2018 American Transplant Congress
Immunity and Tolerance after Bilateral Orthotopic Lung Transplant (BOLT) in Tandem with a CD3+/CD19+ Depleted Vertebral Bone Marrow Transplant (BOLT+BMT) from 1 of 8 HLA-Matched Cadaveric Donors
Primary immunodeficiency patients may develop pulmonary complications and most are ineligible for either lung transplant or BMT due to futility. We report our first 2…2018 American Transplant Congress
Absolute Quantification of Plasma Graft-Derived Cell-Free DNA for Diagnosis of Kidney Transplant Rejection
Aim: To study the performance of droplet digital PCR absolute measurement of graft-derived cell-free DNA (gdcfDNA) for the diagnosis of kidney allograft rejection.Methods: Homozygous deletion…2018 American Transplant Congress
A Novel Conditioning Approach to Establish Chimerism Using Manipulation of JAK-STAT Pathways
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY.
Hematopoietic chimerism establishes donor-specific tolerance to solid organ allografts. Targeting recipient immune cells (T, NK cells and macrophages) can effectively reduce the dose of total…2018 American Transplant Congress
Tomotherapy and Donor Hematopoietic Cells for Tolerance to Kidney Transplants in Rhesus Macaques
The goal of this project was to develop a tolerance induction protocol for MHC 1-haplotype mismatched kidney transplants (txs) in a rhesus macaque model. The…2017 American Transplant Congress
Induction of Mixed Chimerism to Prolong Liver Allograft Survival in Cynomolgus Monkeys.
CCTI, Columbia University, New York, NY
Purpose: Spontaneous liver tolerance occurs in highly selected patients with stable liver function. There is an unmet need for a safe and reliable regimen allowing…2017 American Transplant Congress
Dual Costimulatory Blockades Failed to Achieve Transplant Tolerance Despite Induction of Mixed Chimerism in NHPs.
Background: Our previous studies showed that substantial deletion of CD8+ memory T cells (TMEM) was necessary to induce mixed chimerism (MC) and allograft tolerance when…2017 American Transplant Congress
Combining Neural Stem Cell Transplantation with Mixed Hematopoietic Chimerism Promotes Restoration of Self-Tolerance in Established Experimental Autoimmune Encephalomyelitis.
Surgery, Massachusetts General Hospital, Boston, MA
Multilineage mixed hematopoietic chimerism induces tolerance to alloantigens in rodents, large animals and kidney-transplanted patients. Here we combined mixed chimerism with neural stem cell (NSC)…2017 American Transplant Congress
Ex vivo Expanded Regulatory T cells Combined with Short-Term Costimulation Blockade Prevent Rejection of Vascularized Composite Allografts.
Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, Baltimore, MD
[Background] Reconstructive transplantation represents a valid therapeutic option after devastating tissue loss. Routine clinical application, however, is hampered by the toxicity of long-term maintenance immunosuppression.…2017 American Transplant Congress
CTLA4-Ig, Vascularized Bone Marrow and Donor Bone Marrow Cells Successfully Negate the Development of Acute Skin Rejection of Vascularized Composite Allografts in MHC Class I Mismatched Recipients.
Massachusetts General Hospital, Boston
PURPOSE: Previously we have shown that MHC class I-mismatched recipient miniature swine (n=3) experienced rejection (from POD42 onwards) of the epidermal component of fasciocutaneous vascularized…2017 American Transplant Congress
The Intragraft Vascularized Bone Marrow Component and Recipient T Regulatory Cells Facilitate Tolerance Induction After Post Transplant High Dose Cyclophosphamide Treatment in VCA.
Johns Hopkins University School of Medicine, Baltimore, MD
Background: VCA is a viable treatment option for devastating tissue defects. However, the life-long need for immunosuppression curtails the wider use of VCA. Unique VCA-features…