ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Fecal Microbiota Transplant Results in Durable Changes to Host Microbiota That Correlate with Allograft Outcome.

C. Brinkman, L. Hittle, W. Fricke, E. Mongodin, J. Bromberg.

CVID and IGS, University of Maryland School of Medicine, Baltimore, MD.

Meeting: 2016 American Transplant Congress

Abstract number: 296

Keywords: Graft survival, Inflammation, Rejection

Session Information

Date: Monday, June 13, 2016

Session Name: Concurrent Session: Challenges to Graft Survival and Tolerance: Animal Models

Session Time: 4:30pm-6:00pm

 Presentation Time: 5:42pm-5:54pm

Location: Room 306

Related Abstracts
  • The Gut Microbiota Regulates Murine Cardiac Transplant Outcome
  • Role of Commensal Microbiota in Skin Allograft Rejection

Hypothesis: We previously presented highly significant differences in the bacterial community structures of human renal allograft recipients over time. Likewise, murine normal, colitic, and pregnant fecal samples influenced cardiac allograft survival and were markedly distinct in bacterial community structure, notably, in abundance of Bifidobacterium. We hypothesized that these differences persist after fecal microbiota transplant (FMT) into mice with cardiac allografts and correlate with allograft outcome.

Methods: Mice were fed antibiotics (kanamycin, gentamicin, colistin, metronidazole, vancomycin) ad libitum in drinking water on days -6 to -1 before transplant. On d0 C57BL/6 mice received BALB/c hearts. Fecal samples from healthy C57BL/6, female mice on d11 of pregnancy, colitic T cell receptor transgenic mice that spontaneously develop colitis, or cultured Bifidobacterium pseudolongum (ATCC25526) were transferred on d0 by gavage. Mice received daily immunosuppression tacrolimus (2 mg/kg/d sc d0-40 or 3 mg/kg/d sc d0-60) starting on day 0. Fecal pellets and intestinal tissue were collected from transplanted mice, and analyzed via 16S rRNA gene sequencing and RNA-Seq. Cardiac allografts were assessed for survival, harvested at d40, 60, or rejection, and stained with H&E and Masson's Trichrome.

Results: 16S rRNA gene analysis of transplant recipient samples revealed highly significant differences in the bacterial community structures of recipients of normal, colitic, and pregnant FMT, and cultured B. pseudolongum, as determined by bacterial composition and relative abundance, principle component analysis and hierarchical clustering. Bacteria from the genus Bifidobacterium were absent in colitic, yet present in normal and pregnant source samples and remained fairly abundant in pregnant transplanted samples for at least 40 days. In general the microbiota of recipients of normal, colitic, and pregnant fecal samples converged over time, but remained distinct for at least 40 days. Mice receiving pregnant FMT or B. pseudolongum had the highest graft survival rates, indicating a possible anti-inflammatory effect of Bifidobacterium bacteria.

Conclusion: FMT of pro- and anti-inflammatory fecal microbiota and specific components of the microbiota results in durable changes to host microbiota. These changes correlate with alterations in systemic immunity with consequences for graft survival, rejection, and inflammation.

CITATION INFORMATION: Brinkman C, Hittle L, Fricke W, Mongodin E, Bromberg J. Fecal Microbiota Transplant Results in Durable Changes to Host Microbiota That Correlate with Allograft Outcome. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Brinkman C, Hittle L, Fricke W, Mongodin E, Bromberg J. Fecal Microbiota Transplant Results in Durable Changes to Host Microbiota That Correlate with Allograft Outcome. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/fecal-microbiota-transplant-results-in-durable-changes-to-host-microbiota-that-correlate-with-allograft-outcome/. Accessed March 9, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.