ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Identification of miRNAs Associated With Induced Tolerance to Allografts

M. Vitalone, L. Wei, C. Esquivel, S. Busque, O. Martinez, S. Krams.

Surgery-Transplantation, Stanford University School of Medicine, Stanford, CA.

Meeting: 2015 American Transplant Congress

Abstract number: C248

Keywords: Liver transplantation, Rat, Tolerance

Session Information

Session Name: Poster Session C: Translational Biomarkers and Immune Monitoring

Session Type: Poster Session

Date: Monday, May 4, 2015

Session Time: 5:30pm-6:30pm

 Presentation Time: 5:30pm-6:30pm

Location: Exhibit Hall E

Introduction: Although the liver is less immunogenic than other solid organs, most liver transplant recipients receive lifelong immunosuppression. Total lymphoid irradiation (TLI) is part of a protocol that is currently being used in the clinic to induce tolerance to renal allografts. Our goal was to identify the miRNAs of tolerant graft recipients.in an experimental model of TLI-induced tolerance. Methods: To identify the miRNAs associated with TLI-induced tolerance we examined syngeneic (syn) recipients (DA into DA) and allogeneic (allo) recipients (DA into Lewis) of OLT that received post-transplant TLI, allo-recipients (DA into Lewis) that were not treated post-transplant, and normal DA rats. Untreated allo-recipients reject their grafts within 10 days whereas TLI treated recipients have long-term graft survival (>100 days) thus miRNAs were examined at two time points, seven days (d7) post-transplant (allo-untreated, allo-TLI, syn-TLI and at 100 days (d100) post-transplant (allo-TLI), using Taqman array cards. Results: Through unsupervised hierarchical clustering, two main groups emerged, allo-livers obtained on d7 irrespective of TLI treatment and syn-livers clustered with allo-TLI d100 livers. Similarly, principal component analysis demonstrated the tolerant allo-TLI livers (d100) were closely related to the syn-liver grafts. In addition, several miRNAs, including miR142-3p, miR142-5p, miR147, miR181a, miR184 and miR342-3p, demonstrated increased expression in tolerant livers (both at d7 and d100) as compared to syngeneic grafts, however these miRNAs are also increased during acute rejection. Two miRNAs, miR196b and miR499 are specifically increased in allografts with established tolerance (d100). Validation of these miRNAs and determination of target mRNAs are on-going. Conclusions: Our results suggest the miRNA profile of tolerant allografts is very similar to the profile observed in syngeneic grafts. There are however a few miRNAs that may be attractive biomarkers of TLI-induced tolerance and allograft stability

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Vitalone M, Wei L, Esquivel C, Busque S, Martinez O, Krams S. Identification of miRNAs Associated With Induced Tolerance to Allografts [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/identification-of-mirnas-associated-with-induced-tolerance-to-allografts/. Accessed May 9, 2025.

« Back to 2015 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences