ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Computer-Assisted Cardiac Histologic Evaluation (CACHE) for Grading Cardiac Allograft Rejection

E. Peyster1, S. ArabYarmohommadi2, A. Janowczyk2, M. Sekulic3, C. Cassol4, M. Feldman1, K. Margulies1, A. Madabhushi2

1University of Pennsylvania, Philadelphia, PA, 2Case Western Reserve University, Cleveland, OH, 3University Hospitals Cleveland Medical Center, Cleveland, OH, 4Ohio State University, Columbus, OH

Meeting: 2020 American Transplant Congress

Abstract number: 77

Keywords: Heart, Histology, Image analysis, Rejection

Session Information

Date: Saturday, May 30, 2020

Session Name: Heart Transplantation: Antibodies and More

Session Time: 3:15pm-4:45pm

 Presentation Time: 3:39pm-3:51pm

Location: Virtual

Related Abstracts
  • Epigenetic Analysis of the TSDR of FOXP3 Demonstrates That Natural Treg Infiltrate the Cardiac Allograft Already before an Acute Rejection Episode
  • Patterns of Rejection in Murine, Porcine, and Human Vascularized Composite Allografts Based on Clinical, Histologic, and Immunohistochemical Analysis

*Purpose: Cardiac allograft rejection (CAR) is a serious concern in heart transplantation, occurring in 30-40% of patients during the first year. Endomyocardial biopsy (EMB) with qualitative histologic grading is the guideline-directed standard for CAR diagnosis, but the clinical and research utility of this standard is hindered by poor inter-pathologist agreement. A more reliable and reproducible approach to CAR grading would enhance the quality of inter-facility research and could provide valuable clinical decision support. Here, we use computer-vision algorithms to develop an automated pipeline for reproducible CAR grading, and then validate it via comparison to expert pathologists in a large, multicenter cohort.

*Methods: The study consisted of 1108 digitized EMB slides from three transplant centers. A training-set of 789 slides was used to develop the ‘CACHE-grader’ using a ‘hand-crafted’ feature extraction approach (Fig 1). Starting with segmentation of lymphocytes, myocytes, and interstitium, additional features to identify lymphocyte foci and to resolve tissue compartments for regional characterization were added. Overall, we extracted and modeled 127 quantitative morphologic features to optimize CAR grade assignment. The CACHE-grader performance was validated via percent agreement with the historical grade of record in a test-set of 319 new slides, and then compared to the percent agreement between study pathologists performing re-grading to test for non-inferiority.

*Results: The CACHE-grader demonstrated 73.9% (95% CI: 68.8-78.7%) agreement with the historical CAR grade of record. The overall pair-wise inter-grader agreement amongst study pathologist was 57.1% (95% CI: 50.9-63.3%). The CACHE-grader met the threshold for non-inferiority to human pathologists with regards to inter-grader-agreement.

*Conclusions: These results suggest that quantitative morphologic features extracted from EMB histology slides are predictive of rejection grades. Moreover, the CACHE-grader platform developed in this work met a threshold of non-inferiority, agreeing with expert pathologists as often as they agree with one another. This work demonstrates the potential of computational histologic analysis platforms to reproduce clinical-quality diagnostic performance, with potential for standardizing grading across centers and as clinical decision support.

 border=

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Peyster E, ArabYarmohommadi S, Janowczyk A, Sekulic M, Cassol C, Feldman M, Margulies K, Madabhushi A. Computer-Assisted Cardiac Histologic Evaluation (CACHE) for Grading Cardiac Allograft Rejection [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/computer-assisted-cardiac-histologic-evaluation-cache-for-grading-cardiac-allograft-rejection/. Accessed January 18, 2021.

« Back to 2020 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.