ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Donor-Recipient HLA Mismatched Peptides Calculated by PIRCHE Algorithm Associate with De Novo Donor-Specific Cellular and Humoral Alloreactivity and Predicts Inferior Graft Outcome

M. Meneghini1, E. Crespo2, M. Niemann3, A. Torija2, E. Melilli4, M. Jarque2, N. Montero4, J. Cruzado1, J. Grinyo1, O. Bestard1

1Kidney Transplantation Unit, Bellvitge University Hospital- IDIBELL, L'Hospitalet de Llobregat. Barcelona, Spain, 2Experimental Nephrology Laboratory, IDIBELL, L'Hospitalet de Llobregat. Barcelona, Spain, 3PIRCHE AG, Berlin, Germany, 4Kidney Transplantation Unit, Bellvitge University Hospital, L'Hospitalet de Llobregat. Barcelona, Spain

Meeting: 2019 American Transplant Congress

Abstract number: B69

Keywords: Allorecognition, Histocompatibility, HLA antibodies, T cell activation

Session Information

Session Name: Poster Session B: Biomarkers, Immune Monitoring and Outcomes

Session Type: Poster Session

Date: Sunday, June 2, 2019

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Hall C & D

*Purpose: PIRCHE-II algorithm predicts mismatched donor-derived HLA peptides number presented on recipient HLAclass-II molecules to recipient T-cells (indirect pathway of allorecognition) and has been associated to de novo donor-specific antibodies (dnDSA) formation and graft outcome. Since indirect pathway of allorecognition plays a role on T-cell activation after Kidney Transplantation (KTx) we hypothesized that a high PIRCHE-II score would predict de novo activation of donor-specific (d-Sp)T-cell activation after KTx.

*Methods: In 266 consecutive KTx patients without preformed DSA, donor/recipient mismatch using PIRCHE-II algorithm was evaluated. De novo d-Sp T-cell alloimmunity by both direct and indirect pathways of allorecognition using IFN-y ELISPOT assay, dnDSA and graft outcomes were assessed after at least 24months of follow-up (69-30).

*Results: 16% of patients developed acute rejection (AR) during follow-up. PIRCHE-II score did not associate with AR but PIRCHE-II, DR and DQ scores were higher in patients developing antibody-mediated rejection (117±31vs74±35,p=0.008;20±13 vs11±9,p=0.04 and 30±10 vs18±14,p=0.07). Pre-KTx d-Sp T-cell alloreactivity was observed in 49% of patients, whereas 20% showed de novo d-Sp T-cell alloreactivity after KTx. Notably, patients with de novo d-Sp T-cell alloreactivity displayed higher PIRCHE-II and DR score compared to quiescent T-cell alloreactive patients (91±29 vs 67-28, p=0.03 and 16±7 vs 9±8, p=0.01). Furthermore, patients with de novo d-Sp T-cell alloreactivity as well as those with high PIRCHE-II score were at higher risk of developing dnDSA (HR=4.5,95%CI1.2-17,p=0.02;HR=1.012,95%CI1.003-1.02,p=0.009 for T-cell alloreactivity and PIRCHE-II, respectively) (Figure 1a,1b). Finally, a higher PIRCHE-II score independently predicted death-censored graft survival in a multivariate analysis (HR=5.3,95%IC=1.3-21,p=0.017).

*Conclusions: A poor donor/recipient HLA matching assessed by PIRCHE-II algorithm discriminates patients at higher risk of post-transplant d-Sp T-cell alloimmunity as well as those subsequently developing dnDSA and a poorer graft outcome.

 border=

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Meneghini M, Crespo E, Niemann M, Torija A, Melilli E, Jarque M, Montero N, Cruzado J, Grinyo J, Bestard O. Donor-Recipient HLA Mismatched Peptides Calculated by PIRCHE Algorithm Associate with De Novo Donor-Specific Cellular and Humoral Alloreactivity and Predicts Inferior Graft Outcome [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/donor-recipient-hla-mismatched-peptides-calculated-by-pirche-algorithm-associate-with-de-novo-donor-specific-cellular-and-humoral-alloreactivity-and-predicts-inferior-graft-outcome/. Accessed June 17, 2025.

« Back to 2019 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences