Articles tagged "Non-invasive diagnosis"

2022 American Transplant Congress
A Sign of Things to Come? Clinical Markers After Elevated Donor-Derived Cell-Free DNA in Heart Transplant Recipients
D. T. Kim¹, E. J. Henricksen², B. M. Zhang³, J. J. Teuteberg¹, Y. Moayedi⁴, H. I. Luikart¹, J. Han⁵, B. Wayda¹, K. Khush¹
¹Cardiology, Stanford University, Stanford, CA, ²Transplant, Stanford Health Care, Stanford, CA, ³Pathology, Stanford University, Stanford, CA, ⁴Cardiology, University Health Network, Toronto, ON, Canada, ⁵Cardiology, University of Chicago, Chicago, IL
*Purpose: Endomyocardial biopsy (EMBx) has historically been the standard of care for surveillance post heart transplant (HTx), but is limited by sample area and interpretation.…
2022 American Transplant Congress
Nonadherence Post-Kidney Transplant is Associated with Increased Immune Activation Detected by Peripheral Blood Gene Expression Assay
M. S. Naseer, H. Shokouh-Amiri, T. Creamer, R. McMillan, S. Tandukar, D. Aultman, G. B. Zibari, N. Singh
Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA
*Purpose: Nonadherence to immunosuppressive medications is common post-transplantation. We hypothesized that nonadherence results in a heightened alloimmune response in kidney transplant recipients (KTRs), which may…
2022 American Transplant Congress
dd-cfDNA Varies Among Diagnostics Groups of Pulmonary AMR
D. Levine¹, C. Giner¹, C. Pham², R. Woodward³, A. Roux⁴
¹University of Texas Health System San Antonio, San Antonio, TX, ²CareDX, South San Francisco, CA, ³CareDx, South San Francisco, CA, ⁴Pneumology, Adult CF Center and Lung Transplantation Department, Foch Hospital, Suresnes, France
*Purpose: Antibody mediated rejection (AMR) in lung transplant remains complex, with differential diagnosis often overlapping with other causes of lung dysfunction. Diagnostic certainty declines to…
2022 American Transplant Congress
The Unique Course of dd-cfDNA After Lung Transplant
D. Levine¹, C. Giner¹, C. Pham², R. Woodward³, A. Roux⁴
¹University of Texas Health System San Antonio, San Antonio, TX, ²CareDX, South San Francisco, CA, ³CareDx, South San Francisco, CA, ⁴Pneumology, Adult CF Center and Lung Transplantation Department, Foch Hospital, Suresnes, France
*Purpose: The time necessary for dd-cfDNA to reach baseline levels following transplantation has been reported in kidney, heart, and liver as 14-21 days. We explored…
2022 American Transplant Congress
Urinary Cell mRNA Profile Diagnosis of Borderline TCMR in Kidney Allografts
T. Salinas¹, C. Li¹, C. Snopkowski¹, K. Chen¹, S. Albakry¹, S. P. Salvatore², S. Seshan², J. R. Lee¹, M. Thangamani¹, D. Dadhania¹, M. Suthanthiran¹
¹Nephrology, Weill Cornell-NYPH, New York, NY, ²Pathology, Weill Cornell-NYPH, New York, NY
*Purpose: Borderline T cell-mediated rejection (BR) is associated with inferior outcomes. In the multicenter CTOT-04 study, we discovered and validated a urinary cell three-gene signature…
2022 American Transplant Congress
Clinical Validation of Automated Urinary Chemokine Assays for Non-Invasive Detection of Kidney Transplant Rejection: A Large Prospective Cohort Study
E. Van Loon¹, C. Tinel¹, J. Callemeyn¹, M. Coemans¹, A. Van Craenenbroeck¹, T. Vaulet², D. Anglicheau³, M. Naesens¹
¹Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium, ²ESAT STADIUS Center for Dynamical Systems, KU Leuven, Leuven, Belgium, ³Department of Nephrology and Kidney Transplantation, Necker Hospital, Paris, France
*Purpose: Non-invasive diagnostic markers for kidney transplant rejection can provide useful information but often do not reach the clinical implementation stage. The chemokines CXCL9 and…
2021 American Transplant Congress
Comparison of Next Generation Sequencing Donor-derived Cell Free DNA Assay Utilizing Whole Genome Sequencing
N. Singh¹, M. S. Naseer¹, D. Aultman², H. Shokouh-Amiri², G. Zibari²
¹Transplant, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA, ²Advanced Surgery, John C. McDonald Regional Transplant Center - Willis Knighton Health System, Shreveport, LA
*Purpose: Circulating donor-derived cell free DNA (dd-cfDNA) is a non-invasive biomarker of kidney allograft injury with a high negative predictive value for ruling out acute…
2021 American Transplant Congress
Use of Microbial Cell Free DNA Sequencing in Solid Organ Transplant Patients
S. Chang, P. Gaynor, A. Multani, O. E. Beaird, M. Carlson, S. Yang, O. Garner, J. Schaenman
University of California, Los Angeles, Los Angeles, CA
*Purpose: Microbial cell free DNA (cfDNA) sequencing has emerged as a new noninvasive tool for the diagnosis of infectious diseases in recent years, but its…
2021 American Transplant Congress
Clinical Validation and Performance of Donor-Derived Cell-Free DNA in Allograft Rejection
S. Anand¹, A. Pai², J. S. Bromberg³, G. Gupta⁴, I. Moinuddin⁴, T. Alhamad⁵, V. Bowers⁶, S. Ghosh⁷, W. Tian⁷, L. Bu⁸, E. Stites⁹
¹Intermountain Medical Center, Murray, UT, ²University of Texas McGovern Medical School, Houston, TX, ³University of Maryland School of Medicine, Baltimore, MD, ⁴Virginia Commonwealth University, Richmond, VA, ⁵Washington University in St. Louis, St. Louis, MO, ⁶Tampa General Hospital, Tampa, FL, ⁷CareDx, Brisbane, CA, ⁸University of Minnesota, Minneapolis, MN, ⁹University of Colorado, Aurora, CO
*Purpose: Extensive literature regarding the utility of donor-derived cell-free DNA (dd-cfDNA) shares various thresholds for active rejection (AR) based on differing data sets for both…
2021 American Transplant Congress
Plasma Donor-Derived Cell-Free DNA Levels Risk-Stratify Kidney Allograft Injury with Isolated Transplant Glomerulitis
L. Bu¹, S. Anand², A. Pai³, J. S. Bromberg⁴, G. Gupta⁵, I. Moinuddin⁵, T. Alhamad⁶, V. Bowers⁷, S. Ghosh⁸, W. Tian⁸, E. Stites⁹
¹University of Minnesota, Minneapolis, MN, ²Intermountain Medical Center, Murray, UT, ³University of Texas McGovern Medical School, Houston, TX, ⁴University of Maryland School of Medicine, Baltimore, MD, ⁵Virginia Commonwealth University, Richmond, VA, ⁶Washington University in St. Louis, St. Louis, MO, ⁷Tampa General Hospital, Tampa, FL, ⁸CareDx, Brisbane, CA, ⁹University of Colorado, Aurora, CO
*Purpose: The Banff lesion Glomerulitis (g) characterizes the degree of microvascular glomerular inflammation. Some studies have demonstrated glomerulitis in the absence of C4d in association…