburgh, PA
*Purpose: Long-term survival of recipients of solid organ transplants is limited by chronic rejection, disease recurrence and immunosuppression-induced toxicity. Self-tolerance is established after autologous hematopoietic…
2020 American Transplant Congress
T-cell Receptor Deep Sequencing to Distinguish Autoimmune Recurrence from Allograft Rejection in Liver Transplant Patients with Autoimmune Liver Disease
K. J. Yang¹, M. Martinez², B. Shonts¹, C. A. Parks¹, A. Z. Obradovic¹, T. M. Savage¹, E. Spyrou¹, S. Lau¹, M. I. Colquhoun¹, K. Rodgers¹, E. Verna³, S. M. Coley⁴, S. Yang¹, Y. Shen⁵, M. Sykes¹
¹Columbia Center for Translational Immunology, Department of Medicine, Columbia University, New York, NY, ²Department of Pediatrics, Columbia University, New York, NY, ³Department of Medicine, Columbia University, New York, NY, ⁴Department of Pathology, Columbia University, New York, NY, ⁵Center for Computational Biology & Bioinformatics, Department of Systems Biology, Columbia University, New York, NY
*Purpose: Patients who undergo liver transplantation for autoimmune liver disease (ALD) experience high rates of graft failure. The relative roles of recurrent ALD and rejection…
2020 American Transplant Congress
MHC II-Decoy Car-T Cells to Induce Long-Term Allotransplant Organ Tolerance
W. McCall¹, T. Baybutt¹, A. Snook¹, A. Shah²
¹Department of Pharmacology and Experimental Therapeutics, Sidney Kimmel Medical College, Philadelphia, PA, ²Department of Surgery, Division of Transplantation, Sidney Kimmel Medical College, Philadelphia, PA
*Purpose: Allotransplantation represents the definitive therapy for end-stage renal, heart, liver, and lung disease. Current immunosuppressive regimens fail to promote long-term engraftment, leading to chronic…
2020 American Transplant Congress
T Cells from Highly Sensitized Kidney Transplant Patients Display Distinct TCR Repertoires
S. Negi¹, M. Li¹, A. Rutman², S. Paraskevas¹, C. Polychronakos¹, J. Tchervenkov¹
¹RIMUHC, Montreal, QC, Canada, ²McGill University, Montreal, QC, Canada
*Purpose: Sensitization to HLA antigens limits patient’s access to renal transplantation and causes higher risk of acute rejection and graft loss. T-cells, B-cells and donor-specific…
2020 American Transplant Congress
A Single-Cell Transcriptome Atlas of 15 Adult Human Organs
Z. Guo¹, S. He², L. Wang¹, Y. Liu², J. Xu¹, J. Bei², X. He¹
¹Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China, ²The Cancer Centre of Sun Yat-Sen University, Guangzhou, China
*Purpose: The transcriptome diversity of cell types within and across tissues is of great importance for biological function of human beings. Here we conducted a…
2020 American Transplant Congress
Pregnancy Alloimmunization Increases the Clonality of the Endogenous Treg Repertoire
K. Krupp, E. Lewis, R. Xu, P. Porrett
University of Pennsylvania, Philadelphia, PA
*Purpose: Pregnancy is a common immune sensitizing event, which creates an access barrier to organ transplantation and can impact transplant outcomes. While most research has…
2019 American Transplant Congress
Integrative Analysis of Immune Repertoire and Gene Expression from RNA-seq in Kidney Organ Transplants
S. Pineda¹, A. Ravoor², O. Bestard³, M. Sarwal¹, M. Sirota²
¹Department of Surgery, UCSF, San Francisco, CA, ²BCHSI, UCSF, San Francisco, CA, ³Department of Surgery, 3Bellvitge University Hospital, Barcelona, Spain
*Purpose: The main problem with kidney transplantation is the possibility of rejection, which is classified as either antibody-mediated rejection (AMR) or T-cell-mediated rejection (TCMR) depending…
2019 American Transplant Congress
IE-1 Specific T Cell Receptor Repertoire Diversity and Protection against CMV Reactivation
M. Lucia Perez¹, F. Rubelt¹, S. Busque¹, C. O. Esquivel¹, S. M. Krams¹, O. Bestard², O. M. Martinez¹
¹Surgery, Stanford University School of Medicine, Stanford, CA, ²Nephrology, University of Barcelona, L'Hospitalet de Llobregat, Spain
*Purpose: Cytomegalovirus (CMV) is a major complication for organ transplant recipients. T cells reacting to the CMV antigen immediate-early (IE-1) are key in controlling reactivation…
2019 American Transplant Congress
TCR Transgenes to Understand Human Xenotolerance Induction
G. Nauman, C. Borsotti, N. Danzl, M. Khosravi-Maharlooei, H. Li, E. Chavez, Y. Yang, M. Sykes
Columbia Center for Translational Immunology, New York, NY
*Purpose: Swine thymus transplantation is a promising approach to clinical tolerance for pig-to-human xenotransplantation. In humanized mice, a swine thymus graft supports de novo selection…
2019 American Transplant Congress
Lymphotoxin Signaling Regulates T Cell Lymphatic Trafficking
W. Piao¹, L. Li², V. Saxena², Y. Xiong², M. WillsonShirkey², C. Paluskievicz², J. Bromberg³
¹Microbiology and Immunology, U. Maryland, Baltimore, baltimore, MD, ²Surgery, U. Maryland, Baltimore, Baltimore, MD, ³Microbiology and Immunology, U. Maryland, Baltimore, Baltimore, MD
*Purpose: Lymphotoxin (LT) α1β2 is crucial for lymphatic organ development and orchestration of immune responses. LTα1β2 is preferentially expressed by regulatory T cells (Treg) for…