Articles tagged "Histocompatibility"

2020 American Transplant Congress
Calculated Panel Reactive Antibody Values Increase for Kidney Transplant Candidates with HLA-DQA and HLA-DPB Unacceptable Antigens
E. P. Kransdorf¹, C. Murphey², M. Maiers³, L. Gragert⁴
¹Cedars-Sinai Heart Institute, Beverly Hills, CA, ²Immunogenetics Lab, Southwest Immunodiagnostics, Inc., San Antonio, TX, ³Bioinformatics Research, Be The Match/National Marrow Donor Program, Minneapolis, MN, ⁴Department of Pathology, Tulane University School of Medicine, New Orleans, LA
*Purpose: Calculated panel-reactive antibody (CPRA) is the official metric of sensitization used by the United Network for Organ Sharing (UNOS) for kidney allocation. CPRA includes…
2020 American Transplant Congress
Are HLA-DQ De Novo Donor-Specific Antibodies a Risk Factor for Acute Humoral Rejection?
R. Hod Dvorai¹, C. A. Hubbell¹, M. R. Laftavi², R. Shahbazov², B. Gallay³, O. G. Pankewycz³
¹Pathology, SUNY Upstate Medical University, Syracuse, NY, ²Surgery, SUNY Upstate Medical University, Syracuse, NY, ³Medicine, SUNY Upstate Medical University, Syracuse, NY
*Purpose: To identify HLA-DQ de novo donor-specific antibodies (dnDSA) characteristics associated with acute humoral rejection (ABMR) and response to treatment.*Methods: We performed a retrospective analysis…
2020 American Transplant Congress
Impact of Catch-Up Vaccinations on Anti-HLA Antibody Responses in Pediatric Kidney Transplant Candidates
H. Sweiss¹, J. Lyons², R. Hall¹, K. Hitchman³, D. Ranch⁴, E. Kincaide¹, B. Crowther⁵
¹University Health System, San Antonio, The University of Texas Health Science Center at San Antonio, San Antonio, TX, ²Loyola University Medical Center, Chicago, IL, ³University Health System, San Antonio, TX, ⁴The University of Texas Health Science Center at San Antonio, San Antonio, TX, ⁵University of Colorado Health, Aurora, CO
*Purpose: Evaluate antibody (Ab) response after catch-up vaccinations in pediatric kidney transplant candidates (PKTC).*Methods: A single center retrospective review of PKTC less than or equal…
2020 American Transplant Congress
Eplet Mismatch Imputed from HLA Serotype-Level Data Can Improve Risk Stratification for Kidney Allograft Loss
A. Heinzel, R. Reindl-Schwaighofer, A. Kainz, K. Hu, R. Oberbauer
Department of Medicine III - Division of Nephrology and Dialysis, Medical University of Vienna, Vienna, Austria
*Purpose: HLA-Epitope mismatch improves risk stratification following kidney transplantation. Based on population data, high resolution HLA types required for epitope prediction can be imputed based…
2020 American Transplant Congress
A Multicenter Analysis of the Proportion and Pattern of Unexplained HLA Antibodies in Kidney Recipients without a History of Sensitization
J. Lan¹, J. S. Gill¹, H. M. Gebel², R. A. Bray², R. Liwski³, A. Vinson⁴, A. Greenshields³, S. Dobrer⁵, O. Gunther⁵, K. Sherwood⁵, F. H. Claas⁶, S. Heidt⁶, G. Karahan⁶, J. Gill⁷, M. Gill¹, U. Varyani¹, P. Jindal¹, P. Keown⁵
¹Medicine, Division of Nephrology, University of British Columbia, Vancouver, BC, Canada, ²Pathology, Emory University, Atlanta, GA, ³Pathology, Dalhousie University, Halifax, NS, Canada, ⁴Nephrology, Dalhousie University, Halifax, NS, Canada, ⁵Pathology, University of British Columbia, Vancouver, BC, Canada, ⁶Immunohaematology and Blood Transfusion, Leiden University Medical Center, Leiden, Netherlands, ⁷Nephrology, Emory University, Atlanta, GA
*Purpose: Previous studies report a high prevalence of unexplained antibody reactivities on the single antigen bead (SAB) test. In a contemporary multicenter cohort of non-sensitized…
2020 American Transplant Congress
HLA Specific Antibody Levels after EDTA Treatment in CDC Crossmatch Positive and Negative Sera with Known Donor-Specific Antibodies
J. Lauronen¹, T. Jaatinen¹, J. Holgersson², C. Naper³, J. Peräsaari¹
¹Histocompatibility Laboratory, Finnish Red Cross Blood Service, Helsinki, Finland, ²Department of Laboratory Medicine, Sahlgrenska Academy at University of Gothenburg and Sahlgrenska University Hospital, Gothenburg, Sweden, ³Histocompatibility Laboratory, Oslo University Hospital, Oslo, Norway
*Purpose: Ethylenediaminetetraacetic acid (EDTA) treatment of serum samples overcomes the prozone effect that may interfere with solid phase HLA specific antibody analyses. In patients with…
2020 American Transplant Congress
The Clinical Relevance of cPRA in 0-ABDRDQ Mismatched Kidney Transplants
J. H. Lan¹, D. Chang¹, M. Kadatz¹, J. Gill¹, H. M. Gebel², J. S. Gill¹
¹Medicine, Division of Nephrology, University of British Columbia, Vancouver, BC, Canada, ²Pathology, Emory University, Atlanta, GA
*Purpose: The clinical relevance of immune sensitization as measured by cPRA in kidney transplantation remains uncertain.*Methods: We identified 4,057 adult, first, kidney only transplant recipients…
2019 American Transplant Congress
Characterization of Circulating T-cell Specific Exosomes during Acute Rejection in Murine Heart Transplantation Model
R. Hu¹, V. Korutla¹, L. Korutla¹, S. Rostami¹, A. Habertheuer¹, S. Addya², J. Harmon¹, P. Zielinski¹, S. Reddy¹, A. Freas¹, C. Ram¹, A. Naji¹, P. Vallabhajosyula¹
¹Surgery, Hospital of the University of Pennsylvania, Philadelphia, PA, ²Thomas Jefferson University, Philadelphia, PA
*Purpose: Exosomes are nanoparticles (30-200nm) released by many cell types. They carry nucleic acid and proteins that may reflect conditional changes in their tissue counterparts.…
2019 American Transplant Congress
Donor-Recipient HLA Mismatched Peptides Calculated by PIRCHE Algorithm Associate with De Novo Donor-Specific Cellular and Humoral Alloreactivity and Predicts Inferior Graft Outcome
M. Meneghini¹, E. Crespo², M. Niemann³, A. Torija², E. Melilli⁴, M. Jarque², N. Montero⁴, J. Cruzado¹, J. Grinyo¹, O. Bestard¹
¹Kidney Transplantation Unit, Bellvitge University Hospital- IDIBELL, L'Hospitalet de Llobregat. Barcelona, Spain, ²Experimental Nephrology Laboratory, IDIBELL, L'Hospitalet de Llobregat. Barcelona, Spain, ³PIRCHE AG, Berlin, Germany, ⁴Kidney Transplantation Unit, Bellvitge University Hospital, L'Hospitalet de Llobregat. Barcelona, Spain
*Purpose: PIRCHE-II algorithm predicts mismatched donor-derived HLA peptides number presented on recipient HLAclass-II molecules to recipient T-cells (indirect pathway of allorecognition) and has been associated…
2019 American Transplant Congress
“Desens Light”: Single Center Experience Using Rituximab for High Immunologic Risk Kidney Transplant Recipients with Historic DSA
A. Casillas-Abundis, A. Tambur, A. Shetty, C. D'Agostino, J. R. Leventhal
Comprehensive Transplant Center, Northwestern Memorial Hospital, Chicago, IL
*Purpose: To evaluate the effectiveness of rituximab as induction therapy for pts undergoing kidney transplant (Tx) with historic DSA (hDSA) [Desens. Light].*Methods: 104 consecutive pts…