Session Time: 6:00pm-7:00pm
Presentation Time: 6:05pm-6:10pm
*Purpose: The purpose is to investigate the functional role of eosinophils during hepatic ischemia and reperfusion injury (IRI).
*Methods: Mice with antibody-induced eosinophil depletion and two strains of mice with genetic deletion of eosinophils were be used in the studies.
*Results: Unexpectedly, we identified a rapid accumulation of eosinophils in human liver grafts following hepatic transplantation. In contrast, no eosinophils were detectable in healthy liver tissues. Studies with genetic models of eosinophil deficiency or antibody-mediated eosinophil depletion revealed exacerbated injury following hepatic ischemia and reperfusion. Adoptive transfer of bone marrow-derived eosinophils normalized liver injury of eosinophil-deficient mice and reduced hepatic ischemia and reperfusion injury in wild-type mice. Mechanistic studies combining genetic and adoptive transfer approaches identified a critical role of suppression of tumorigenicity (ST2)-dependent production of interleukin-13 by eosinophils in the hepatoprotection against ischemia reperfusion-induced injury.
*Conclusions: Taken together, the present studies uncovered a previously unrecognized hepatoprotective function of eosinophils and implicated the IL-33/ST2-dependent IL-13 production in mediating the protective effect. The findings support further exploration of eosinophils and IL-33/ST2 signaling as candidate therapeutic targets to improve the outcomes of liver surgery and transplantation.
To cite this abstract in AMA style:Yang Y, Wang Y, Wang M, Jeong J, Xu L, Wen Y, Emontzpohl C, Dar W, Ju C. Eosinophils Attenuate Hepatic Ischemia Reperfusion Injury in Mice Through St2-Dependent Il-13 Production [abstract]. Am J Transplant. 2021; 21 (suppl 3). https://atcmeetingabstracts.com/abstract/eosinophils-attenuate-hepatic-ischemia-reperfusion-injury-in-mice-through-st2-dependent-il-13-production/. Accessed June 12, 2021.
« Back to 2021 American Transplant Congress