Background. In kidney transplant recipients, IgG3 and IgG4 DSA have been associated with shorter time to acute AMR and with later allograft injury (glomerulopathy & IFTA), respectively. We were interested to determine the IgG subtypes circulating in a group of 46 adult (age and gender) potential kidney transplant recipients (PTR) with DSA to their potential living donors. All of them were CDC CxM –ive and FxM +ve or –ive. Methods. Class I and Class II HLA-DSA were determined by Luminex SAB (≥500 MFI = +ive). DSA IgG 1-4 subclasses were evaluated using monoclonal antibodies specific for IgG subclasses, as previously reported. All sera samples were tested the same day using cryopreserved (-70 0 C) sera from PTR containing DSAs against their respective donors. Results. Table 1 displays relevant findings of IgG subclasses and DSAs according to the FxM result.

Figure 1 shows the relation between MFI level (DSA) and IgG subclasses grouped (+ive or –ive). A total of 86 DSA were detected, only 23 of them (26.7 %) were IgG subclasses identified, which highly correlates with DSA MFI.

Conclusions. A lower number of IgG subclasses were identified compared to the frequency reported in AMR patients. It is tempting to speculate that the absence of continuous antigenic stimulus is responsible for a lower level of detection of the IgG subclasses.

CITATION INFORMATION: Contreras A, Casillas A, Llorente L, Lima G, Arvizu A, De Santiago A, Vilatoba M, Alberu J, Arreola-Guerra J. IgG Antibody Subclasses in Potential Renal Transplant Recipients with DSA, AHG-CDC CxM Negative and FxM Positive or Negative with Their Potential Donors. Am J Transplant. 2017;17 (suppl 3).

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