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A Single-Cell Transcriptome Atlas of 15 Adult Human Organs

Z. Guo1, S. He2, L. Wang1, Y. Liu2, J. Xu1, J. Bei2, X. He1

1Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China, 2The Cancer Centre of Sun Yat-Sen University, Guangzhou, China

Meeting: 2020 American Transplant Congress

Abstract number: D-345

Keywords: Gene expression, Lymphocytes, T cell receptors (TcR), Transcription factors

Session Information

Session Name: Poster Session D: Lymphocyte Biology: Signaling, Co-Stimulation, Regulation

Session Type: Poster Session

Date: Saturday, May 30, 2020

Session Time: 3:15pm-4:00pm

 Presentation Time: 3:30pm-4:00pm

Location: Virtual

*Purpose: The transcriptome diversity of cell types within and across tissues is of great importance for biological function of human beings. Here we conducted a systematic analysis of cell similarity and heterogeneity across multiple organs of healthy human adults

*Methods: We performed single-cell RNA, T cell receptor (TCR) and B cell receptor (BCR) sequencing on 15 organs from one adult male. The sequencing data was used to revealed annotation, similarities, differences and potential communications between different cell types

*Results: We isolated single cells using enzymatic digestion and selected all single live cells (Fig. 1A). 91393 cells were captured and sub-clustered into 43 subsets (Fig.1B & 1C). 20494 T cells were classified into CD4+ (11 clusters) and CD8+ T cells (22 clusters) according gene profiles (Fig. 2A & 2B). Trajectory and transcription factors analysis of CD4+ and CD8+ T cells revealed their special transcriptional regulation and differentiation mechanism. Meanwhile, TCR analysis of CD4+ T cell showed the different clone types and lower expansion ability than CD8+ (Fig. 2 C-I). We performed independent analyses on B cells, monocytes, macrophages, epithelial cells, endothelial cells, fibroblasts and smooth muscle cells. The molecular interactions and ligation-receptor pairs analysis revealed 468 different potential interactions in 15 tissues with 20694 combinations between cell types and established the broad intercellular communication networks within tissues (Fig. 3)

*Conclusions: Our dataset provides a resource for transcriptome characteristics of multiple organs at the single-cell level. This high-resolution adult human cellular atlas (AHCA) provides a foundation of knowledge to study biology of normal human cells and development of diseases

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To cite this abstract in AMA style:

Guo Z, He S, Wang L, Liu Y, Xu J, Bei J, He X. A Single-Cell Transcriptome Atlas of 15 Adult Human Organs [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/a-single-cell-transcriptome-atlas-of-15-adult-human-organs/. Accessed May 9, 2025.

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