Articles tagged "Non-invasive diagnosis"

2022 American Transplant Congress
Urinary Cell mRNA Profile Diagnosis of Borderline TCMR in Kidney Allografts
T. Salinas¹, C. Li¹, C. Snopkowski¹, K. Chen¹, S. Albakry¹, S. P. Salvatore², S. Seshan², J. R. Lee¹, M. Thangamani¹, D. Dadhania¹, M. Suthanthiran¹
¹Nephrology, Weill Cornell-NYPH, New York, NY, ²Pathology, Weill Cornell-NYPH, New York, NY
*Purpose: Borderline T cell-mediated rejection (BR) is associated with inferior outcomes. In the multicenter CTOT-04 study, we discovered and validated a urinary cell three-gene signature…
2022 American Transplant Congress
Clinical Validation of Automated Urinary Chemokine Assays for Non-Invasive Detection of Kidney Transplant Rejection: A Large Prospective Cohort Study
E. Van Loon¹, C. Tinel¹, J. Callemeyn¹, M. Coemans¹, A. Van Craenenbroeck¹, T. Vaulet², D. Anglicheau³, M. Naesens¹
¹Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium, ²ESAT STADIUS Center for Dynamical Systems, KU Leuven, Leuven, Belgium, ³Department of Nephrology and Kidney Transplantation, Necker Hospital, Paris, France
*Purpose: Non-invasive diagnostic markers for kidney transplant rejection can provide useful information but often do not reach the clinical implementation stage. The chemokines CXCL9 and…
2022 American Transplant Congress
Development and Validation of a Machine Learning Based Virtual Biopsy System in Kidney Transplant Patients
D. Yoo¹, G. Divard¹, M. Raynaud¹, A. Bentall², M. Stegall³, M. Naesens⁴, I. Batal⁵, S. Coley⁶, J. Gill⁷, M. Mengel⁸, L. Cornell³, M. P. Alexander², T. Coates⁹, C. Legendre¹⁰, P. Reese¹¹, c. lefaucheur¹², O. Aubert¹, A. Loupy¹
¹Paris Transplant Group, Paris, France, ²-, -, AL, ³Mayo Clinic, Rochester, MN, ⁴Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium, ⁵Columbia University Medical Center, Scarsdale, NY, ⁶Columbia University, New York, NY, ⁷University of British Columbia, Vancouver, BC, Canada, ⁸University of Alberta, Edmonton, AB, Canada, ⁹University of Adelaide, Adelaide, Australia, ¹⁰Hopital Necker, Paris, France, ¹¹University of Pennsylvania, Philadelphia, PA, ¹²Hôpital Saint-Louis, Paris, France
*Purpose: In kidney TX, day-zero biopsies are used to assess organ quality and discriminate lesions inherited from the donor or acquired after transplantation. However, many…
2022 American Transplant Congress
Urinary Cell CTOT-04 Signature is Diagnostic of Glomerulitis in Kidney Allograft Biopsies
T. Salinas¹, C. Li¹, C. Snopkowski¹, K. Chen¹, S. Albakry¹, S. Salvatore², S. Seshan², J. R. Lee¹, M. Thangamani¹, D. Dadhania¹, M. Suthanthiran¹
¹Nephrology, Weill Cornell-NYPH, New York, NY, ²Pathology, Weill Cornell-NYPH, New York, NY
*Purpose: Antibody mediated rejection is a significant complication after kidney transplantation and is an important cause of allograft failure. In the multicenter CTOT-04 study, we…
2022 American Transplant Congress
Correlation of Donor-Derived Cell-Free DNA with Histology and Molecular Diagnoses of T-cell Mediated Rejection in Kidney Transplant Biopsies
D. Kumar¹, I. Moinuddin¹, L. Kamal¹, M. Shinbashi¹, N. Raju¹, R. Minniti¹, F. Moran¹, P. Halloran², G. Gupta¹
¹Virginia Commonwealth University, Richmond, VA, ²University of Alberta, Edmonton, AB, Canada
*Purpose: We have reported the correlation of donor-derived cell-free DNA (dd-cfDNA) with histologic and molecular diagnosis (MMDx) of kidney transplant biopsies (Gupta et al, Transplantation…
2022 American Transplant Congress
Diagnostic Performance of Donor-Derived Cell-Free DNA Assay (AlloSure®) in Kidney Transplant Recipients with Graft Dysfunction: A Single Center Study
M. S. Naseer¹, H. Shokouh-Amiri², T. Creamer¹, R. McMillan², S. Tandukar¹, D. Aultman¹, G. B. Zibari¹, N. Singh¹
¹Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA, ²John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA
*Purpose: Circulating donor-derived cell-free DNA (dd-cf-DNA) is a non-invasive biomarker of kidney allograft injury with a high negative predictive value for ruling out active rejection…
2021 American Transplant Congress
A Noninvasive Diagnostic Approach for Molecular Monitoring of Face Transplant Recipients
C. Snopkowski¹, P. Rabbani², H. Yang¹, Z. Berman², G. Diep², C. Li¹, T. Muthukumar¹, R. Ding¹, D. Ceradini², M. Suthanthiran¹
¹Weill Cornell Medical Institute, Department of Medicine, Division of Nephrology, NY, NY, ²NYU Langone Health, Hansjörg Wyss Department of Plastic Surgery, NY, NY
*Purpose: Skin biopsies are currently the gold standard for evaluating the inflammatory status of face allografts. However, “tape stripping” is emerging as a noninvasive technique…
2021 American Transplant Congress
Donor-Derived Cell-Free DNA as a Surrogate Marker for “Allograft Quiescence” After Kidney Transplantation
J. S. Bromberg¹, L. Bu², G. Gupta³, I. Moinuddin³, S. Anand⁴, A. Pai⁵, T. Alhamad⁶, V. Bowers⁷, S. Ghosh⁸, W. Tian⁸, E. Stites⁹
¹University of Maryland School of Medicine, Baltimore, MD, ²University of Minnesota, Minneapolis, MN, ³Virginia Commonwealth University, Richmond, VA, ⁴Intermountain Medical Center, Murray, UT, ⁵University of Texas McGovern Medical School, Houston, TX, ⁶Washington University in St. Louis, St. Louis, MO, ⁷Tampa General Hospital, Tampa, FL, ⁸CareDx, Brisbane, CA, ⁹University of Colorado, Aurora, CO
*Purpose: Donor-derived cell-free DNA (dd-cfDNA; AlloSure®) and the association with active rejection (AR) was described by Bloom et al, however subsequent insights have suggested this…
2021 American Transplant Congress
A Multimodal Interrogation of Human Renal Allografts
D. Dadhania¹, R. Ding¹, H. Xu², C. Lee¹, C. Snopkowski¹, T. Salinas¹, T. Muthukumar¹, J. Lee¹, R. Woodward², M. Grskovic², S. Dholakia², M. Suthanthiran¹
¹Weill Cornell Medicine - NYPH, New York, NY, ²CareDx, New York, NY
*Purpose: Development and validation of noninvasive biomarkers of kidney allograft rejection should improve transplant outcomes. CTOT-04 study validated urinary cell mRNA signature (CTOT04-Sig) for T-cell…
2021 American Transplant Congress
Association of Donor-Derived Cell-Free DNA with Severity of Interstitial Fibrosis and Cortical Atrophy Lesions Following Kidney Transplantation
L. Bu¹, E. Stites², V. Bowers³, T. Alhamad⁴, J. S. Bromberg⁵, G. Gupta⁶, I. Moinuddin⁶, S. Ghosh⁷, W. Tian⁷, A. Pai⁸, S. Anand⁹
¹University of Minnesota, Minneapolis, MN, ²University of Colorado, Aurora, CO, ³Tampa General Hospital, Tampa, FL, ⁴Washington University in St. Louis, St. Louis, MO, ⁵University of Maryland School of Medicine, Baltimore, MD, ⁶Virginia Commonwealth University, Richmond, VA, ⁷CareDx, Brisbane, CA, ⁸University of Texas McGovern Medical School, Houston, TX, ⁹Intermountain Medical Center, Murray, UT
*Purpose: The most common cause of kidney transplant (KT) failure is the poorly characterized histopathologic entity “interstitial fibrosis and tubular atrophy” (IFTA). There are no…